Bacteriophages as potential treatment for urinary tract infections
Background: Urinary tract infections (UTIs) are among the most prevalent microbial diseases and their financial burden on society is substantial. The continuing increase of antibiotic resistance worldwide is alarming so that well-tolerated, highly effective therapeutic alternatives are urgently need...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00465/full |
| _version_ | 1818946042399293440 |
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| author | Wilbert eSybesma Reinhard eZbinden Nino eChanishvili Mzia eKutateladze Archil eChkhotua Aleksandre eUjmajuridze Ulrich eMehnert Thomas eKessler |
| author_facet | Wilbert eSybesma Reinhard eZbinden Nino eChanishvili Mzia eKutateladze Archil eChkhotua Aleksandre eUjmajuridze Ulrich eMehnert Thomas eKessler |
| author_sort | Wilbert eSybesma |
| collection | DOAJ |
| description | Background: Urinary tract infections (UTIs) are among the most prevalent microbial diseases and their financial burden on society is substantial. The continuing increase of antibiotic resistance worldwide is alarming so that well-tolerated, highly effective therapeutic alternatives are urgently needed.Objective: To investigate the effect of bacteriophages on E. coli and K. pneumoniae strains isolated from the urine of patients suffering from UTIs.Material and methods: 41 E. coli and 9 K. pneumoniae strains, isolated from the urine of patients suffering from UTIs, were tested in-vitro for their susceptibility towards bacteriophages. The bacteriophages originated from either commercially available bacteriophage cocktails registered in Georgia or from the bacteriophage collection of the George Eliava Institute of Bacteriophage, Microbiology and Virology. In-vitro screening of bacterial strains was performed by use of the spot-test method. The experiments were implemented 3 times by different groups of scientists.Results: The lytic activity of the commercial bacteriophage cocktails on the 41 E. coli strains varied between 66% (Pyo phage) and 93% (Enko phage). After bacteriophage adaptation of the Pyo phage cocktail, its lytic activity was increased from 66% to 93% and only one E. coli strain remained resistant. One bacteriophage of the Eliava collection could lyse all 9 K. pneumoniae strains. Conclusions: Based on the high lytic activity and the potential of resistance optimization by direct adaption of bacteriophages as reported in this study, and in view of the continuing increase of antibiotic resistance worldwide, bacteriophage therapy is a promising treatment option for UTIs highly warranting randomized controlled trials. |
| first_indexed | 2024-12-20T08:08:43Z |
| format | Article |
| id | doaj.art-dddb8a0d3d7e4838af89e3b757754940 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T08:08:43Z |
| publishDate | 2016-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-dddb8a0d3d7e4838af89e3b7577549402022-12-21T19:47:21ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-04-01710.3389/fmicb.2016.00465183439Bacteriophages as potential treatment for urinary tract infectionsWilbert eSybesma0Reinhard eZbinden1Nino eChanishvili2Mzia eKutateladze3Archil eChkhotua4Aleksandre eUjmajuridze5Ulrich eMehnert6Thomas eKessler7Balgrist University Hospital, Zürich, SwitzerlandUniversity of ZürichThe Eliava Institute of Bacteriophage, Microbiology, and VirologyThe Eliava Institute of Bacteriophage, Microbiology, and VirologyTsulukidze National Center of UrologyTsulukidze National Center of UrologyBalgrist University Hospital, Zürich, SwitzerlandBalgrist University Hospital, Zürich, SwitzerlandBackground: Urinary tract infections (UTIs) are among the most prevalent microbial diseases and their financial burden on society is substantial. The continuing increase of antibiotic resistance worldwide is alarming so that well-tolerated, highly effective therapeutic alternatives are urgently needed.Objective: To investigate the effect of bacteriophages on E. coli and K. pneumoniae strains isolated from the urine of patients suffering from UTIs.Material and methods: 41 E. coli and 9 K. pneumoniae strains, isolated from the urine of patients suffering from UTIs, were tested in-vitro for their susceptibility towards bacteriophages. The bacteriophages originated from either commercially available bacteriophage cocktails registered in Georgia or from the bacteriophage collection of the George Eliava Institute of Bacteriophage, Microbiology and Virology. In-vitro screening of bacterial strains was performed by use of the spot-test method. The experiments were implemented 3 times by different groups of scientists.Results: The lytic activity of the commercial bacteriophage cocktails on the 41 E. coli strains varied between 66% (Pyo phage) and 93% (Enko phage). After bacteriophage adaptation of the Pyo phage cocktail, its lytic activity was increased from 66% to 93% and only one E. coli strain remained resistant. One bacteriophage of the Eliava collection could lyse all 9 K. pneumoniae strains. Conclusions: Based on the high lytic activity and the potential of resistance optimization by direct adaption of bacteriophages as reported in this study, and in view of the continuing increase of antibiotic resistance worldwide, bacteriophage therapy is a promising treatment option for UTIs highly warranting randomized controlled trials.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00465/fullBacteriophagesantibioticsUrinary tract infectionBacteriophage adaptationantibiotic resistrance |
| spellingShingle | Wilbert eSybesma Reinhard eZbinden Nino eChanishvili Mzia eKutateladze Archil eChkhotua Aleksandre eUjmajuridze Ulrich eMehnert Thomas eKessler Bacteriophages as potential treatment for urinary tract infections Frontiers in Microbiology Bacteriophages antibiotics Urinary tract infection Bacteriophage adaptation antibiotic resistrance |
| title | Bacteriophages as potential treatment for urinary tract infections |
| title_full | Bacteriophages as potential treatment for urinary tract infections |
| title_fullStr | Bacteriophages as potential treatment for urinary tract infections |
| title_full_unstemmed | Bacteriophages as potential treatment for urinary tract infections |
| title_short | Bacteriophages as potential treatment for urinary tract infections |
| title_sort | bacteriophages as potential treatment for urinary tract infections |
| topic | Bacteriophages antibiotics Urinary tract infection Bacteriophage adaptation antibiotic resistrance |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00465/full |
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