Adaptive Fed-batch Control of Escherichia coli Fermentation for Protein Production
A new adaptive linearizing control algorithm that stabilizes the carbon source concentration in a desired value is proposed. This algorithm is applied to recombinant protein production by Escherichia coli. A model for control of the investigated process is derived. The model identification is made b...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Bulgarian Academy of Sciences
2023-09-01
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| Series: | International Journal Bioautomation |
| Subjects: | |
| Online Access: | http://www.biomed.bas.bg/bioautomation/2023/vol_27.3/files/27.3_03.pdf |
| _version_ | 1827804115990740992 |
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| author | Velislava Lyubenova Anastasiya Zlatkova Maya Ignatova |
| author_facet | Velislava Lyubenova Anastasiya Zlatkova Maya Ignatova |
| author_sort | Velislava Lyubenova |
| collection | DOAJ |
| description | A new adaptive linearizing control algorithm that stabilizes the carbon source concentration in a desired value is proposed. This algorithm is applied to recombinant protein production by Escherichia coli. A model for control of the investigated process is derived. The model identification is made based on experimental data of the batch phase of the process. The operating model includes two sub-models. Each of them describes one of the two physiological states through which the process passes. Switching from one model to another depends on the sign of a key parameter obtained from the acetate measurements. A cascade scheme of software sensors for the estimation of two biomass growth rates included in the structure of the proposed control algorithm is derived. Simulation studies of the developed closed system have been carried out. The results of the impact of an open-loop control system on the same object are compared. |
| first_indexed | 2024-03-11T21:06:21Z |
| format | Article |
| id | doaj.art-dde9af7434d149c3b255fff1e65c59a8 |
| institution | Directory Open Access Journal |
| issn | 1314-1902 1314-2321 |
| language | English |
| last_indexed | 2024-03-11T21:06:21Z |
| publishDate | 2023-09-01 |
| publisher | Bulgarian Academy of Sciences |
| record_format | Article |
| series | International Journal Bioautomation |
| spelling | doaj.art-dde9af7434d149c3b255fff1e65c59a82023-09-29T13:29:44ZengBulgarian Academy of SciencesInternational Journal Bioautomation1314-19021314-23212023-09-0127314716010.7546/ijba.2023.27.3.000930Adaptive Fed-batch Control of Escherichia coli Fermentation for Protein ProductionVelislava Lyubenova0Anastasiya ZlatkovaMaya IgnatovaDepartment of Mechatronic and Bio/technological Systems, Institute of Robotics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 2, P.O. Box 79 A new adaptive linearizing control algorithm that stabilizes the carbon source concentration in a desired value is proposed. This algorithm is applied to recombinant protein production by Escherichia coli. A model for control of the investigated process is derived. The model identification is made based on experimental data of the batch phase of the process. The operating model includes two sub-models. Each of them describes one of the two physiological states through which the process passes. Switching from one model to another depends on the sign of a key parameter obtained from the acetate measurements. A cascade scheme of software sensors for the estimation of two biomass growth rates included in the structure of the proposed control algorithm is derived. Simulation studies of the developed closed system have been carried out. The results of the impact of an open-loop control system on the same object are compared.http://www.biomed.bas.bg/bioautomation/2023/vol_27.3/files/27.3_03.pdffermentationadaptive controlglucoseacetaterecombinant proteins |
| spellingShingle | Velislava Lyubenova Anastasiya Zlatkova Maya Ignatova Adaptive Fed-batch Control of Escherichia coli Fermentation for Protein Production International Journal Bioautomation fermentation adaptive control glucose acetate recombinant proteins |
| title | Adaptive Fed-batch Control of Escherichia coli Fermentation for Protein Production |
| title_full | Adaptive Fed-batch Control of Escherichia coli Fermentation for Protein Production |
| title_fullStr | Adaptive Fed-batch Control of Escherichia coli Fermentation for Protein Production |
| title_full_unstemmed | Adaptive Fed-batch Control of Escherichia coli Fermentation for Protein Production |
| title_short | Adaptive Fed-batch Control of Escherichia coli Fermentation for Protein Production |
| title_sort | adaptive fed batch control of escherichia coli fermentation for protein production |
| topic | fermentation adaptive control glucose acetate recombinant proteins |
| url | http://www.biomed.bas.bg/bioautomation/2023/vol_27.3/files/27.3_03.pdf |
| work_keys_str_mv | AT velislavalyubenova adaptivefedbatchcontrolofescherichiacolifermentationforproteinproduction AT anastasiyazlatkova adaptivefedbatchcontrolofescherichiacolifermentationforproteinproduction AT mayaignatova adaptivefedbatchcontrolofescherichiacolifermentationforproteinproduction |