Transcriptomic profiling of hepatic tissues for drug metabolism genes in nonalcoholic fatty liver disease: A study of human and animals
BackgroundDrug metabolism genes are involved in the in vivo metabolic processing of drugs. In previous research, we found that a high-fat diet affected the transcript levels of mouse hepatic genes responsible for drug metabolism.AimsOur research intends to discover the drug metabolism genes that are...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.1034494/full |
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| author | Li Chen Li Chen Li Chen Lu Chen Lu Chen Xu Li Xu Li Lin Qin Lin Qin Yan Zhu Qianru Zhang Qianru Zhang Daopeng Tan Daopeng Tan Yuqi He Yuqi He Yu-He Wang Yu-He Wang Yu-He Wang |
| author_facet | Li Chen Li Chen Li Chen Lu Chen Lu Chen Xu Li Xu Li Lin Qin Lin Qin Yan Zhu Qianru Zhang Qianru Zhang Daopeng Tan Daopeng Tan Yuqi He Yuqi He Yu-He Wang Yu-He Wang Yu-He Wang |
| author_sort | Li Chen |
| collection | DOAJ |
| description | BackgroundDrug metabolism genes are involved in the in vivo metabolic processing of drugs. In previous research, we found that a high-fat diet affected the transcript levels of mouse hepatic genes responsible for drug metabolism.AimsOur research intends to discover the drug metabolism genes that are dysregulated at the transcriptome level in nonalcoholic fatty liver disease (NAFLD).MethodsWe analyzed the transcriptome for drug metabolism genes of 35 human liver tissues obtained during laparoscopic cholecystectomy. Additionally, we imported transcriptome data from mice fed a high-fat diet in previous research and two open-access Gene Expression Omnibus (GEO) datasets (GSE63067 and GSE89632). Then, using quantitative real-time polymerase chain reaction (qRT-PCR), we cross-linked the differentially expressed genes (DEGs) in clinical and animal samples and validated the common genes.ResultsIn this study, we identified 35 DEGs, of which 33 were up-regulated and two were down-regulated. Moreover, we found 71 DEGs (39 up- and 32 down-regulated), 276 DEGs (157 up- and 119 down-regulated), and 158 DEGs (117 up- and 41 down-regulated) in the GSE63067, GSE89632, and high-fat diet mice, respectively. Of the 35 DEGs, nine co-regulated DEGs were found in the Venn diagram (CYP20A1, CYP2U1, SLC9A6, SLC26A6, SLC31A1, SLC46A1, SLC46A3, SULT1B1, and UGT2A3).ConclusionNine significant drug metabolism genes were identified in NAFLD. Future research should investigate the impacts of these genes on drug dose adjustment in patients with NAFLD.Clinical Trial Registrationhttp://www.chictr.org.cn, identifier ChiCTR2100041714. |
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| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-11T01:06:57Z |
| publishDate | 2023-01-01 |
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| series | Frontiers in Endocrinology |
| spelling | doaj.art-dde4ba40228b42fb84c09674ca96229d2023-01-04T12:12:37ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922023-01-011310.3389/fendo.2022.10344941034494Transcriptomic profiling of hepatic tissues for drug metabolism genes in nonalcoholic fatty liver disease: A study of human and animalsLi Chen0Li Chen1Li Chen2Lu Chen3Lu Chen4Xu Li5Xu Li6Lin Qin7Lin Qin8Yan Zhu9Qianru Zhang10Qianru Zhang11Daopeng Tan12Daopeng Tan13Yuqi He14Yuqi He15Yu-He Wang16Yu-He Wang17Yu-He Wang18Department of Pharmacy, Affiliated Hospital of Zunyi Medical University, Zunyi, ChinaThe Key Laboratory of the Ministry of Education of the Basic Pharmacology, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaThe Joint International Research Laboratory of Ethnomedicine of the Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaThe Key Laboratory of the Ministry of Education of the Basic Pharmacology, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaThe Joint International Research Laboratory of Ethnomedicine of the Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaThe Key Laboratory of the Ministry of Education of the Basic Pharmacology, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaThe Joint International Research Laboratory of Ethnomedicine of the Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaThe Key Laboratory of the Ministry of Education of the Basic Pharmacology, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaThe Joint International Research Laboratory of Ethnomedicine of the Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaDepartment of Pharmacy, Affiliated Hospital of Zunyi Medical