Deconstruction of the (paleo)polyploid grapevine genome based on the analysis of transposition events involving NBS resistance genes.
Plants have followed a reticulate type of evolution and taxa have frequently merged via allopolyploidization. A polyploid structure of sequenced genomes has often been proposed, but the chromosomes belonging to putative component genomes are difficult to identify. The 19 grapevine chromosomes are ev...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3256180?pdf=render |
| _version_ | 1811288694718464000 |
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| author | Giulia Malacarne Michele Perazzolli Alessandro Cestaro Lieven Sterck Paolo Fontana Yves Van de Peer Roberto Viola Riccardo Velasco Francesco Salamini |
| author_facet | Giulia Malacarne Michele Perazzolli Alessandro Cestaro Lieven Sterck Paolo Fontana Yves Van de Peer Roberto Viola Riccardo Velasco Francesco Salamini |
| author_sort | Giulia Malacarne |
| collection | DOAJ |
| description | Plants have followed a reticulate type of evolution and taxa have frequently merged via allopolyploidization. A polyploid structure of sequenced genomes has often been proposed, but the chromosomes belonging to putative component genomes are difficult to identify. The 19 grapevine chromosomes are evolutionary stable structures: their homologous triplets have strongly conserved gene order, interrupted by rare translocations. The aim of this study is to examine how the grapevine nucleotide-binding site (NBS)-encoding resistance (NBS-R) genes have evolved in the genomic context and to understand mechanisms for the genome evolution. We show that, in grapevine, i) helitrons have significantly contributed to transposition of NBS-R genes, and ii) NBS-R gene cluster similarity indicates the existence of two groups of chromosomes (named as Va and Vc) that may have evolved independently. Chromosome triplets consist of two Va and one Vc chromosomes, as expected from the tetraploid and diploid conditions of the two component genomes. The hexaploid state could have been derived from either allopolyploidy or the separation of the Va and Vc component genomes in the same nucleus before fusion, as known for Rosaceae species. Time estimation indicates that grapevine component genomes may have fused about 60 mya, having had at least 40-60 mya to evolve independently. Chromosome number variation in the Vitaceae and related families, and the gap between the time of eudicot radiation and the age of Vitaceae fossils, are accounted for by our hypothesis. |
| first_indexed | 2024-04-13T03:41:49Z |
| format | Article |
| id | doaj.art-dddee3cb919542e5990b5b7ca20f9f35 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T03:41:49Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-dddee3cb919542e5990b5b7ca20f9f352022-12-22T03:04:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0171e2976210.1371/journal.pone.0029762Deconstruction of the (paleo)polyploid grapevine genome based on the analysis of transposition events involving NBS resistance genes.Giulia MalacarneMichele PerazzolliAlessandro CestaroLieven SterckPaolo FontanaYves Van de PeerRoberto ViolaRiccardo VelascoFrancesco SalaminiPlants have followed a reticulate type of evolution and taxa have frequently merged via allopolyploidization. A polyploid structure of sequenced genomes has often been proposed, but the chromosomes belonging to putative component genomes are difficult to identify. The 19 grapevine chromosomes are evolutionary stable structures: their homologous triplets have strongly conserved gene order, interrupted by rare translocations. The aim of this study is to examine how the grapevine nucleotide-binding site (NBS)-encoding resistance (NBS-R) genes have evolved in the genomic context and to understand mechanisms for the genome evolution. We show that, in grapevine, i) helitrons have significantly contributed to transposition of NBS-R genes, and ii) NBS-R gene cluster similarity indicates the existence of two groups of chromosomes (named as Va and Vc) that may have evolved independently. Chromosome triplets consist of two Va and one Vc chromosomes, as expected from the tetraploid and diploid conditions of the two component genomes. The hexaploid state could have been derived from either allopolyploidy or the separation of the Va and Vc component genomes in the same nucleus before fusion, as known for Rosaceae species. Time estimation indicates that grapevine component genomes may have fused about 60 mya, having had at least 40-60 mya to evolve independently. Chromosome number variation in the Vitaceae and related families, and the gap between the time of eudicot radiation and the age of Vitaceae fossils, are accounted for by our hypothesis.http://europepmc.org/articles/PMC3256180?pdf=render |
| spellingShingle | Giulia Malacarne Michele Perazzolli Alessandro Cestaro Lieven Sterck Paolo Fontana Yves Van de Peer Roberto Viola Riccardo Velasco Francesco Salamini Deconstruction of the (paleo)polyploid grapevine genome based on the analysis of transposition events involving NBS resistance genes. PLoS ONE |
| title | Deconstruction of the (paleo)polyploid grapevine genome based on the analysis of transposition events involving NBS resistance genes. |
| title_full | Deconstruction of the (paleo)polyploid grapevine genome based on the analysis of transposition events involving NBS resistance genes. |
| title_fullStr | Deconstruction of the (paleo)polyploid grapevine genome based on the analysis of transposition events involving NBS resistance genes. |
| title_full_unstemmed | Deconstruction of the (paleo)polyploid grapevine genome based on the analysis of transposition events involving NBS resistance genes. |
| title_short | Deconstruction of the (paleo)polyploid grapevine genome based on the analysis of transposition events involving NBS resistance genes. |
| title_sort | deconstruction of the paleo polyploid grapevine genome based on the analysis of transposition events involving nbs resistance genes |
| url | http://europepmc.org/articles/PMC3256180?pdf=render |
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