Automated measurement of fluorescence signals reveals a significant increase of the graft-derived neurite extension in neonates compared to aged rats
Background: – Neural tissue grafting is an acceptable form of cell therapy for brain injury and diseases. However, methods that can evaluate the graft integration and measure axonal extensions in a 3D environment are limited in scale, inconvenient, and operator intensive. Method: – We stained grafts...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Regenerative Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320422000025 |
| _version_ | 1818353436152823808 |
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| author | Luc Grinand Jun Takahashi |
| author_facet | Luc Grinand Jun Takahashi |
| author_sort | Luc Grinand |
| collection | DOAJ |
| description | Background: – Neural tissue grafting is an acceptable form of cell therapy for brain injury and diseases. However, methods that can evaluate the graft integration and measure axonal extensions in a 3D environment are limited in scale, inconvenient, and operator intensive. Method: – We stained grafts with a fluorescent antibody and then quantified the amount of fluorescence through the entire brain. To achieve this, we created an automated computer program designed to sort out authentic staining from background noise without any user input, enabling the analysis of thousands of images. Results: – Our program could compensate for variations in the background brightness between images in all animals. Using this program, we show that human induced pluripotent stem cell (iPSC)-derived dopaminergic (DA) progenitor cells integrate better into the striatum of neonates than older rats. Conclusion: – Our program can quantify quickly and conveniently the integration of neural grafts in a 3D environment without depending on a blinded human operator. We expect this method to be a useful tool to assess the efficiency of graft-enhancing treatments for neurodegenerative diseases or other neural reconstruction attempts. |
| first_indexed | 2024-12-13T19:09:30Z |
| format | Article |
| id | doaj.art-ddea459cb8a7401da7a893f971935821 |
| institution | Directory Open Access Journal |
| issn | 2352-3204 |
| language | English |
| last_indexed | 2024-12-13T19:09:30Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj.art-ddea459cb8a7401da7a893f9719358212022-12-21T23:34:26ZengElsevierRegenerative Therapy2352-32042022-03-011997106Automated measurement of fluorescence signals reveals a significant increase of the graft-derived neurite extension in neonates compared to aged ratsLuc Grinand0Jun Takahashi1Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University, Kyoto, JapanCorresponding author. Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University, 53 Shogoin-Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan. Fax.: +81 75 366 7071.; Department of Clinical Application, Center for iPS Cell Research and Application, Kyoto University, Kyoto, JapanBackground: – Neural tissue grafting is an acceptable form of cell therapy for brain injury and diseases. However, methods that can evaluate the graft integration and measure axonal extensions in a 3D environment are limited in scale, inconvenient, and operator intensive. Method: – We stained grafts with a fluorescent antibody and then quantified the amount of fluorescence through the entire brain. To achieve this, we created an automated computer program designed to sort out authentic staining from background noise without any user input, enabling the analysis of thousands of images. Results: – Our program could compensate for variations in the background brightness between images in all animals. Using this program, we show that human induced pluripotent stem cell (iPSC)-derived dopaminergic (DA) progenitor cells integrate better into the striatum of neonates than older rats. Conclusion: – Our program can quantify quickly and conveniently the integration of neural grafts in a 3D environment without depending on a blinded human operator. We expect this method to be a useful tool to assess the efficiency of graft-enhancing treatments for neurodegenerative diseases or other neural reconstruction attempts.http://www.sciencedirect.com/science/article/pii/S2352320422000025Neural graftParkinson's diseaseAgingQuantificationAutomation |
| spellingShingle | Luc Grinand Jun Takahashi Automated measurement of fluorescence signals reveals a significant increase of the graft-derived neurite extension in neonates compared to aged rats Regenerative Therapy Neural graft Parkinson's disease Aging Quantification Automation |
| title | Automated measurement of fluorescence signals reveals a significant increase of the graft-derived neurite extension in neonates compared to aged rats |
| title_full | Automated measurement of fluorescence signals reveals a significant increase of the graft-derived neurite extension in neonates compared to aged rats |
| title_fullStr | Automated measurement of fluorescence signals reveals a significant increase of the graft-derived neurite extension in neonates compared to aged rats |
| title_full_unstemmed | Automated measurement of fluorescence signals reveals a significant increase of the graft-derived neurite extension in neonates compared to aged rats |
| title_short | Automated measurement of fluorescence signals reveals a significant increase of the graft-derived neurite extension in neonates compared to aged rats |
| title_sort | automated measurement of fluorescence signals reveals a significant increase of the graft derived neurite extension in neonates compared to aged rats |
| topic | Neural graft Parkinson's disease Aging Quantification Automation |
| url | http://www.sciencedirect.com/science/article/pii/S2352320422000025 |
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