University, Zunyi, ChinaThe Key Laboratory of the Ministry of Education of the Basic Pharmacology, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaThe Joint International Research Laboratory of Ethnomedicine of the Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaThe Key Laboratory of the Ministry of Education of the Basic Pharmacology, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaThe Joint International Research Laboratory of Ethnomedicine of the Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaThe Key Laboratory of the Ministry of Education of the Basic Pharmacology, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaThe Joint International Research Laboratory of Ethnomedicine of the Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaDepartment of Pharmacy, Affiliated Hospital of Zunyi Medical University, Zunyi, ChinaThe Key Laboratory of the Ministry of Education of the Basic Pharmacology, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaThe Joint International Research Laboratory of Ethnomedicine of the Ministry of Education, School of Pharmacy, Zunyi Medical University, Zunyi, ChinaBackgroundDrug metabolism genes are involved in the in vivo metabolic processing of drugs. In previous research, we found that a high-fat diet affected the transcript levels of mouse hepatic genes responsible for drug metabolism.AimsOur research intends to discover the drug metabolism genes that are dysregulated at the transcriptome level in nonalcoholic fatty liver disease (NAFLD).MethodsWe analyzed the transcriptome for drug metabolism genes of 35 human liver tissues obtained during laparoscopic cholecystectomy. Additionally, we imported transcriptome data from mice fed a high-fat diet in previous research and two open-access Gene Expression Omnibus (GEO) datasets (GSE63067 and GSE89632). Then, using quantitative real-time polymerase chain reaction (qRT-PCR), we cross-linked the differentially expressed genes (DEGs) in clinical and animal samples and validated the common genes.ResultsIn this study, we identified 35 DEGs, of which 33 were up-regulated and two were down-regulated. Moreover, we found 71 DEGs (39 up- and 32 down-regulated), 276 DEGs (157 up- and 119 down-regulated), and 158 DEGs (117 up- and 41 down-regulated) in the GSE63067, GSE89632, and high-fat diet mice, respectively. Of the 35 DEGs, nine co-regulated DEGs were found in the Venn diagram (CYP20A1, CYP2U1, SLC9A6, SLC26A6, SLC31A1, SLC46A1, SLC46A3, SULT1B1, and UGT2A3).ConclusionNine significant drug metabolism genes were identified in NAFLD. Future research should investigate the impacts of these genes on drug dose adjustment in patients with NAFLD.Clinical Trial Registrationhttp://www.chictr.org.cn, identifier ChiCTR2100041714.https://www.frontiersin.org/articles/10.3389/fendo.2022.1034494/fullnonalcoholic fatty liver diseasetranscriptomedrug metabolism genesGEO datasetshigh-fat diet |
| spellingShingle | Li Chen Li Chen Li Chen Lu Chen Lu Chen Xu Li Xu Li Lin Qin Lin Qin Yan Zhu Qianru Zhang Qianru Zhang Daopeng Tan Daopeng Tan Yuqi He Yuqi He Yu-He Wang Yu-He Wang Yu-He Wang Transcriptomic profiling of hepatic tissues for drug metabolism genes in nonalcoholic fatty liver disease: A study of human and animals Frontiers in Endocrinology nonalcoholic fatty liver disease transcriptome drug metabolism genes GEO datasets high-fat diet |
| title | Transcriptomic profiling of hepatic tissues for drug metabolism genes in nonalcoholic fatty liver disease: A study of human and animals |
| title_full | Transcriptomic profiling of hepatic tissues for drug metabolism genes in nonalcoholic fatty liver disease: A study of human and animals |
| title_fullStr | Transcriptomic profiling of hepatic tissues for drug metabolism genes in nonalcoholic fatty liver disease: A study of human and animals |
| title_full_unstemmed | Transcriptomic profiling of hepatic tissues for drug metabolism genes in nonalcoholic fatty liver disease: A study of human and animals |
| title_short | Transcriptomic profiling of hepatic tissues for drug metabolism genes in nonalcoholic fatty liver disease: A study of human and animals |
| title_sort | transcriptomic profiling of hepatic tissues for drug metabolism genes in nonalcoholic fatty liver disease a study of human and animals |
| topic | nonalcoholic fatty liver disease transcriptome drug metabolism genes GEO datasets high-fat diet |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.1034494/full |
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