RNA Sequencing Reveals the Potential Adaptation Mechanism to Different Hosts of <i>Grapholita molesta</i>
<i>Grapholita molesta</i> is an important fruit tree worldwide pest which feeds on hosts extensively and does serious harm. In this paper, the growth and development parameters and protease activities of <i>G. molesta</i> fed on different hosts were compared. Using Illumina R...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/10/893 |
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| author | Dongbiao Lü Zizheng Yan Di Hu Aiping Zhao Shujun Wei Ping Wang Xiangqun Yuan Yiping Li |
| author_facet | Dongbiao Lü Zizheng Yan Di Hu Aiping Zhao Shujun Wei Ping Wang Xiangqun Yuan Yiping Li |
| author_sort | Dongbiao Lü |
| collection | DOAJ |
| description | <i>Grapholita molesta</i> is an important fruit tree worldwide pest which feeds on hosts extensively and does serious harm. In this paper, the growth and development parameters and protease activities of <i>G. molesta</i> fed on different hosts were compared. Using Illumina RNA sequencing technology, 18 midgut samples from five different hosts (apple, pear, plum, peach and peach shoots) and artificial diet were sequenced and compared with the reference genome, resulting in 15269 genes and 2785 predicted new genes. From 15 comparative combinations, DEGs were found from 286 to 4187 in each group, with up-regulated genes from 107 to 2395 and down-regulated genes from 83 to 2665. KEGG pathway analysis showed that DEGs were associated with amino acid metabolism, starch and sucrose metabolism, carbohydrate metabolism, and hydrolase activity. A total of 31 co-expression gene modules of different hosts were identified by WGCNA. qRT-PCR showed that the expression pattern of the trypsin gene was consistent with RNA sequencing. In this study, growth and development parameters, protease activity, DEGs, enrichment analysis and qRT-PCR were combined to reveal the adaptation process to different hosts of <i>G. molesta</i> in many aspects. The results of this study provide a basis for further exploration of the molecular mechanism of host adaptation of <i>G. molesta.</i> |
| first_indexed | 2024-03-09T20:04:16Z |
| format | Article |
| id | doaj.art-ddd9c39ab42449bd82c0cc2e098b43ee |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T20:04:16Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-ddd9c39ab42449bd82c0cc2e098b43ee2023-11-24T00:37:50ZengMDPI AGInsects2075-44502022-09-01131089310.3390/insects13100893RNA Sequencing Reveals the Potential Adaptation Mechanism to Different Hosts of <i>Grapholita molesta</i>Dongbiao Lü0Zizheng Yan1Di Hu2Aiping Zhao3Shujun Wei4Ping Wang5Xiangqun Yuan6Yiping Li7Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, 9 Shuguanghuayuan Middle Road, Haidian District, Beijing 100097, ChinaDepartment of Entomology, Cornell University, Geneva, NY 14456, USAKey Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, ChinaKey Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau, Ministry of Agriculture, College of Plant Protection, Northwest A&F University, Yangling, Xianyang 712100, China<i>Grapholita molesta</i> is an important fruit tree worldwide pest which feeds on hosts extensively and does serious harm. In this paper, the growth and development parameters and protease activities of <i>G. molesta</i> fed on different hosts were compared. Using Illumina RNA sequencing technology, 18 midgut samples from five different hosts (apple, pear, plum, peach and peach shoots) and artificial diet were sequenced and compared with the reference genome, resulting in 15269 genes and 2785 predicted new genes. From 15 comparative combinations, DEGs were found from 286 to 4187 in each group, with up-regulated genes from 107 to 2395 and down-regulated genes from 83 to 2665. KEGG pathway analysis showed that DEGs were associated with amino acid metabolism, starch and sucrose metabolism, carbohydrate metabolism, and hydrolase activity. A total of 31 co-expression gene modules of different hosts were identified by WGCNA. qRT-PCR showed that the expression pattern of the trypsin gene was consistent with RNA sequencing. In this study, growth and development parameters, protease activity, DEGs, enrichment analysis and qRT-PCR were combined to reveal the adaptation process to different hosts of <i>G. molesta</i> in many aspects. The results of this study provide a basis for further exploration of the molecular mechanism of host adaptation of <i>G. molesta.</i>https://www.mdpi.com/2075-4450/13/10/893<i>Grapholita molesta</i>host speciesRNA sequencingadaptationtrypsin |
| spellingShingle | Dongbiao Lü Zizheng Yan Di Hu Aiping Zhao Shujun Wei Ping Wang Xiangqun Yuan Yiping Li RNA Sequencing Reveals the Potential Adaptation Mechanism to Different Hosts of <i>Grapholita molesta</i> Insects <i>Grapholita molesta</i> host species RNA sequencing adaptation trypsin |
| title | RNA Sequencing Reveals the Potential Adaptation Mechanism to Different Hosts of <i>Grapholita molesta</i> |
| title_full | RNA Sequencing Reveals the Potential Adaptation Mechanism to Different Hosts of <i>Grapholita molesta</i> |
| title_fullStr | RNA Sequencing Reveals the Potential Adaptation Mechanism to Different Hosts of <i>Grapholita molesta</i> |
| title_full_unstemmed | RNA Sequencing Reveals the Potential Adaptation Mechanism to Different Hosts of <i>Grapholita molesta</i> |
| title_short | RNA Sequencing Reveals the Potential Adaptation Mechanism to Different Hosts of <i>Grapholita molesta</i> |
| title_sort | rna sequencing reveals the potential adaptation mechanism to different hosts of i grapholita molesta i |
| topic | <i>Grapholita molesta</i> host species RNA sequencing adaptation trypsin |
| url | https://www.mdpi.com/2075-4450/13/10/893 |
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