Electrochemical Detection of Waterborne Bacteria Using Bi-Functional Magnetic Nanoparticle Conjugates
Detection of microbial contamination in water is imperative to ensure water quality. We have developed an electrochemical method for the detection of <i>E. coli</i> using bi-functional magnetic nanoparticle (MNP) conjugates. The bi-functional MNP conjugates were prepared by terminal-spec...
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| Formato: | Artigo |
| Idioma: | English |
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MDPI AG
2022-01-01
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| coleção: | Biosensors |
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| Acesso em linha: | https://www.mdpi.com/2079-6374/12/1/36 |
| _version_ | 1827666350255898624 |
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| author | Dharanivasan Gunasekaran Yoram Gerchman Sefi Vernick |
| author_facet | Dharanivasan Gunasekaran Yoram Gerchman Sefi Vernick |
| author_sort | Dharanivasan Gunasekaran |
| collection | DOAJ |
| description | Detection of microbial contamination in water is imperative to ensure water quality. We have developed an electrochemical method for the detection of <i>E. coli</i> using bi-functional magnetic nanoparticle (MNP) conjugates. The bi-functional MNP conjugates were prepared by terminal-specific conjugation of anti-<i>E. coli</i> IgG antibody and the electroactive marker ferrocene. The bi-functional MNP conjugate possesses both <i>E. coli</i>-specific binding and electroactive properties, which were studied in detail. The conjugation efficiency of ferrocene and IgG antibodies with amine-functionalized MNPs was investigated. Square-wave voltammetry enabled the detection of <i>E. coli</i> concentrations ranging from 10<sup>1</sup>–10<sup>7</sup> cells/mL in a dose-dependent manner, as ferrocene-specific current signals were inversely dependent on <i>E. coli</i> concentrations, completely suppressed at concentrations higher than 10<sup>7</sup> cells/mL. The developed electrochemical method is highly sensitive (10 cells/mL) and, coupled to magnetic separation, provides specific signals within 1h. Overall, the bi-functional conjugates serve as ideal candidates for electrochemical detection of waterborne bacteria. This approach can be applied for the detection of other bacteria and viruses. |
| first_indexed | 2024-03-10T01:49:31Z |
| format | Article |
| id | doaj.art-ddf606a47ff24b4c86ac20f8caffec2f |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T01:49:31Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-ddf606a47ff24b4c86ac20f8caffec2f2023-11-23T13:08:20ZengMDPI AGBiosensors2079-63742022-01-011213610.3390/bios12010036Electrochemical Detection of Waterborne Bacteria Using Bi-Functional Magnetic Nanoparticle ConjugatesDharanivasan Gunasekaran0Yoram Gerchman1Sefi Vernick2Department of Sensing, Information and Mechanization Engineering, Institute of Agricultural Engineering, Volcani Institute (ARO), Rishon leZion 5025001, IsraelThe Faculty of Natural Sciences, Oranim Academic College of Education, The University of Haifa, Tivon 3600600, IsraelDepartment of Sensing, Information and Mechanization Engineering, Institute of Agricultural Engineering, Volcani Institute (ARO), Rishon leZion 5025001, IsraelDetection of microbial contamination in water is imperative to ensure water quality. We have developed an electrochemical method for the detection of <i>E. coli</i> using bi-functional magnetic nanoparticle (MNP) conjugates. The bi-functional MNP conjugates were prepared by terminal-specific conjugation of anti-<i>E. coli</i> IgG antibody and the electroactive marker ferrocene. The bi-functional MNP conjugate possesses both <i>E. coli</i>-specific binding and electroactive properties, which were studied in detail. The conjugation efficiency of ferrocene and IgG antibodies with amine-functionalized MNPs was investigated. Square-wave voltammetry enabled the detection of <i>E. coli</i> concentrations ranging from 10<sup>1</sup>–10<sup>7</sup> cells/mL in a dose-dependent manner, as ferrocene-specific current signals were inversely dependent on <i>E. coli</i> concentrations, completely suppressed at concentrations higher than 10<sup>7</sup> cells/mL. The developed electrochemical method is highly sensitive (10 cells/mL) and, coupled to magnetic separation, provides specific signals within 1h. Overall, the bi-functional conjugates serve as ideal candidates for electrochemical detection of waterborne bacteria. This approach can be applied for the detection of other bacteria and viruses.https://www.mdpi.com/2079-6374/12/1/36anti-<i>E. coli</i> antibodybi-functional MNP conjugateferrocene carboxylic acidelectro-chemical detectionwaterborne bacterial contamination |
| spellingShingle | Dharanivasan Gunasekaran Yoram Gerchman Sefi Vernick Electrochemical Detection of Waterborne Bacteria Using Bi-Functional Magnetic Nanoparticle Conjugates Biosensors anti-<i>E. coli</i> antibody bi-functional MNP conjugate ferrocene carboxylic acid electro-chemical detection waterborne bacterial contamination |
| title | Electrochemical Detection of Waterborne Bacteria Using Bi-Functional Magnetic Nanoparticle Conjugates |
| title_full | Electrochemical Detection of Waterborne Bacteria Using Bi-Functional Magnetic Nanoparticle Conjugates |
| title_fullStr | Electrochemical Detection of Waterborne Bacteria Using Bi-Functional Magnetic Nanoparticle Conjugates |
| title_full_unstemmed | Electrochemical Detection of Waterborne Bacteria Using Bi-Functional Magnetic Nanoparticle Conjugates |
| title_short | Electrochemical Detection of Waterborne Bacteria Using Bi-Functional Magnetic Nanoparticle Conjugates |
| title_sort | electrochemical detection of waterborne bacteria using bi functional magnetic nanoparticle conjugates |
| topic | anti-<i>E. coli</i> antibody bi-functional MNP conjugate ferrocene carboxylic acid electro-chemical detection waterborne bacterial contamination |
| url | https://www.mdpi.com/2079-6374/12/1/36 |
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