In-Vivo LC-OCT Evaluation of the Downward Proliferation Pattern of Keratinocytes in Actinic Keratosis in Comparison with Histology: First Impressions from a Pilot Study
It is known that actinic keratoses (AKs) can progress to invasive squamous cell carcinoma (SCC). The histological PRO grading of AKs is based on the growth pattern of basal keratinocytes and relates to their progression risk. AKs can be non-invasively characterized by line-field confocal optical coh...
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MDPI AG
2021-06-01
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| Series: | Cancers |
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| author | Cristel Ruini Sandra Schuh Charlotte Gust Daniela Hartmann Lars Einar French Elke Christina Sattler Julia Welzel |
| author_facet | Cristel Ruini Sandra Schuh Charlotte Gust Daniela Hartmann Lars Einar French Elke Christina Sattler Julia Welzel |
| author_sort | Cristel Ruini |
| collection | DOAJ |
| description | It is known that actinic keratoses (AKs) can progress to invasive squamous cell carcinoma (SCC). The histological PRO grading of AKs is based on the growth pattern of basal keratinocytes and relates to their progression risk. AKs can be non-invasively characterized by line-field confocal optical coherence tomography (LC-OCT). The aim of the study was to define criteria for an LC-OCT grading of AKs based on the PRO classification and to correlate it with its histological counterpart. To evaluate the interobserver agreement for the LC-OCT PRO classification, fifty AKs were imaged by LC-OCT and biopsied for histopathology. PRO histological grading was assessed by an expert consensus, while two evaluator groups separately performed LC-OCT grading on vertical sections. The agreement between LC-OCT and histological PRO grading was 75% for all lesions (weighted kappa 0.66, 95% CI 0.48–0.83, <i>p</i> ≤ 0.001) and 85.4% when comparing the subgroups PRO I vs. PRO II/III (weighted kappa 0.64, 95% CI 0.40–0.88, <i>p</i> ≤ 0.001). The interobserver agreement for LC-OCT was 90% (Cohen’s kappa 0.84, 95% CI 0.71–0.91, <i>p</i> ≤ 0.001). In this pilot study, we demonstrated that LC-OCT is potentially able to classify AKs based on the basal growth pattern of keratinocytes, in-vivo reproducing the PRO classification, with strong interobserver agreement and a good correlation with histopathology. |
| first_indexed | 2024-03-10T10:37:33Z |
| format | Article |
| id | doaj.art-dddcc53371584a248732ee27eec67274 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T10:37:33Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-dddcc53371584a248732ee27eec672742023-11-21T23:12:16ZengMDPI AGCancers2072-66942021-06-011312285610.3390/cancers13122856In-Vivo LC-OCT Evaluation of the Downward Proliferation Pattern of Keratinocytes in Actinic Keratosis in Comparison with Histology: First Impressions from a Pilot StudyCristel Ruini0Sandra Schuh1Charlotte Gust2Daniela Hartmann3Lars Einar French4Elke Christina Sattler5Julia Welzel6Department of Dermatology and Allergy, University Hospital, LMU Munich, 80337 Munich, GermanyDepartment of Dermatology and Allergy, University Hospital, 86156 Augsburg, GermanyDepartment of Dermatology and Allergy, University Hospital, LMU Munich, 80337 Munich, GermanyDepartment of Dermatology and Allergy, University Hospital, LMU Munich, 80337 Munich, GermanyDepartment of Dermatology and Allergy, University Hospital, LMU Munich, 80337 Munich, GermanyDepartment of Dermatology and Allergy, University Hospital, LMU Munich, 80337 Munich, GermanyDepartment of Dermatology and Allergy, University Hospital, LMU Munich, 80337 Munich, GermanyIt is known that actinic keratoses (AKs) can progress to invasive squamous cell carcinoma (SCC). The histological PRO grading of AKs is based on the growth pattern of basal keratinocytes and relates to their progression risk. AKs can be non-invasively characterized by line-field confocal optical coherence tomography (LC-OCT). The aim of the study was to define criteria for an LC-OCT grading of AKs based on the PRO classification and to correlate it with its histological counterpart. To evaluate the interobserver agreement for the LC-OCT PRO classification, fifty AKs were imaged by LC-OCT and biopsied for histopathology. PRO histological grading was assessed by an expert consensus, while two evaluator groups separately performed LC-OCT grading on vertical sections. The agreement between LC-OCT and histological PRO grading was 75% for all lesions (weighted kappa 0.66, 95% CI 0.48–0.83, <i>p</i> ≤ 0.001) and 85.4% when comparing the subgroups PRO I vs. PRO II/III (weighted kappa 0.64, 95% CI 0.40–0.88, <i>p</i> ≤ 0.001). The interobserver agreement for LC-OCT was 90% (Cohen’s kappa 0.84, 95% CI 0.71–0.91, <i>p</i> ≤ 0.001). In this pilot study, we demonstrated that LC-OCT is potentially able to classify AKs based on the basal growth pattern of keratinocytes, in-vivo reproducing the PRO classification, with strong interobserver agreement and a good correlation with histopathology.https://www.mdpi.com/2072-6694/13/12/2856actinic keratosisPROdysplasiakeratinocyte cancerfield cancerizationline-field confocal optical coherence tomography |
| spellingShingle | Cristel Ruini Sandra Schuh Charlotte Gust Daniela Hartmann Lars Einar French Elke Christina Sattler Julia Welzel In-Vivo LC-OCT Evaluation of the Downward Proliferation Pattern of Keratinocytes in Actinic Keratosis in Comparison with Histology: First Impressions from a Pilot Study Cancers actinic keratosis PRO dysplasia keratinocyte cancer field cancerization line-field confocal optical coherence tomography |
| title | In-Vivo LC-OCT Evaluation of the Downward Proliferation Pattern of Keratinocytes in Actinic Keratosis in Comparison with Histology: First Impressions from a Pilot Study |
| title_full | In-Vivo LC-OCT Evaluation of the Downward Proliferation Pattern of Keratinocytes in Actinic Keratosis in Comparison with Histology: First Impressions from a Pilot Study |
| title_fullStr | In-Vivo LC-OCT Evaluation of the Downward Proliferation Pattern of Keratinocytes in Actinic Keratosis in Comparison with Histology: First Impressions from a Pilot Study |
| title_full_unstemmed | In-Vivo LC-OCT Evaluation of the Downward Proliferation Pattern of Keratinocytes in Actinic Keratosis in Comparison with Histology: First Impressions from a Pilot Study |
| title_short | In-Vivo LC-OCT Evaluation of the Downward Proliferation Pattern of Keratinocytes in Actinic Keratosis in Comparison with Histology: First Impressions from a Pilot Study |
| title_sort | in vivo lc oct evaluation of the downward proliferation pattern of keratinocytes in actinic keratosis in comparison with histology first impressions from a pilot study |
| topic | actinic keratosis PRO dysplasia keratinocyte cancer field cancerization line-field confocal optical coherence tomography |
| url | https://www.mdpi.com/2072-6694/13/12/2856 |
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