The Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological Study
Background: Co-administration of two or several either chemotherapeutic agents or conventional drugs as a combination treatment is the most effective method to increase therapeutic efficiency. Additive or synergistic influence are two mechanisms by which combination therapy causes a rise in optimal...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2021-12-01
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| Series: | Pharmaceutical Sciences |
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| Online Access: | https://ps.tbzmed.ac.ir/PDF/ps-27-536.pdf |
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| author | Hossein Majidzadeh Mostafa Araj-Khodaei Maryam Ghaffari Amir Jafari Dariush Shanehbandi Mohammadali Torbati Jafar Ezzati Nazhad Dolatabadi |
| author_facet | Hossein Majidzadeh Mostafa Araj-Khodaei Maryam Ghaffari Amir Jafari Dariush Shanehbandi Mohammadali Torbati Jafar Ezzati Nazhad Dolatabadi |
| author_sort | Hossein Majidzadeh |
| collection | DOAJ |
| description | Background: Co-administration of two or several either chemotherapeutic agents or conventional drugs as a combination treatment is the most effective method to increase therapeutic efficiency. Additive or synergistic influence are two mechanisms by which combination therapy causes a rise in optimal cancer therapy compared to a single treatment. Methods: Colorimetric assay was carried out to estimate the cytotoxicity of the combined system, followed by apoptosis assay to calculate the number of apoptotic cells. Both 4′,6-diamidino-2-phenylindole (DAPI) staining and DNA ladder were complemented tests to illuminate morphological changes and DNA fracturing on HeLa cancer cells. Results: We revealed that the combination of berberine (BER) and methotrexate (MTX) could inhibit the growth of HeLa cancer cells noticeably. Nevertheless, single BER and MTX were not as effective as a combined system to reduce cell viability at the same dose. Regarding the apoptosis induction and change in morphology of cancer cells’ nucleus, co-treatment of BERand MTX was more effective. The result was complemented with flow cytometry, DAPI staining and DNA ladder, which showed that BER+MTX depicted more anti-cancer effects. Conclusion: The combination therapy of HeLa cancer cells with BER and MTX showed high inhibition effect compared to other treated groups. |
| first_indexed | 2024-12-19T04:14:43Z |
| format | Article |
| id | doaj.art-dde0705bc4b14d4b882963ec9d3e323b |
| institution | Directory Open Access Journal |
| issn | 2383-2886 |
| language | English |
| last_indexed | 2024-12-19T04:14:43Z |
| publishDate | 2021-12-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Pharmaceutical Sciences |
| spelling | doaj.art-dde0705bc4b14d4b882963ec9d3e323b2022-12-21T20:36:19ZengTabriz University of Medical SciencesPharmaceutical Sciences2383-28862021-12-0127453654210.34172/PS.2021.12ps-34135The Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological StudyHossein Majidzadeh0Mostafa Araj-Khodaei1Maryam Ghaffari2Amir Jafari3Dariush Shanehbandi4Mohammadali Torbati5Jafar Ezzati Nazhad Dolatabadi6Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Persian Medicine, School of Traditional Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Laboratory of Nanoradiopharmaceuticals and Synthesis of Novel Radiopharmaceuticals, Nuclear Engineering Institute, Brazilian Nuclear Energy Commission, Rio de Janeiro, Brazil.Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Department of Persian Medicine, School of Traditional Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.Background: Co-administration of two or several either chemotherapeutic agents or conventional drugs as a combination treatment is the most effective method to increase therapeutic efficiency. Additive or synergistic influence are two mechanisms by which combination therapy causes a rise in optimal cancer therapy compared to a single treatment. Methods: Colorimetric assay was carried out to estimate the cytotoxicity of the combined system, followed by apoptosis assay to calculate the number of apoptotic cells. Both 4′,6-diamidino-2-phenylindole (DAPI) staining and DNA ladder were complemented tests to illuminate morphological changes and DNA fracturing on HeLa cancer cells. Results: We revealed that the combination of berberine (BER) and methotrexate (MTX) could inhibit the growth of HeLa cancer cells noticeably. Nevertheless, single BER and MTX were not as effective as a combined system to reduce cell viability at the same dose. Regarding the apoptosis induction and change in morphology of cancer cells’ nucleus, co-treatment of BERand MTX was more effective. The result was complemented with flow cytometry, DAPI staining and DNA ladder, which showed that BER+MTX depicted more anti-cancer effects. Conclusion: The combination therapy of HeLa cancer cells with BER and MTX showed high inhibition effect compared to other treated groups.https://ps.tbzmed.ac.ir/PDF/ps-27-536.pdfhela cellsmethotrexateberberineherbal medicinenuclear morphology |
| spellingShingle | Hossein Majidzadeh Mostafa Araj-Khodaei Maryam Ghaffari Amir Jafari Dariush Shanehbandi Mohammadali Torbati Jafar Ezzati Nazhad Dolatabadi The Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological Study Pharmaceutical Sciences hela cells methotrexate berberine herbal medicine nuclear morphology |
| title | The Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological Study |
| title_full | The Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological Study |
| title_fullStr | The Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological Study |
| title_full_unstemmed | The Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological Study |
| title_short | The Combination of Berberine and Methotrexate Enhances Anti-Cancer Effects in Hela Cancer Cell Line: A Morphological Study |
| title_sort | combination of berberine and methotrexate enhances anti cancer effects in hela cancer cell line a morphological study |
| topic | hela cells methotrexate berberine herbal medicine nuclear morphology |
| url | https://ps.tbzmed.ac.ir/PDF/ps-27-536.pdf |
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