Protective effects of <it>Launaea procumbens</it> on rat testis damage by CCl<sub>4</sub>
<p>Abstract</p> <p>Background</p> <p>Traditionally various human diseases of kidneys, hormonal imbalance and sexual diseases are treated with <it>Launaea procumbens</it> (L). In the present study protective effects of methanolic extract of <it>Launaea...
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| Format: | Article |
| Language: | English |
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BMC
2012-08-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://www.lipidworld.com/content/11/1/103 |
| _version_ | 1818664983877124096 |
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| author | Khan Rahmat |
| author_facet | Khan Rahmat |
| author_sort | Khan Rahmat |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Traditionally various human diseases of kidneys, hormonal imbalance and sexual diseases are treated with <it>Launaea procumbens</it> (L). In the present study protective effects of methanolic extract of <it>Launaea procumbens</it> (LPME) was evaluated against CCl<sub>4</sub>-induced oxidative damages in rat testis.</p> <p>Methods</p> <p>To examine the protective effects of <it>Launaea procumbens</it> on testis against oxidative stress of carbon tetrachloride in male rat, 30 male albino rats were equally divided into 5 groups (6 rats). First group was given standard diet and drinking water. Second group received CCl<sub>4</sub> 3 ml/kg intraperitoneally (30% in olive oil). Third and forth were given orally 100; 200 mg/kg b.w., in 99.8% dimethyl sulphooxide (DMSO), <it>Launaea procumbens</it> methanolic extracts (LPME) after 48 h of CCl<sub>4</sub> treatment twice a week and sixth group received only LPME in DMSO at a dose of 200 mg/kg b.w., for four weeks. Protective effects of <it>Launaea procumbens</it> were observed on sperm concentration, motility and morphology, serum reproductive hormonal level, activity of antioxidant enzymes, lipid peroxidation (TBARS) and DNA damages.</p> <p>Results</p> <p>Results of the present study revealed that treatment of CCl<sub>4</sub> significantly <it>(p < 0.01)</it> reduced sperm concentration and motility comparatively to controls. Level of testosterone, luteinizing hormone and follicle stimulating hormone, were depleted markedly (<it>p <0.01</it>) with treatment of CCl<sub>4</sub>. In addition, CCl<sub>4</sub> induction in rats reduced activities of antioxidant enzymes while increased lipid peroxidation and DNA damages. Co-administration of LPME significantly (<it>p <0.01</it>) improved these alterations in improving of hormonal level, activities of antioxidant enzymes and lipid peroxidation near to control rats.</p> <p>Conclusion</p> <p>From the results it is suggested that <it>Launaea procumbens</it> methanolic extract has the ability to protect testis against oxidative damages, possibly through antioxidant effects of its bioactive compounds.</p> |
| first_indexed | 2024-12-17T05:41:25Z |
| format | Article |
| id | doaj.art-ddf2b128aedb4715b7771b4057e4691d |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-17T05:41:25Z |
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| series | Lipids in Health and Disease |
| spelling | doaj.art-ddf2b128aedb4715b7771b4057e4691d2022-12-21T22:01:26ZengBMCLipids in Health and Disease1476-511X2012-08-0111110310.1186/1476-511X-11-103Protective effects of <it>Launaea procumbens</it> on rat testis damage by CCl<sub>4</sub>Khan Rahmat<p>Abstract</p> <p>Background</p> <p>Traditionally various human diseases of kidneys, hormonal imbalance and sexual diseases are treated with <it>Launaea procumbens</it> (L). In the present study protective effects of methanolic extract of <it>Launaea procumbens</it> (LPME) was evaluated against CCl<sub>4</sub>-induced oxidative damages in rat testis.</p> <p>Methods</p> <p>To examine the protective effects of <it>Launaea procumbens</it> on testis against oxidative stress of carbon tetrachloride in male rat, 30 male albino rats were equally divided into 5 groups (6 rats). First group was given standard diet and drinking water. Second group received CCl<sub>4</sub> 3 ml/kg intraperitoneally (30% in olive oil). Third and forth were given orally 100; 200 mg/kg b.w., in 99.8% dimethyl sulphooxide (DMSO), <it>Launaea procumbens</it> methanolic extracts (LPME) after 48 h of CCl<sub>4</sub> treatment twice a week and sixth group received only LPME in DMSO at a dose of 200 mg/kg b.w., for four weeks. Protective effects of <it>Launaea procumbens</it> were observed on sperm concentration, motility and morphology, serum reproductive hormonal level, activity of antioxidant enzymes, lipid peroxidation (TBARS) and DNA damages.</p> <p>Results</p> <p>Results of the present study revealed that treatment of CCl<sub>4</sub> significantly <it>(p < 0.01)</it> reduced sperm concentration and motility comparatively to controls. Level of testosterone, luteinizing hormone and follicle stimulating hormone, were depleted markedly (<it>p <0.01</it>) with treatment of CCl<sub>4</sub>. In addition, CCl<sub>4</sub> induction in rats reduced activities of antioxidant enzymes while increased lipid peroxidation and DNA damages. Co-administration of LPME significantly (<it>p <0.01</it>) improved these alterations in improving of hormonal level, activities of antioxidant enzymes and lipid peroxidation near to control rats.</p> <p>Conclusion</p> <p>From the results it is suggested that <it>Launaea procumbens</it> methanolic extract has the ability to protect testis against oxidative damages, possibly through antioxidant effects of its bioactive compounds.</p>http://www.lipidworld.com/content/11/1/103<it>Launaea procumbens</it>GSHCCl<sub>4</sub>LipidperoxidationDNA damages |
| spellingShingle | Khan Rahmat Protective effects of <it>Launaea procumbens</it> on rat testis damage by CCl<sub>4</sub> Lipids in Health and Disease <it>Launaea procumbens</it> GSH CCl<sub>4</sub> Lipidperoxidation DNA damages |
| title | Protective effects of <it>Launaea procumbens</it> on rat testis damage by CCl<sub>4</sub> |
| title_full | Protective effects of <it>Launaea procumbens</it> on rat testis damage by CCl<sub>4</sub> |
| title_fullStr | Protective effects of <it>Launaea procumbens</it> on rat testis damage by CCl<sub>4</sub> |
| title_full_unstemmed | Protective effects of <it>Launaea procumbens</it> on rat testis damage by CCl<sub>4</sub> |
| title_short | Protective effects of <it>Launaea procumbens</it> on rat testis damage by CCl<sub>4</sub> |
| title_sort | protective effects of it launaea procumbens it on rat testis damage by ccl sub 4 sub |
| topic | <it>Launaea procumbens</it> GSH CCl<sub>4</sub> Lipidperoxidation DNA damages |
| url | http://www.lipidworld.com/content/11/1/103 |
| work_keys_str_mv | AT khanrahmat protectiveeffectsofitlaunaeaprocumbensitonrattestisdamagebycclsub4sub |