Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways.
Human embryonic stem cells have unique value for regenerative medicine, as they are capable of differentiating into a broad variety of cell types. Therefore, defining the signalling pathways that control early cell fate decisions of pluripotent stem cells represents a major task. Moreover, modelling...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-06-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2700259?pdf=render |
| _version_ | 1818445042800394240 |
|---|---|
| author | Ludovic Vallier Thomas Touboul Zhenzhi Chng Minodora Brimpari Nicholas Hannan Enrique Millan Lucy E Smithers Matthew Trotter Peter Rugg-Gunn Anne Weber Roger A Pedersen |
| author_facet | Ludovic Vallier Thomas Touboul Zhenzhi Chng Minodora Brimpari Nicholas Hannan Enrique Millan Lucy E Smithers Matthew Trotter Peter Rugg-Gunn Anne Weber Roger A Pedersen |
| author_sort | Ludovic Vallier |
| collection | DOAJ |
| description | Human embryonic stem cells have unique value for regenerative medicine, as they are capable of differentiating into a broad variety of cell types. Therefore, defining the signalling pathways that control early cell fate decisions of pluripotent stem cells represents a major task. Moreover, modelling the early steps of embryonic development in vitro may provide the best approach to produce cell types with native properties. Here, we analysed the function of key developmental growth factors such as Activin, FGF and BMP in the control of early cell fate decisions of human pluripotent stem cells. This analysis resulted in the development and validation of chemically defined culture conditions for achieving specification of human embryonic stem cells into neuroectoderm, mesendoderm and into extra-embryonic tissues. Importantly, these defined culture conditions are devoid of factors that could obscure analysis of developmental mechanisms or render the resulting tissues incompatible with future clinical applications. Importantly, the growth factor roles defined using these culture conditions similarly drove differentiation of mouse epiblast stem cells derived from post implantation embryos, thereby reinforcing the hypothesis that epiblast stem cells share a common embryonic identity with human pluripotent stem cells. Therefore the defined growth factor conditions described here represent an essential step toward the production of mature cell types from pluripotent stem cells in conditions fully compatible with clinical use ant also provide a general approach for modelling the early steps of mammalian embryonic development. |
| first_indexed | 2024-12-14T19:25:33Z |
| format | Article |
| id | doaj.art-ddf01b0a556047b18a18c13ffed40993 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T19:25:33Z |
| publishDate | 2009-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ddf01b0a556047b18a18c13ffed409932022-12-21T22:50:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-06-0146e608210.1371/journal.pone.0006082Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways.Ludovic VallierThomas TouboulZhenzhi ChngMinodora BrimpariNicholas HannanEnrique MillanLucy E SmithersMatthew TrotterPeter Rugg-GunnAnne WeberRoger A PedersenHuman embryonic stem cells have unique value for regenerative medicine, as they are capable of differentiating into a broad variety of cell types. Therefore, defining the signalling pathways that control early cell fate decisions of pluripotent stem cells represents a major task. Moreover, modelling the early steps of embryonic development in vitro may provide the best approach to produce cell types with native properties. Here, we analysed the function of key developmental growth factors such as Activin, FGF and BMP in the control of early cell fate decisions of human pluripotent stem cells. This analysis resulted in the development and validation of chemically defined culture conditions for achieving specification of human embryonic stem cells into neuroectoderm, mesendoderm and into extra-embryonic tissues. Importantly, these defined culture conditions are devoid of factors that could obscure analysis of developmental mechanisms or render the resulting tissues incompatible with future clinical applications. Importantly, the growth factor roles defined using these culture conditions similarly drove differentiation of mouse epiblast stem cells derived from post implantation embryos, thereby reinforcing the hypothesis that epiblast stem cells share a common embryonic identity with human pluripotent stem cells. Therefore the defined growth factor conditions described here represent an essential step toward the production of mature cell types from pluripotent stem cells in conditions fully compatible with clinical use ant also provide a general approach for modelling the early steps of mammalian embryonic development.http://europepmc.org/articles/PMC2700259?pdf=render |
| spellingShingle | Ludovic Vallier Thomas Touboul Zhenzhi Chng Minodora Brimpari Nicholas Hannan Enrique Millan Lucy E Smithers Matthew Trotter Peter Rugg-Gunn Anne Weber Roger A Pedersen Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways. PLoS ONE |
| title | Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways. |
| title_full | Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways. |
| title_fullStr | Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways. |
| title_full_unstemmed | Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways. |
| title_short | Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways. |
| title_sort | early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways |
| url | http://europepmc.org/articles/PMC2700259?pdf=render |
| work_keys_str_mv | AT ludovicvallier earlycellfatedecisionsofhumanembryonicstemcellsandmouseepiblaststemcellsarecontrolledbythesamesignallingpathways AT thomastouboul earlycellfatedecisionsofhumanembryonicstemcellsandmouseepiblaststemcellsarecontrolledbythesamesignallingpathways AT zhenzhichng earlycellfatedecisionsofhumanembryonicstemcellsandmouseepiblaststemcellsarecontrolledbythesamesignallingpathways AT minodorabrimpari earlycellfatedecisionsofhumanembryonicstemcellsandmouseepiblaststemcellsarecontrolledbythesamesignallingpathways AT nicholashannan earlycellfatedecisionsofhumanembryonicstemcellsandmouseepiblaststemcellsarecontrolledbythesamesignallingpathways AT enriquemillan earlycellfatedecisionsofhumanembryonicstemcellsandmouseepiblaststemcellsarecontrolledbythesamesignallingpathways AT lucyesmithers earlycellfatedecisionsofhumanembryonicstemcellsandmouseepiblaststemcellsarecontrolledbythesamesignallingpathways AT matthewtrotter earlycellfatedecisionsofhumanembryonicstemcellsandmouseepiblaststemcellsarecontrolledbythesamesignallingpathways AT peterrugggunn earlycellfatedecisionsofhumanembryonicstemcellsandmouseepiblaststemcellsarecontrolledbythesamesignallingpathways AT anneweber earlycellfatedecisionsofhumanembryonicstemcellsandmouseepiblaststemcellsarecontrolledbythesamesignallingpathways AT rogerapedersen earlycellfatedecisionsofhumanembryonicstemcellsandmouseepiblaststemcellsarecontrolledbythesamesignallingpathways |