Minoxidil delivered via a stem cell membrane delivery controlled release system promotes hair growth in C57BL/6J mice
Objective: Umbilical cord-derived mesenchymal stem cell membrane-loaded minoxidil (MXD) nanoparticles (STCM-MXD-NPs) were prepared to investigate their effects on hair growth in C57BL/6J mice.Methods: STCM-MXD-NPs were obtained by freeze-thawing and differential centrifugation, and their effects on...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1331754/full |
| _version_ | 1797362356800454656 |
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| author | Dandan Song Shouxi Pan Wenxia Jin Ronghui Wu Tianqi Zhao Jinlan Jiang Mingji Zhu |
| author_facet | Dandan Song Shouxi Pan Wenxia Jin Ronghui Wu Tianqi Zhao Jinlan Jiang Mingji Zhu |
| author_sort | Dandan Song |
| collection | DOAJ |
| description | Objective: Umbilical cord-derived mesenchymal stem cell membrane-loaded minoxidil (MXD) nanoparticles (STCM-MXD-NPs) were prepared to investigate their effects on hair growth in C57BL/6J mice.Methods: STCM-MXD-NPs were obtained by freeze-thawing and differential centrifugation, and their effects on hair growth were evaluated using C57BL/6J mice. The mRNA and protein expression levels of vascular endothelial growth factor (VEGF) and insulin-like growth factor-1 (IGF-1) were detected by real-time polymerase chain reaction and enzyme-linked immunosorbent assays, respectively. Protein expression levels of marker of proliferation Ki-67 (MKI67) and β-catenin (CTNNB) in skin tissue were detected by immunohistochemistry.Results: STCM-MXD-NPs improved MXD solubility. They released the drug slowly, increasing its transdermal properties, accumulation in the skin, and content in the hair bulb tissues with a better efficacy than that of ordinary MXD. Moreover, STCM-MXD-NPs significantly upregulated the mRNA and protein levels of VEGF and IGF-1 and promoted the protein expression of MKI67 and CTNNB in mouse skin tissues, promoting mouse hair growth.Conclusion: Stem cell membrane-loaded MXD nanoparticles with slow-release properties increased MXD accumulation in the skin by improving its transdermal properties, increasing VEGF, IGF-1, MKI67, and CTNNB expression levels and promoting hair growth in C57BL/6J mice. |
| first_indexed | 2024-03-08T16:06:51Z |
| format | Article |
| id | doaj.art-ddd12c8e4eea4f0caf5a2c4cef6cfd27 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-08T16:06:51Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-ddd12c8e4eea4f0caf5a2c4cef6cfd272024-01-08T05:54:12ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-01-011110.3389/fbioe.2023.13317541331754Minoxidil delivered via a stem cell membrane delivery controlled release system promotes hair growth in C57BL/6J miceDandan Song0Shouxi Pan1Wenxia Jin2Ronghui Wu3Tianqi Zhao4Jinlan Jiang5Mingji Zhu6Department of Dermatology, China-Japan Union Hospital of Jilin University, Changchun, ChinaNorman Bethune College of Medicine, Jilin University, Changchun, ChinaLanzhou Institute of Biological Products Co., Ltd., Lanzhou, ChinaDepartment of Dermatology, China-Japan Union Hospital of Jilin University, Changchun, ChinaDepartment of Dermatology, China-Japan Union Hospital of Jilin University, Changchun, ChinaScientific Research Center, China-Japan Union Hospital of Jilin University, Changchun, ChinaDepartment of Dermatology, China-Japan Union Hospital of Jilin University, Changchun, ChinaObjective: Umbilical cord-derived mesenchymal stem cell membrane-loaded minoxidil (MXD) nanoparticles (STCM-MXD-NPs) were prepared to investigate their effects on hair growth in C57BL/6J mice.Methods: STCM-MXD-NPs were obtained by freeze-thawing and differential centrifugation, and their effects on hair growth were evaluated using C57BL/6J mice. The mRNA and protein expression levels of vascular endothelial growth factor (VEGF) and insulin-like growth factor-1 (IGF-1) were detected by real-time polymerase chain reaction and enzyme-linked immunosorbent assays, respectively. Protein expression levels of marker of proliferation Ki-67 (MKI67) and β-catenin (CTNNB) in skin tissue were detected by immunohistochemistry.Results: STCM-MXD-NPs improved MXD solubility. They released the drug slowly, increasing its transdermal properties, accumulation in the skin, and content in the hair bulb tissues with a better efficacy than that of ordinary MXD. Moreover, STCM-MXD-NPs significantly upregulated the mRNA and protein levels of VEGF and IGF-1 and promoted the protein expression of MKI67 and CTNNB in mouse skin tissues, promoting mouse hair growth.Conclusion: Stem cell membrane-loaded MXD nanoparticles with slow-release properties increased MXD accumulation in the skin by improving its transdermal properties, increasing VEGF, IGF-1, MKI67, and CTNNB expression levels and promoting hair growth in C57BL/6J mice.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1331754/fullminoxidilstem cell membranenanoparticlehair follicle deliveryhair growth |
| spellingShingle | Dandan Song Shouxi Pan Wenxia Jin Ronghui Wu Tianqi Zhao Jinlan Jiang Mingji Zhu Minoxidil delivered via a stem cell membrane delivery controlled release system promotes hair growth in C57BL/6J mice Frontiers in Bioengineering and Biotechnology minoxidil stem cell membrane nanoparticle hair follicle delivery hair growth |
| title | Minoxidil delivered via a stem cell membrane delivery controlled release system promotes hair growth in C57BL/6J mice |
| title_full | Minoxidil delivered via a stem cell membrane delivery controlled release system promotes hair growth in C57BL/6J mice |
| title_fullStr | Minoxidil delivered via a stem cell membrane delivery controlled release system promotes hair growth in C57BL/6J mice |
| title_full_unstemmed | Minoxidil delivered via a stem cell membrane delivery controlled release system promotes hair growth in C57BL/6J mice |
| title_short | Minoxidil delivered via a stem cell membrane delivery controlled release system promotes hair growth in C57BL/6J mice |
| title_sort | minoxidil delivered via a stem cell membrane delivery controlled release system promotes hair growth in c57bl 6j mice |
| topic | minoxidil stem cell membrane nanoparticle hair follicle delivery hair growth |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1331754/full |
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