Comparative Phytochemical Characterization, Genetic Profile, and Antiproliferative Activity of Polyphenol-Rich Extracts from Pigmented Tubers of Different <i>Solanum tuberosum</i> Varieties
Plants produce a vast array of biomolecules with beneficial effects for human health. In this study, polyphenol and anthocyanin-rich extracts (PAE) from pigmented tubers of <i>Solanum tuberosum</i> L. varieties “Blue Star”, “Magenta Love”, and &...
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MDPI AG
2020-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/1/233 |
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| author | Luigi De Masi Paola Bontempo Daniela Rigano Paola Stiuso Vincenzo Carafa Angela Nebbioso Sonia Piacente Paola Montoro Riccardo Aversano Vincenzo D’Amelia Domenico Carputo Lucia Altucci |
| author_facet | Luigi De Masi Paola Bontempo Daniela Rigano Paola Stiuso Vincenzo Carafa Angela Nebbioso Sonia Piacente Paola Montoro Riccardo Aversano Vincenzo D’Amelia Domenico Carputo Lucia Altucci |
| author_sort | Luigi De Masi |
| collection | DOAJ |
| description | Plants produce a vast array of biomolecules with beneficial effects for human health. In this study, polyphenol and anthocyanin-rich extracts (PAE) from pigmented tubers of <i>Solanum tuberosum</i> L. varieties “Blue Star”, “Magenta Love”, and “Double Fun” in comparison with the more extensively studied “Vitelotte” were evaluated and compared for antiproliferative effects in human leukemia cells, and their phytochemical and genetic profiles were determined. In U937 cells, upon treatment with PAE, it was possible to reveal the expression of specific apoptotic players, such as caspase 8, 9, 3, and poly (ADP-ribose) polymerase (PARP), as well as the induction of monocyte and granulocyte differentiation. A liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) investigation revealed the presence of polyphenolic compounds in all the varieties of potatoes analyzed, among which caffeoyl and feruloyl quinic acid derivatives were the most abundant, as well as several acylated anthocyanins. Each pigmented variety was genotyped by DNA-based molecular markers, and flavonoid-related transcription factors were profiled in tubers in order to better characterize these outstanding resources and contribute to their exploitation in breeding. Interesting biological activities were observed for “Blue Star” and “Vitelotte” varieties with respect to the minor or no effect of the “Double Fun” variety. |
| first_indexed | 2024-12-11T09:55:12Z |
| format | Article |
| id | doaj.art-dde1ab2dea5e4096be568929f99882f9 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T09:55:12Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-dde1ab2dea5e4096be568929f99882f92022-12-22T01:12:17ZengMDPI AGMolecules1420-30492020-01-0125123310.3390/molecules25010233molecules25010233Comparative Phytochemical Characterization, Genetic Profile, and Antiproliferative Activity of Polyphenol-Rich Extracts from Pigmented Tubers of Different <i>Solanum tuberosum</i> VarietiesLuigi De Masi0Paola Bontempo1Daniela Rigano2Paola Stiuso3Vincenzo Carafa4Angela Nebbioso5Sonia Piacente6Paola Montoro7Riccardo Aversano8Vincenzo D’Amelia9Domenico Carputo10Lucia Altucci11National Research Council (CNR), Institute of Biosciences and Bioresources (IBBR), Via Università 133, 80055 Portici (Naples), ItalyDepartment of Precision Medicine, University of Campania “Luigi Vanvitelli”, Via L. De Crecchio 7, 80138 Naples, ItalyDepartment of Pharmacy, University of Naples Federico II, Via Domenico Montesano 49, 80131 Naples, ItalyDepartment of Precision Medicine, University of Campania “Luigi Vanvitelli”, Via L. De Crecchio 7, 80138 Naples, ItalyDepartment of Precision Medicine, University of Campania “Luigi Vanvitelli”, Via L. De Crecchio 7, 80138 Naples, ItalyDepartment of Precision Medicine, University of Campania “Luigi Vanvitelli”, Via L. De Crecchio 7, 80138 Naples, ItalyDepartment of Pharmacy, University of Salerno, via Giovanni Paolo II 132, 84084 Fisciano (Salerno), ItalyDepartment of Pharmacy, University of Salerno, via Giovanni Paolo II 132, 84084 Fisciano (Salerno), ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055 Portici (Naples), ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055 Portici (Naples), ItalyDepartment of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055 Portici (Naples), ItalyDepartment of Precision Medicine, University of Campania “Luigi Vanvitelli”, Via L. De Crecchio 7, 80138 Naples, ItalyPlants produce a vast array of biomolecules with beneficial effects for human health. In this study, polyphenol and anthocyanin-rich extracts (PAE) from pigmented tubers of <i>Solanum tuberosum</i> L. varieties “Blue Star”, “Magenta Love”, and “Double Fun” in comparison with the more extensively studied “Vitelotte” were evaluated and compared for antiproliferative effects in human leukemia cells, and their phytochemical and genetic profiles were determined. In U937 cells, upon treatment with PAE, it was possible to reveal the expression of specific apoptotic players, such as caspase 8, 9, 3, and poly (ADP-ribose) polymerase (PARP), as well as the induction of monocyte and granulocyte differentiation. A liquid chromatography/electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) investigation revealed the presence of polyphenolic compounds in all the varieties of potatoes analyzed, among which caffeoyl and feruloyl quinic acid derivatives were the most abundant, as well as several acylated anthocyanins. Each pigmented variety was genotyped by DNA-based molecular markers, and flavonoid-related transcription factors were profiled in tubers in order to better characterize these outstanding resources and contribute to their exploitation in breeding. Interesting biological activities were observed for “Blue Star” and “Vitelotte” varieties with respect to the minor or no effect of the “Double Fun” variety.https://www.mdpi.com/1420-3049/25/1/233pigmented potatoespotato tuber extractleukemia cellscell cycle modulationbioactive moleculespotato genotyping |
| spellingShingle | Luigi De Masi Paola Bontempo Daniela Rigano Paola Stiuso Vincenzo Carafa Angela Nebbioso Sonia Piacente Paola Montoro Riccardo Aversano Vincenzo D’Amelia Domenico Carputo Lucia Altucci Comparative Phytochemical Characterization, Genetic Profile, and Antiproliferative Activity of Polyphenol-Rich Extracts from Pigmented Tubers of Different <i>Solanum tuberosum</i> Varieties Molecules pigmented potatoes potato tuber extract leukemia cells cell cycle modulation bioactive molecules potato genotyping |
| title | Comparative Phytochemical Characterization, Genetic Profile, and Antiproliferative Activity of Polyphenol-Rich Extracts from Pigmented Tubers of Different <i>Solanum tuberosum</i> Varieties |
| title_full | Comparative Phytochemical Characterization, Genetic Profile, and Antiproliferative Activity of Polyphenol-Rich Extracts from Pigmented Tubers of Different <i>Solanum tuberosum</i> Varieties |
| title_fullStr | Comparative Phytochemical Characterization, Genetic Profile, and Antiproliferative Activity of Polyphenol-Rich Extracts from Pigmented Tubers of Different <i>Solanum tuberosum</i> Varieties |
| title_full_unstemmed | Comparative Phytochemical Characterization, Genetic Profile, and Antiproliferative Activity of Polyphenol-Rich Extracts from Pigmented Tubers of Different <i>Solanum tuberosum</i> Varieties |
| title_short | Comparative Phytochemical Characterization, Genetic Profile, and Antiproliferative Activity of Polyphenol-Rich Extracts from Pigmented Tubers of Different <i>Solanum tuberosum</i> Varieties |
| title_sort | comparative phytochemical characterization genetic profile and antiproliferative activity of polyphenol rich extracts from pigmented tubers of different i solanum tuberosum i varieties |
| topic | pigmented potatoes potato tuber extract leukemia cells cell cycle modulation bioactive molecules potato genotyping |
| url | https://www.mdpi.com/1420-3049/25/1/233 |
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