In vivo low-dose phase-contrast CT for quantification of functional and anatomical alterations in lungs of an experimental allergic airway disease mouse model
IntroductionSynchrotron-based propagation-based imaging (PBI) is ideally suited for lung imaging and has successfully been applied in a variety of in vivo small animal studies. Virtually all these experiments were tailored to achieve extremely high spatial resolution close to the alveolar level whil...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-02-01
|
| Series: | Frontiers in Medicine |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2024.1338846/full |
| _version_ | 1797316818985025536 |
|---|---|
| author | Christian Dullin Christian Dullin Christian Dullin Jonas Albers Jonas Albers Aishwarya Tagat Andrea Lorenzon Lorenzo D'Amico Lorenzo D'Amico Sabina Chiriotti Nicola Sodini Diego Dreossi Frauke Alves Frauke Alves Frauke Alves Anna Bergamaschi Giuliana Tromba |
| author_facet | Christian Dullin Christian Dullin Christian Dullin Jonas Albers Jonas Albers Aishwarya Tagat Andrea Lorenzon Lorenzo D'Amico Lorenzo D'Amico Sabina Chiriotti Nicola Sodini Diego Dreossi Frauke Alves Frauke Alves Frauke Alves Anna Bergamaschi Giuliana Tromba |
| author_sort | Christian Dullin |
| collection | DOAJ |
| description | IntroductionSynchrotron-based propagation-based imaging (PBI) is ideally suited for lung imaging and has successfully been applied in a variety of in vivo small animal studies. Virtually all these experiments were tailored to achieve extremely high spatial resolution close to the alveolar level while delivering high x-ray doses that would not permit longitudinal studies. However, the main rationale for performing lung imaging studies in vivo in small animal models is the ability to follow disease progression or monitor treatment response in the same animal over time. Thus, an in vivo imaging strategy should ideally allow performing longitudinal studies.MethodsHere, we demonstrate our findings of using PBI-based planar and CT imaging with two different detectors—MÖNCH 0.3 direct conversion detector and a complementary metal-oxide-semiconductor (CMOS) detector (Photonics Science)—in an Ovalbumin induced experimental allergic airway disease mouse model in comparison with healthy controls. The mice were imaged free breathing under isoflurane anesthesia.ResultsAt x-ray dose levels below those once used by commercial small animal CT devices at similar spatial resolutions, we were able to resolve structural changes at a pixel size down to 25 μm and demonstrate the reduction in elastic recoil in the asthmatic mice in cinematic planar x-ray imaging with a frame rate of up to 100 fps.DiscussionThus, we believe that our approach will permit longitudinal small animal lung disease studies, closely following the mice over longer time spans. |
| first_indexed | 2024-03-08T03:24:52Z |
| format | Article |
| id | doaj.art-ddeb511fc419463da673bb9af3fec218 |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-03-08T03:24:52Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-ddeb511fc419463da673bb9af3fec2182024-02-12T04:44:23ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2024-02-011110.3389/fmed.2024.13388461338846In vivo low-dose phase-contrast CT for quantification of functional and anatomical alterations in lungs of an experimental allergic airway disease mouse modelChristian Dullin0Christian Dullin1Christian Dullin2Jonas Albers3Jonas Albers4Aishwarya Tagat5Andrea Lorenzon6Lorenzo D'Amico7Lorenzo D'Amico8Sabina Chiriotti9Nicola Sodini10Diego Dreossi11Frauke Alves12Frauke Alves13Frauke Alves14Anna Bergamaschi15Giuliana Tromba16Institute for Diagnostic and Interventional Radiology, University Medical Center Göttingen, Göttingen, GermanyTranslational Molecular Imaging, Max-Plank-Institute for Multidisciplinary Sciences, Göttingen, GermanyDiagnostic and Interventional Radiology, University Hospital Heidelberg, Heidelberg, GermanyInstitute for Diagnostic and Interventional Radiology, University Medical Center Göttingen, Göttingen, GermanyEuropean Molecular Biology Laboratory, Hamburg Unit c/o Deutsches Elektronen-Synchrotron (DESY), Hamburg, GermanyDepartment of Urology, University Hospital of Saarland, Homburg, GermanyInnova S.p.A., Trieste, ItalyElettra-Sincrotrone Trieste S.C.p.A., Trieste, ItalyDepartment of Physics, University of Trieste, Trieste, ItalyPSD Detector Science and Characterization Group, Paul Scherrer Institute, Villingen, SwitzerlandElettra-Sincrotrone Trieste S.C.p.A., Trieste, ItalyElettra-Sincrotrone Trieste S.C.p.A., Trieste, ItalyInstitute for Diagnostic and Interventional Radiology, University Medical Center Göttingen, Göttingen, GermanyTranslational Molecular Imaging, Max-Plank-Institute for Multidisciplinary Sciences, Göttingen, Germany0Department of Haematology and Medical Oncology, University Medical Center Göttingen, Göttingen, GermanyPSD Detector Science and Characterization