The impact of respiration and oxidative stress response on recombinant α-amylase production by Saccharomyces cerevisiae
Studying protein production is important for fundamental research on cell biology and applied research for biotechnology. Yeast Saccharomyces cerevisiae is an attractive workhorse for production of recombinant proteins as it does not secrete many endogenous proteins and it is therefore easy to purif...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-12-01
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| Series: | Metabolic Engineering Communications |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214030116300219 |
| _version_ | 1811215392547274752 |
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| author | José L. Martínez Eugenio Meza Dina Petranovic Jens Nielsen |
| author_facet | José L. Martínez Eugenio Meza Dina Petranovic Jens Nielsen |
| author_sort | José L. Martínez |
| collection | DOAJ |
| description | Studying protein production is important for fundamental research on cell biology and applied research for biotechnology. Yeast Saccharomyces cerevisiae is an attractive workhorse for production of recombinant proteins as it does not secrete many endogenous proteins and it is therefore easy to purify a secreted product. However, recombinant production at high rates represents a significant metabolic burden for the yeast cells, which results in oxidative stress and ultimately affects the protein production capacity. Here we describe a method to reduce the overall oxidative stress by overexpressing the endogenous HAP1 gene in a S. cerevisiae strain overproducing recombinant α-amylase. We demonstrate how Hap1p can activate a set of oxidative stress response genes and meanwhile contribute to increase the metabolic rate of the yeast strains, therefore mitigating the negative effect of the ROS accumulation associated to protein folding and hence increasing the production capacity during batch fermentations. Keywords: Yeast, Oxidative stress response, Protein production, Hap1, Amylase |
| first_indexed | 2024-04-12T06:21:54Z |
| format | Article |
| id | doaj.art-ddf7585d319143d1badbe14bd06482fe |
| institution | Directory Open Access Journal |
| issn | 2214-0301 |
| language | English |
| last_indexed | 2024-04-12T06:21:54Z |
| publishDate | 2016-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Metabolic Engineering Communications |
| spelling | doaj.art-ddf7585d319143d1badbe14bd06482fe2022-12-22T03:44:18ZengElsevierMetabolic Engineering Communications2214-03012016-12-013205210The impact of respiration and oxidative stress response on recombinant α-amylase production by Saccharomyces cerevisiaeJosé L. Martínez0Eugenio Meza1Dina Petranovic2Jens Nielsen3Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, SE41296 Gothenburg, Sweden; Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 41296 Göteborg, SwedenDepartment of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 41296 Göteborg, SwedenNovo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, SE41296 Gothenburg, Sweden; Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 41296 Göteborg, SwedenNovo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology, SE41296 Gothenburg, Sweden; Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 41296 Göteborg, Sweden; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, DK2970 Hørsholm, Denmark; Corresponding author at: Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 41296 Göteborg, Sweden.Studying protein production is important for fundamental research on cell biology and applied research for biotechnology. Yeast Saccharomyces cerevisiae is an attractive workhorse for production of recombinant proteins as it does not secrete many endogenous proteins and it is therefore easy to purify a secreted product. However, recombinant production at high rates represents a significant metabolic burden for the yeast cells, which results in oxidative stress and ultimately affects the protein production capacity. Here we describe a method to reduce the overall oxidative stress by overexpressing the endogenous HAP1 gene in a S. cerevisiae strain overproducing recombinant α-amylase. We demonstrate how Hap1p can activate a set of oxidative stress response genes and meanwhile contribute to increase the metabolic rate of the yeast strains, therefore mitigating the negative effect of the ROS accumulation associated to protein folding and hence increasing the production capacity during batch fermentations. Keywords: Yeast, Oxidative stress response, Protein production, Hap1, Amylasehttp://www.sciencedirect.com/science/article/pii/S2214030116300219 |
| spellingShingle | José L. Martínez Eugenio Meza Dina Petranovic Jens Nielsen The impact of respiration and oxidative stress response on recombinant α-amylase production by Saccharomyces cerevisiae Metabolic Engineering Communications |
| title | The impact of respiration and oxidative stress response on recombinant α-amylase production by Saccharomyces cerevisiae |
| title_full | The impact of respiration and oxidative stress response on recombinant α-amylase production by Saccharomyces cerevisiae |
| title_fullStr | The impact of respiration and oxidative stress response on recombinant α-amylase production by Saccharomyces cerevisiae |
| title_full_unstemmed | The impact of respiration and oxidative stress response on recombinant α-amylase production by Saccharomyces cerevisiae |
| title_short | The impact of respiration and oxidative stress response on recombinant α-amylase production by Saccharomyces cerevisiae |
| title_sort | impact of respiration and oxidative stress response on recombinant α amylase production by saccharomyces cerevisiae |
| url | http://www.sciencedirect.com/science/article/pii/S2214030116300219 |
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