Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP
During in vitro propagation, cytokinins (CKs) and auxins (AUXs), such as 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA), are often used to induce adventitious shoots and roots, respectively. However, it is not clear how CKs affect plants over a long period of in vitro propagation as w...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Floricultura e Plantas Ornamentais
2020-06-01
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| Series: | Ornamental Horticulture |
| Subjects: | |
| Online Access: | https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/2138 |
| _version_ | 1797797498433044480 |
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| author | João Paulo Rodrigues Martins Luiz Carlos de Almeida Rodrigues Thayna dos Santos Silva Andreia Barcelos Passos Lima Gontijo Antelmo Ralph Falqueto |
| author_facet | João Paulo Rodrigues Martins Luiz Carlos de Almeida Rodrigues Thayna dos Santos Silva Andreia Barcelos Passos Lima Gontijo Antelmo Ralph Falqueto |
| author_sort | João Paulo Rodrigues Martins |
| collection | DOAJ |
| description |
During in vitro propagation, cytokinins (CKs) and auxins (AUXs), such as 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA), are often used to induce adventitious shoots and roots, respectively. However, it is not clear how CKs affect plants over a long period of in vitro propagation as well as the synergy of direct exposure to AUX with previous CK treatments. The aim was to assess the physiological and anatomical responses of Alcantarea imperialis in function of the interaction of both previous BAP treatments and direct NAA exposure during in vitro propagation. Plants previously grown in vitro were transferred to media containing 0, 5, 10 or 15 μM BAP. After 60 days, the adventitious shoots from each previous BAP treatment were subcultured in media with 0, 2 or 4 μM NAA. Pigment content, anatomical and growth traits were assessed in the plants from each treatment. Both previous BAP treatments and direct NAA exposure altered the anatomy and pigment contents of plants as well as their growth traits. BAP induced negative effects over the long term on physiological status as well as changed the plants’ anatomy. NAA supplementation in the medium can partially reverse the negative effects induced by BAP. The application of 2 μM NAA during in vitro rooting improved the plants’ quality
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| first_indexed | 2024-03-13T03:49:17Z |
| format | Article |
| id | doaj.art-dde5dd4e60ff4058921bd744d43f412e |
| institution | Directory Open Access Journal |
| issn | 2447-536X |
| language | English |
| last_indexed | 2024-03-13T03:49:17Z |
| publishDate | 2020-06-01 |
| publisher | Sociedade Brasileira de Floricultura e Plantas Ornamentais |
| record_format | Article |
| series | Ornamental Horticulture |
| spelling | doaj.art-dde5dd4e60ff4058921bd744d43f412e2023-06-22T20:34:40ZengSociedade Brasileira de Floricultura e Plantas OrnamentaisOrnamental Horticulture2447-536X2020-06-0126210.1590/2447-536X.v26i2.21382105Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAPJoão Paulo Rodrigues Martins0Luiz Carlos de Almeida Rodrigues1Thayna dos Santos Silva2Andreia Barcelos Passos Lima Gontijo3Antelmo Ralph Falqueto4Federal University of Espírito Santo (UFES)Federal University of Alfenas (UNIFAL-MG)Federal University of Espírito Santo (UFES)Federal University of Espírito Santo (UFES)Federal University of Espírito Santo (UFES) During in vitro propagation, cytokinins (CKs) and auxins (AUXs), such as 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA), are often used to induce adventitious shoots and roots, respectively. However, it is not clear how CKs affect plants over a long period of in vitro propagation as well as the synergy of direct exposure to AUX with previous CK treatments. The aim was to assess the physiological and anatomical responses of Alcantarea imperialis in function of the interaction of both previous BAP treatments and direct NAA exposure during in vitro propagation. Plants previously grown in vitro were transferred to media containing 0, 5, 10 or 15 μM BAP. After 60 days, the adventitious shoots from each previous BAP treatment were subcultured in media with 0, 2 or 4 μM NAA. Pigment content, anatomical and growth traits were assessed in the plants from each treatment. Both previous BAP treatments and direct NAA exposure altered the anatomy and pigment contents of plants as well as their growth traits. BAP induced negative effects over the long term on physiological status as well as changed the plants’ anatomy. NAA supplementation in the medium can partially reverse the negative effects induced by BAP. The application of 2 μM NAA during in vitro rooting improved the plants’ quality https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/2138bromeliadauxincytokininphysiological disordersplant anatomy. |
| spellingShingle | João Paulo Rodrigues Martins Luiz Carlos de Almeida Rodrigues Thayna dos Santos Silva Andreia Barcelos Passos Lima Gontijo Antelmo Ralph Falqueto Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP Ornamental Horticulture bromeliad auxin cytokinin physiological disorders plant anatomy. |
| title | Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP |
| title_full | Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP |
| title_fullStr | Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP |
| title_full_unstemmed | Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP |
| title_short | Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP |
| title_sort | modulation of the anatomical and physiological responses of in vitro grown alcantarea imperialis induced by naa and residual effects of bap |
| topic | bromeliad auxin cytokinin physiological disorders plant anatomy. |
| url | https://ornamentalhorticulture.emnuvens.com.br/rbho/article/view/2138 |
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