Group, Paul Scherrer Institute, Villingen, SwitzerlandElettra-Sincrotrone Trieste S.C.p.A., Trieste, ItalyIntroductionSynchrotron-based propagation-based imaging (PBI) is ideally suited for lung imaging and has successfully been applied in a variety of in vivo small animal studies. Virtually all these experiments were tailored to achieve extremely high spatial resolution close to the alveolar level while delivering high x-ray doses that would not permit longitudinal studies. However, the main rationale for performing lung imaging studies in vivo in small animal models is the ability to follow disease progression or monitor treatment response in the same animal over time. Thus, an in vivo imaging strategy should ideally allow performing longitudinal studies.MethodsHere, we demonstrate our findings of using PBI-based planar and CT imaging with two different detectors—MÖNCH 0.3 direct conversion detector and a complementary metal-oxide-semiconductor (CMOS) detector (Photonics Science)—in an Ovalbumin induced experimental allergic airway disease mouse model in comparison with healthy controls. The mice were imaged free breathing under isoflurane anesthesia.ResultsAt x-ray dose levels below those once used by commercial small animal CT devices at similar spatial resolutions, we were able to resolve structural changes at a pixel size down to 25 μm and demonstrate the reduction in elastic recoil in the asthmatic mice in cinematic planar x-ray imaging with a frame rate of up to 100 fps.DiscussionThus, we believe that our approach will permit longitudinal small animal lung disease studies, closely following the mice over longer time spans.https://www.frontiersin.org/articles/10.3389/fmed.2024.1338846/fullphase contrastallergic airway disease modelslung functionlongitudinal experimentsx-ray dose |
| spellingShingle | Christian Dullin Christian Dullin Christian Dullin Jonas Albers Jonas Albers Aishwarya Tagat Andrea Lorenzon Lorenzo D'Amico Lorenzo D'Amico Sabina Chiriotti Nicola Sodini Diego Dreossi Frauke Alves Frauke Alves Frauke Alves Anna Bergamaschi Giuliana Tromba In vivo low-dose phase-contrast CT for quantification of functional and anatomical alterations in lungs of an experimental allergic airway disease mouse model Frontiers in Medicine phase contrast allergic airway disease models lung function longitudinal experiments x-ray dose |
| title | In vivo low-dose phase-contrast CT for quantification of functional and anatomical alterations in lungs of an experimental allergic airway disease mouse model |
| title_full | In vivo low-dose phase-contrast CT for quantification of functional and anatomical alterations in lungs of an experimental allergic airway disease mouse model |
| title_fullStr | In vivo low-dose phase-contrast CT for quantification of functional and anatomical alterations in lungs of an experimental allergic airway disease mouse model |
| title_full_unstemmed | In vivo low-dose phase-contrast CT for quantification of functional and anatomical alterations in lungs of an experimental allergic airway disease mouse model |
| title_short | In vivo low-dose phase-contrast CT for quantification of functional and anatomical alterations in lungs of an experimental allergic airway disease mouse model |
| title_sort | in vivo low dose phase contrast ct for quantification of functional and anatomical alterations in lungs of an experimental allergic airway disease mouse model |
| topic | phase contrast allergic airway disease models lung function longitudinal experiments x-ray dose |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2024.1338846/full |
| work_keys_str_mv | AT christiandullin invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT christiandullin invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT christiandullin invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT jonasalbers invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT jonasalbers invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT aishwaryatagat invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT andrealorenzon invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT lorenzodamico invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT lorenzodamico invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT sabinachiriotti invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT nicolasodini invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT diegodreossi invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT fraukealves invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT fraukealves invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT fraukealves invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT annabergamaschi invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel AT giulianatromba invivolowdosephasecontrastctforquantificationoffunctionalandanatomicalalterationsinlungsofanexperimentalallergicairwaydiseasemousemodel |