Transluminal Pillars—Their Origin and Role in the Remodelling of the Zebrafish Caudal Vein Plexus
Intussusceptive pillars, regarded as a hallmark of intussusceptive angiogenesis, have been described in developing vasculature of many organs and organisms. The aim of this study was to resolve the question about pillar formation and their further maturation employing zebrafish caudal vein plexus (C...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/23/16703 |
| _version_ | 1797400146414141440 |
|---|---|
| author | Helena Röss Dea Aaldijk Mykhailo Vladymyrov Adolfo Odriozola Valentin Djonov |
| author_facet | Helena Röss Dea Aaldijk Mykhailo Vladymyrov Adolfo Odriozola Valentin Djonov |
| author_sort | Helena Röss |
| collection | DOAJ |
| description | Intussusceptive pillars, regarded as a hallmark of intussusceptive angiogenesis, have been described in developing vasculature of many organs and organisms. The aim of this study was to resolve the question about pillar formation and their further maturation employing zebrafish caudal vein plexus (CVP). The CVP development was monitored by in vivo confocal microscopy in high spatio-temporal resolution using the transgenic zebrafish model <i>Fli1a:eGPF//Gata1:dsRed</i>. We tracked back the formation of pillars (diameter ≤ 4 µm) and intercapillary meshes (diameter > 4 µm) and analysed their morphology and behaviour. Transluminal pillars in the CVP arose via a combination of sprouting, lumen expansion, and/or the creation of intraluminal folds, and those mechanisms were not associated directly with blood flow. The follow-up of pillars indicated that one-third of them disappeared between 28 and 48 h post fertilisation (hpf), and of the remaining ones, only 1/17 changed their cross-section area by >50%. The majority of the bigger meshes (39/62) increased their cross-section area by >50%. Plexus simplification and the establishment of hierarchy were dominated by the dynamics of intercapillary meshes, which formed mainly via sprouting angiogenesis. These meshes were observed to grow, reshape, and merge with each other. Our observations suggested an alternative view on intussusceptive angiogenesis in the CVP. |
| first_indexed | 2024-03-09T01:50:18Z |
| format | Article |
| id | doaj.art-ddeaf6759f5341448a820efd0cfe146f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T01:50:18Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ddeaf6759f5341448a820efd0cfe146f2023-12-08T15:16:50ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124231670310.3390/ijms242316703Transluminal Pillars—Their Origin and Role in the Remodelling of the Zebrafish Caudal Vein PlexusHelena Röss0Dea Aaldijk1Mykhailo Vladymyrov2Adolfo Odriozola3Valentin Djonov4Institute of Anatomy, University of Bern, 3012 Bern, SwitzerlandInstitute of Anatomy, University of Bern, 3012 Bern, SwitzerlandData Science Laboratory, University of Bern, 3012 Bern, SwitzerlandInstitute of Anatomy, University of Bern, 3012 Bern, SwitzerlandInstitute of Anatomy, University of Bern, 3012 Bern, SwitzerlandIntussusceptive pillars, regarded as a hallmark of intussusceptive angiogenesis, have been described in developing vasculature of many organs and organisms. The aim of this study was to resolve the question about pillar formation and their further maturation employing zebrafish caudal vein plexus (CVP). The CVP development was monitored by in vivo confocal microscopy in high spatio-temporal resolution using the transgenic zebrafish model <i>Fli1a:eGPF//Gata1:dsRed</i>. We tracked back the formation of pillars (diameter ≤ 4 µm) and intercapillary meshes (diameter > 4 µm) and analysed their morphology and behaviour. Transluminal pillars in the CVP arose via a combination of sprouting, lumen expansion, and/or the creation of intraluminal folds, and those mechanisms were not associated directly with blood flow. The follow-up of pillars indicated that one-third of them disappeared between 28 and 48 h post fertilisation (hpf), and of the remaining ones, only 1/17 changed their cross-section area by >50%. The majority of the bigger meshes (39/62) increased their cross-section area by >50%. Plexus simplification and the establishment of hierarchy were dominated by the dynamics of intercapillary meshes, which formed mainly via sprouting angiogenesis. These meshes were observed to grow, reshape, and merge with each other. Our observations suggested an alternative view on intussusceptive angiogenesis in the CVP.https://www.mdpi.com/1422-0067/24/23/16703intussusceptive angiogenesiscaudal vein plexustransluminal pillarvessel remodelling |
| spellingShingle | Helena Röss Dea Aaldijk Mykhailo Vladymyrov Adolfo Odriozola Valentin Djonov Transluminal Pillars—Their Origin and Role in the Remodelling of the Zebrafish Caudal Vein Plexus International Journal of Molecular Sciences intussusceptive angiogenesis caudal vein plexus transluminal pillar vessel remodelling |
| title | Transluminal Pillars—Their Origin and Role in the Remodelling of the Zebrafish Caudal Vein Plexus |
| title_full | Transluminal Pillars—Their Origin and Role in the Remodelling of the Zebrafish Caudal Vein Plexus |
| title_fullStr | Transluminal Pillars—Their Origin and Role in the Remodelling of the Zebrafish Caudal Vein Plexus |
| title_full_unstemmed | Transluminal Pillars—Their Origin and Role in the Remodelling of the Zebrafish Caudal Vein Plexus |
| title_short | Transluminal Pillars—Their Origin and Role in the Remodelling of the Zebrafish Caudal Vein Plexus |
| title_sort | transluminal pillars their origin and role in the remodelling of the zebrafish caudal vein plexus |
| topic | intussusceptive angiogenesis caudal vein plexus transluminal pillar vessel remodelling |
| url | https://www.mdpi.com/1422-0067/24/23/16703 |
| work_keys_str_mv | AT helenaross transluminalpillarstheiroriginandroleintheremodellingofthezebrafishcaudalveinplexus AT deaaaldijk transluminalpillarstheiroriginandroleintheremodellingofthezebrafishcaudalveinplexus AT mykhailovladymyrov transluminalpillarstheiroriginandroleintheremodellingofthezebrafishcaudalveinplexus AT adolfoodriozola transluminalpillarstheiroriginandroleintheremodellingofthezebrafishcaudalveinplexus AT valentindjonov transluminalpillarstheiroriginandroleintheremodellingofthezebrafishcaudalveinplexus |