Genetic Requirements for Conjugational Recombination in the Presence of λ Gam Protein in Escherichia coli
The Gam protein of phage is a well-known inhibitor of the enzymatic activities of the RecBCD enzyme, the major enzyme involved in homologous recombination in bacteria Escherichia coli. In this work, we studied (i) the effect of the RecA loading-deficient recB (recBD1080A) mutation on conjugational...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Zagreb Faculty of Food Technology and Biotechnology
2002-01-01
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| Series: | Food Technology and Biotechnology |
| Subjects: | |
| Online Access: | http://hrcak.srce.hr/file/263163 |
| Summary: | The Gam protein of phage is a well-known inhibitor of the enzymatic activities of the RecBCD enzyme, the major enzyme involved in homologous recombination in bacteria Escherichia coli. In this work, we studied (i) the effect of the RecA loading-deficient recB (recBD1080A) mutation on conjugational recombination in the presence of phage Gam protein and (ii) additional genetic requirements for the RecBCD-Gam-mediated conjugational recombination. For this purpose, we introduced Gam+ and Gam- expressing plasmids into wild type cells and different mutants of E. coli (recJ, recBD1080A, recB, recN, recF, recR, recO, recD), and determined the yields of recombinants after Hfr mediated conjugation. The obtained results suggest that RecA loading activity is not inhibited by Gam and that conjugational recombination in the presence of Gam is partially dependent on recJ and recO gene products. |
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| ISSN: | 1330-9862 1334-2606 |