IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS
Abstract. In present study, we investigated the in vitro ability of autologous dendritic cells loaded with tumor lysate antigens to stimulate cytotoxic and secretory activity of effector cells from patients with colorectal cancer. To this purpose, we generated antigen-primed dendritic cells (DS) der...
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/626 |
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| author | V. V. Kurilin Yu. N. Khantakova I. A. Obleukhova Yu. A. Shevchenko E. V. Kulikova V. K. Yakushenko A. V. Sokolov S. V. Sennikov |
| author_facet | V. V. Kurilin Yu. N. Khantakova I. A. Obleukhova Yu. A. Shevchenko E. V. Kulikova V. K. Yakushenko A. V. Sokolov S. V. Sennikov |
| author_sort | V. V. Kurilin |
| collection | DOAJ |
| description | Abstract. In present study, we investigated the in vitro ability of autologous dendritic cells loaded with tumor lysate antigens to stimulate cytotoxic and secretory activity of effector cells from patients with colorectal cancer. To this purpose, we generated antigen-primed dendritic cells (DS) derived from adherent fraction of peripheral blood mononuclear cells (PBMSc) obtained from patients with colorectal cancer. The DCs were then cocultured with non-adherent mononuclear cells, either in presence of IL-12 and IL-18, or without cytokines. The modulation efficiency was assessed as cytotoxic activity of mononuclear cells towards autologous tumor cells, production of IFNγ, IL-2, IL-4 and the number of perforin-, granzyme B-containing cells. We have demonstrated the ability of dendritic cells to stimulate the in vitro antitumor cytotoxic and secretory activity of effector cells in culture. A combination of dendritic cells with IL-12 and IL-18 is shown to enhance their effects in the mononuclear cells culture, as evidenced by increased percentage of dead autologous tumor cells, higher numbers of perforin(+), granzyme B(+) lymphocytes, and increased production of IFNγ, IL-2 increased. |
| first_indexed | 2024-03-08T05:50:10Z |
| format | Article |
| id | doaj.art-ddeb3fcbef404d60beac8ae6568a4a56 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2024-04-24T06:53:44Z |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-ddeb3fcbef404d60beac8ae6568a4a562024-04-22T13:07:38ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-0115323524610.15789/1563-0625-2013-3-235-246623IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTSV. V. Kurilin0Yu. N. Khantakova1I. A. Obleukhova2Yu. A. Shevchenko3E. V. Kulikova4V. K. Yakushenko5A. V. Sokolov6S. V. Sennikov7Research Institute of Clinical Immunology, Siberian Branch, Russian Academy of Medical Sciences, NovosibirskResearch Institute of Clinical Immunology, Siberian Branch, Russian Academy of Medical Sciences, NovosibirskResearch Institute of Clinical Immunology, Siberian Branch, Russian Academy of Medical Sciences, NovosibirskResearch Institute of Clinical Immunology, Siberian Branch, Russian Academy of Medical Sciences, NovosibirskResearch Institute of Clinical Immunology, Siberian Branch, Russian Academy of Medical Sciences, NovosibirskClinical Hospital No. 1, NovosibirskCity Clinical Hospital No. 1, NovosibirskResearch Institute of Clinical Immunology, Siberian Branch, Russian Academy of Medical Sciences, NovosibirskAbstract. In present study, we investigated the in vitro ability of autologous dendritic cells loaded with tumor lysate antigens to stimulate cytotoxic and secretory activity of effector cells from patients with colorectal cancer. To this purpose, we generated antigen-primed dendritic cells (DS) derived from adherent fraction of peripheral blood mononuclear cells (PBMSc) obtained from patients with colorectal cancer. The DCs were then cocultured with non-adherent mononuclear cells, either in presence of IL-12 and IL-18, or without cytokines. The modulation efficiency was assessed as cytotoxic activity of mononuclear cells towards autologous tumor cells, production of IFNγ, IL-2, IL-4 and the number of perforin-, granzyme B-containing cells. We have demonstrated the ability of dendritic cells to stimulate the in vitro antitumor cytotoxic and secretory activity of effector cells in culture. A combination of dendritic cells with IL-12 and IL-18 is shown to enhance their effects in the mononuclear cells culture, as evidenced by increased percentage of dead autologous tumor cells, higher numbers of perforin(+), granzyme B(+) lymphocytes, and increased production of IFNγ, IL-2 increased.https://www.mimmun.ru/mimmun/article/view/626dendritic cellscolorectal cancercytotoxic activity |
| spellingShingle | V. V. Kurilin Yu. N. Khantakova I. A. Obleukhova Yu. A. Shevchenko E. V. Kulikova V. K. Yakushenko A. V. Sokolov S. V. Sennikov IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS Медицинская иммунология dendritic cells colorectal cancer cytotoxic activity |
| title | IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS |
| title_full | IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS |
| title_fullStr | IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS |
| title_full_unstemmed | IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS |
| title_short | IN VITRO STIMULATION OF ANTITUMOR CYTOTOXIC ACTIVITY OF MONONUCLEAR CELLS FROM COLORECTAL CANCER PATIENTS |
| title_sort | in vitro stimulation of antitumor cytotoxic activity of mononuclear cells from colorectal cancer patients |
| topic | dendritic cells colorectal cancer cytotoxic activity |
| url | https://www.mimmun.ru/mimmun/article/view/626 |
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