Novel Salinity Tolerance Loci in Chickpea Identified in Glasshouse and Field Environments
A better understanding of the genetics of salinity tolerance in chickpea would enable breeding of salt tolerant varieties, offering potential to expand chickpea production to marginal, salinity-affected areas. A Recombinant Inbred Line population was developed using accelerated-Single Seed Descent o...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-04-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.667910/full |
| _version_ | 1818601392210706432 |
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| author | Judith Atieno Judith Atieno Timothy D. Colmer Julian Taylor Yongle Li John Quealy Lukasz Kotula Dion Nicol Dion Nicol Duong T. Nguyen Duong T. Nguyen Chris Brien Peter Langridge Janine Croser Julie E. Hayes Tim Sutton Tim Sutton |
| author_facet | Judith Atieno Judith Atieno Timothy D. Colmer Julian Taylor Yongle Li John Quealy Lukasz Kotula Dion Nicol Dion Nicol Duong T. Nguyen Duong T. Nguyen Chris Brien Peter Langridge Janine Croser Julie E. Hayes Tim Sutton Tim Sutton |
| author_sort | Judith Atieno |
| collection | DOAJ |
| description | A better understanding of the genetics of salinity tolerance in chickpea would enable breeding of salt tolerant varieties, offering potential to expand chickpea production to marginal, salinity-affected areas. A Recombinant Inbred Line population was developed using accelerated-Single Seed Descent of progeny from a cross between two chickpea varieties, Rupali (salt-sensitive) and Genesis836 (salt-tolerant). The population was screened for salinity tolerance using high-throughput image-based phenotyping in the glasshouse, in hydroponics, and across 2 years of field trials at Merredin, Western Australia. A genetic map was constructed from 628 unique in-silico DArT and SNP markers, spanning 963.5 cM. Markers linked to two flowering loci identified on linkage groups CaLG03 and CaLG05 were used as cofactors during genetic analysis to remove the confounding effects of flowering on salinity response. Forty-two QTL were linked to growth rate, yield, and yield component traits under both control and saline conditions, and leaf tissue ion accumulation under salt stress. Residuals from regressions fitting best linear unbiased predictions from saline conditions onto best linear unbiased predictions from control conditions provided a measure of salinity tolerance per se, independent of yield potential. Six QTL on CaLG04, CaLG05, and CaLG06 were associated with tolerance per se. In total, 21 QTL mapped to two distinct regions on CaLG04. The first distinct region controlled the number of filled pods, leaf necrosis, seed number, and seed yield specifically under salinity, and co-located with four QTL linked to salt tolerance per se. The second distinct region controlled 100-seed weight and growth-related traits, independent of salinity treatment. Positional cloning of the salinity tolerance-specific loci on CaLG04, CaLG05, and CaLG06 will improve our understanding of the key determinants of salinity tolerance in chickpea. |
| first_indexed | 2024-12-16T12:50:39Z |
| format | Article |
| id | doaj.art-ddd7f0088b3e404aa1502ba13cc00297 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-16T12:50:39Z |
| publishDate | 2021-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-ddd7f0088b3e404aa1502ba13cc002972022-12-21T22:31:10ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-04-011210.3389/fpls.2021.667910667910Novel Salinity Tolerance Loci in Chickpea Identified in Glasshouse and Field EnvironmentsJudith Atieno0Judith Atieno1Timothy D. Colmer2Julian Taylor3Yongle Li4John Quealy5Lukasz Kotula6Dion Nicol7Dion Nicol8Duong T. Nguyen9Duong T. Nguyen10Chris Brien11Peter Langridge12Janine Croser13Julie E. Hayes14Tim Sutton15Tim Sutton16South Australian Research and Development Institute, Adelaide, SA, AustraliaSchool of Agriculture, Food and Wine, University of Adelaide, Adelaide, SA, AustraliaSchool of Agriculture and Environment, The University of Western Australia, Perth, WA, AustraliaSchool of Agriculture, Food and Wine, University of Adelaide, Adelaide, SA, AustraliaSchool of Agriculture, Food and Wine, University of Adelaide, Adelaide, SA, AustraliaSchool of Agriculture and Environment, The University of Western Australia, Perth, WA, AustraliaSchool of Agriculture and