Clonal Micropropagation of <i>Cymbidium erythrostylum</i> Rolfe
<i>Cymbidium erythrostylum</i> Rolfe is one of the most beautiful species of the genus cymbidium which is used as a cut flower or indoor plant. However, it was registered as a rare species even in its original habitat. This study was carried out to develop a protocol for in vitro propaga...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Plant Biology |
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| Online Access: | https://www.mdpi.com/2037-0164/14/1/3 |
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| author | Muthab Hussien Viktoriya Kryuchkova Ekaterina Raeva-Bogoslovskaya Olga Molkanova |
| author_facet | Muthab Hussien Viktoriya Kryuchkova Ekaterina Raeva-Bogoslovskaya Olga Molkanova |
| author_sort | Muthab Hussien |
| collection | DOAJ |
| description | <i>Cymbidium erythrostylum</i> Rolfe is one of the most beautiful species of the genus cymbidium which is used as a cut flower or indoor plant. However, it was registered as a rare species even in its original habitat. This study was carried out to develop a protocol for in vitro propagation of <i>C. erythrostilum</i>. We used protosomes obtained by the asymbiotic germination of seed on 1/2 of Murashige and Skoog nutrient medium (MS) supplemented with 1 mg/L 2-Isopentenyl adenine (2iP) as objects of study. During the multiplication stage, the number of formed protosomes on a culture medium containing 0.8 mg/L 6-Benzylaminopurine (6-BAP) was higher (3.8 ± 0.2 protosomes). During the subsequent cultivation on ½ MS medium supplemented with 2 mg/L 6-BAP and 0.5 mg/L a-naphthaleneacetic acid (NAA), the highest numbers of shoots (4.00 ± 0.19 shoots/plantlet) and leaves (4.50 ± 0.14 leaves/plantlet) were obtained. At the rooting stage under in vitro conditions, the most effective was the use of ½ MS nutrient medium with the addition of 0.5 mg/L of indolyl-3-butyric acid IBA, 1 g/l charcoal, and 50 g/l banana puree. The obtained plants were successfully adapted to a substrate consisting of bark, perlite, and peat in a ratio of 1:1:1. |
| first_indexed | 2024-03-11T06:24:30Z |
| format | Article |
| id | doaj.art-dddfaa2a51944d078c3a476dedcd1bf4 |
| institution | Directory Open Access Journal |
| issn | 2037-0164 |
| language | English |
| last_indexed | 2024-03-11T06:24:30Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Plant Biology |
| spelling | doaj.art-dddfaa2a51944d078c3a476dedcd1bf42023-11-17T11:42:32ZengMDPI AGInternational Journal of Plant Biology2037-01642022-12-01141283810.3390/ijpb14010003Clonal Micropropagation of <i>Cymbidium erythrostylum</i> RolfeMuthab Hussien0Viktoriya Kryuchkova1Ekaterina Raeva-Bogoslovskaya2Olga Molkanova3Institute of Horticulture and Landscape Architecture, Russian State Agrarian University—Moscow Timiryazev Agricultural Academy, 127550 Moscow, RussiaTsitsin Main Botanical Garden of Russian Academy of Science, 127276 Moscow, RussiaTsitsin Main Botanical Garden of Russian Academy of Science, 127276 Moscow, RussiaTsitsin Main Botanical Garden of Russian Academy of Science, 127276 Moscow, Russia<i>Cymbidium erythrostylum</i> Rolfe is one of the most beautiful species of the genus cymbidium which is used as a cut flower or indoor plant. However, it was registered as a rare species even in its original habitat. This study was carried out to develop a protocol for in vitro propagation of <i>C. erythrostilum</i>. We used protosomes obtained by the asymbiotic germination of seed on 1/2 of Murashige and Skoog nutrient medium (MS) supplemented with 1 mg/L 2-Isopentenyl adenine (2iP) as objects of study. During the multiplication stage, the number of formed protosomes on a culture medium containing 0.8 mg/L 6-Benzylaminopurine (6-BAP) was higher (3.8 ± 0.2 protosomes). During the subsequent cultivation on ½ MS medium supplemented with 2 mg/L 6-BAP and 0.5 mg/L a-naphthaleneacetic acid (NAA), the highest numbers of shoots (4.00 ± 0.19 shoots/plantlet) and leaves (4.50 ± 0.14 leaves/plantlet) were obtained. At the rooting stage under in vitro conditions, the most effective was the use of ½ MS nutrient medium with the addition of 0.5 mg/L of indolyl-3-butyric acid IBA, 1 g/l charcoal, and 50 g/l banana puree. The obtained plants were successfully adapted to a substrate consisting of bark, perlite, and peat in a ratio of 1:1:1.https://www.mdpi.com/2037-0164/14/1/3orchidspropagationindoor plantsgrowth regulatorscymbidiumin vitro |
| spellingShingle | Muthab Hussien Viktoriya Kryuchkova Ekaterina Raeva-Bogoslovskaya Olga Molkanova Clonal Micropropagation of <i>Cymbidium erythrostylum</i> Rolfe International Journal of Plant Biology orchids propagation indoor plants growth regulators cymbidium in vitro |
| title | Clonal Micropropagation of <i>Cymbidium erythrostylum</i> Rolfe |
| title_full | Clonal Micropropagation of <i>Cymbidium erythrostylum</i> Rolfe |
| title_fullStr | Clonal Micropropagation of <i>Cymbidium erythrostylum</i> Rolfe |
| title_full_unstemmed | Clonal Micropropagation of <i>Cymbidium erythrostylum</i> Rolfe |
| title_short | Clonal Micropropagation of <i>Cymbidium erythrostylum</i> Rolfe |
| title_sort | clonal micropropagation of i cymbidium erythrostylum i rolfe |
| topic | orchids propagation indoor plants growth regulators cymbidium in vitro |
| url | https://www.mdpi.com/2037-0164/14/1/3 |
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