Susceptibility of the Gypsy Moth <i>Lymantria dispar</i> (Lepidoptera: Erebidae) to <i>Nosema pyrausta</i> (Microsporidia: Nosematidae)
The gypsy moth, <i>Lymantria dispar</i>, is a notorious forest defoliator, and various pathogens are known to act as natural regulators of its population density. As a widespread herbivore with a broad range of inhabited areas and host plants, it is potentially exposed to parasitic micro...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Insects |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4450/12/5/447 |
| _version_ | 1797534201565675520 |
|---|---|
| author | Anastasia G. Kononchuk Vyacheslav V. Martemyanov Anastasia N. Ignatieva Irina A. Belousova Maki N. Inoue Yuri S. Tokarev |
| author_facet | Anastasia G. Kononchuk Vyacheslav V. Martemyanov Anastasia N. Ignatieva Irina A. Belousova Maki N. Inoue Yuri S. Tokarev |
| author_sort | Anastasia G. Kononchuk |
| collection | DOAJ |
| description | The gypsy moth, <i>Lymantria dispar</i>, is a notorious forest defoliator, and various pathogens are known to act as natural regulators of its population density. As a widespread herbivore with a broad range of inhabited areas and host plants, it is potentially exposed to parasitic microorganisms from other insect hosts. In the present paper, we determined the susceptibility of gypsy moth larvae to the microsporidium <i>Nosema pyrausta</i> from the European corn borer, <i>Ostrinia nubilalis</i>. Gypsy moth samples from two localities of Western Siberia were used. <i>N. pyrausta</i> developed infections in the salivary gland and adipose tissue of gypsy moth prepupae and pupae, forming spore masses after 30 days of alimentary exposure to the second instar larvae. Among the experimental groups, the infection levels ranged from 0 to 9.5%. Effects of a covert baculovirus infection, phenylthiourea pretreatment and feeding insects on an artificial diet versus natural foliage were not significant in terms of microsporidia prevalence levels. Thus, <i>L. dispar</i> showed a low level of susceptibility to a non-specific microsporidium. It can be referred to as a resistant model host and not an appropriate substitute host for laboratory propagation of the microsporidium. |
| first_indexed | 2024-03-10T11:26:17Z |
| format | Article |
| id | doaj.art-dddd2e21a22c44a0b0c130dc7fc0b361 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T11:26:17Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-dddd2e21a22c44a0b0c130dc7fc0b3612023-11-21T19:41:18ZengMDPI AGInsects2075-44502021-05-0112544710.3390/insects12050447Susceptibility of the Gypsy Moth <i>Lymantria dispar</i> (Lepidoptera: Erebidae) to <i>Nosema pyrausta</i> (Microsporidia: Nosematidae)Anastasia G. Kononchuk0Vyacheslav V. Martemyanov1Anastasia N. Ignatieva2Irina A. Belousova3Maki N. Inoue4Yuri S. Tokarev5All-Russian Institute of Plant Protection, Podbelskogo 3, Pushkin, 196608 St. Petersburg, RussiaInstitute of Systematics and Ecology of Animals SB RAS, Frunze 11, 630091 Novosibirsk, RussiaAll-Russian Institute of Plant Protection, Podbelskogo 3, Pushkin, 196608 St. Petersburg, RussiaInstitute of Systematics and Ecology of Animals SB RAS, Frunze 11, 630091 Novosibirsk, RussiaDepartment of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, 3 Chome-8-1 Harumicho, Tokyo 183-8538, JapanAll-Russian Institute of Plant Protection, Podbelskogo 3, Pushkin, 196608 St. Petersburg, RussiaThe gypsy moth, <i>Lymantria dispar</i>, is a notorious forest defoliator, and various pathogens are known to act as natural regulators of its population density. As a widespread herbivore with a broad range of inhabited areas and host plants, it is potentially exposed to parasitic microorganisms from other insect hosts. In the present paper, we determined the susceptibility of gypsy moth larvae to the microsporidium <i>Nosema pyrausta</i> from the European corn borer, <i>Ostrinia nubilalis</i>. Gypsy moth samples from two localities of Western Siberia were used. <i>N. pyrausta</i> developed infections in the salivary gland and adipose tissue of gypsy moth prepupae and pupae, forming spore masses after 30 days of alimentary exposure to the second instar larvae. Among the experimental groups, the infection levels ranged from 0 to 9.5%. Effects of a covert baculovirus infection, phenylthiourea pretreatment and feeding insects on an artificial diet versus natural foliage were not significant in terms of microsporidia prevalence levels. Thus, <i>L. dispar</i> showed a low level of susceptibility to a non-specific microsporidium. It can be referred to as a resistant model host and not an appropriate substitute host for laboratory propagation of the microsporidium.https://www.mdpi.com/2075-4450/12/5/447microsporidiahost rangegypsy mothparasite–host interactionssubstitute host |
| spellingShingle | Anastasia G. Kononchuk Vyacheslav V. Martemyanov Anastasia N. Ignatieva Irina A. Belousova Maki N. Inoue Yuri S. Tokarev Susceptibility of the Gypsy Moth <i>Lymantria dispar</i> (Lepidoptera: Erebidae) to <i>Nosema pyrausta</i> (Microsporidia: Nosematidae) Insects microsporidia host range gypsy moth parasite–host interactions substitute host |
| title | Susceptibility of the Gypsy Moth <i>Lymantria dispar</i> (Lepidoptera: Erebidae) to <i>Nosema pyrausta</i> (Microsporidia: Nosematidae) |
| title_full | Susceptibility of the Gypsy Moth <i>Lymantria dispar</i> (Lepidoptera: Erebidae) to <i>Nosema pyrausta</i> (Microsporidia: Nosematidae) |
| title_fullStr | Susceptibility of the Gypsy Moth <i>Lymantria dispar</i> (Lepidoptera: Erebidae) to <i>Nosema pyrausta</i> (Microsporidia: Nosematidae) |
| title_full_unstemmed | Susceptibility of the Gypsy Moth <i>Lymantria dispar</i> (Lepidoptera: Erebidae) to <i>Nosema pyrausta</i> (Microsporidia: Nosematidae) |
| title_short | Susceptibility of the Gypsy Moth <i>Lymantria dispar</i> (Lepidoptera: Erebidae) to <i>Nosema pyrausta</i> (Microsporidia: Nosematidae) |
| title_sort | susceptibility of the gypsy moth i lymantria dispar i lepidoptera erebidae to i nosema pyrausta i microsporidia nosematidae |
| topic | microsporidia host range gypsy moth parasite–host interactions substitute host |
| url | https://www.mdpi.com/2075-4450/12/5/447 |
| work_keys_str_mv | AT anastasiagkononchuk susceptibilityofthegypsymothilymantriadisparilepidopteraerebidaetoinosemapyraustaimicrosporidianosematidae AT vyacheslavvmartemyanov susceptibilityofthegypsymothilymantriadisparilepidopteraerebidaetoinosemapyraustaimicrosporidianosematidae AT anastasianignatieva susceptibilityofthegypsymothilymantriadisparilepidopteraerebidaetoinosemapyraustaimicrosporidianosematidae AT irinaabelousova susceptibilityofthegypsymothilymantriadisparilepidopteraerebidaetoinosemapyraustaimicrosporidianosematidae AT makininoue susceptibilityofthegypsymothilymantriadisparilepidopteraerebidaetoinosemapyraustaimicrosporidianosematidae AT yuristokarev susceptibilityofthegypsymothilymantriadisparilepidopteraerebidaetoinosemapyraustaimicrosporidianosematidae |