Environment, The University of Western Australia, Perth, WA, AustraliaSchool of Agriculture and Environment, The University of Western Australia, Perth, WA, AustraliaDepartment of Primary Industries and Regional Development, Dryland Research Institute, South Perth, WA, AustraliaSouth Australian Research and Development Institute, Adelaide, SA, AustraliaSchool of Agriculture and Environment, The University of Western Australia, Perth, WA, AustraliaThe Plant Accelerator, Australian Plant Phenomics Facility, University of Adelaide, Adelaide, SA, AustraliaSchool of Agriculture, Food and Wine, University of Adelaide, Adelaide, SA, AustraliaSchool of Agriculture and Environment, The University of Western Australia, Perth, WA, AustraliaSchool of Agriculture, Food and Wine, University of Adelaide, Adelaide, SA, AustraliaSouth Australian Research and Development Institute, Adelaide, SA, AustraliaSchool of Agriculture, Food and Wine, University of Adelaide, Adelaide, SA, AustraliaA better understanding of the genetics of salinity tolerance in chickpea would enable breeding of salt tolerant varieties, offering potential to expand chickpea production to marginal, salinity-affected areas. A Recombinant Inbred Line population was developed using accelerated-Single Seed Descent of progeny from a cross between two chickpea varieties, Rupali (salt-sensitive) and Genesis836 (salt-tolerant). The population was screened for salinity tolerance using high-throughput image-based phenotyping in the glasshouse, in hydroponics, and across 2 years of field trials at Merredin, Western Australia. A genetic map was constructed from 628 unique in-silico DArT and SNP markers, spanning 963.5 cM. Markers linked to two flowering loci identified on linkage groups CaLG03 and CaLG05 were used as cofactors during genetic analysis to remove the confounding effects of flowering on salinity response. Forty-two QTL were linked to growth rate, yield, and yield component traits under both control and saline conditions, and leaf tissue ion accumulation under salt stress. Residuals from regressions fitting best linear unbiased predictions from saline conditions onto best linear unbiased predictions from control conditions provided a measure of salinity tolerance per se, independent of yield potential. Six QTL on CaLG04, CaLG05, and CaLG06 were associated with tolerance per se. In total, 21 QTL mapped to two distinct regions on CaLG04. The first distinct region controlled the number of filled pods, leaf necrosis, seed number, and seed yield specifically under salinity, and co-located with four QTL linked to salt tolerance per se. The second distinct region controlled 100-seed weight and growth-related traits, independent of salinity treatment. Positional cloning of the salinity tolerance-specific loci on CaLG04, CaLG05, and CaLG06 will improve our understanding of the key determinants of salinity tolerance in chickpea.https://www.frontiersin.org/articles/10.3389/fpls.2021.667910/fullchickpeasalt stresstissue Na+multiple environment phenotypinglinkage mappingQTL |
| spellingShingle | Judith Atieno Judith Atieno Timothy D. Colmer Julian Taylor Yongle Li John Quealy Lukasz Kotula Dion Nicol Dion Nicol Duong T. Nguyen Duong T. Nguyen Chris Brien Peter Langridge Janine Croser Julie E. Hayes Tim Sutton Tim Sutton Novel Salinity Tolerance Loci in Chickpea Identified in Glasshouse and Field Environments Frontiers in Plant Science chickpea salt stress tissue Na+ multiple environment phenotyping linkage mapping QTL |
| title | Novel Salinity Tolerance Loci in Chickpea Identified in Glasshouse and Field Environments |
| title_full | Novel Salinity Tolerance Loci in Chickpea Identified in Glasshouse and Field Environments |
| title_fullStr | Novel Salinity Tolerance Loci in Chickpea Identified in Glasshouse and Field Environments |
| title_full_unstemmed | Novel Salinity Tolerance Loci in Chickpea Identified in Glasshouse and Field Environments |
| title_short | Novel Salinity Tolerance Loci in Chickpea Identified in Glasshouse and Field Environments |
| title_sort | novel salinity tolerance loci in chickpea identified in glasshouse and field environments |
| topic | chickpea salt stress tissue Na+ multiple environment phenotyping linkage mapping QTL |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.667910/full |
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