Vaccination with Formulation of Nanoparticles Loaded with <i>Leishmania amazonensis</i> Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster
Visceral leishmaniasis (VL) is a fatal disease caused by the protozoa <i>Leishmania infantum</i> for which dogs are the main reservoirs. A vaccine against canine visceral leishmaniasis (CVL) could be an important tool in the control of human and CVL by reducing the infection pressure of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/11/1/111 |
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| author | Marco Antonio Cabrera González Ana Alice Maia Gonçalves Jennifer Ottino Jaqueline Costa Leite Lucilene Aparecida Resende Otoni Alves Melo-Júnior Patrícia Silveira Mariana Santos Cardoso Ricardo Toshio Fujiwara Lilian Lacerda Bueno Renato Lima Santos Tatiane Furtado de Carvalho Giani Martins Garcia Paulo Ricardo de Oliveira Paes Alexsandro Sobreira Galdino Miguel Angel Chávez-Fumagalli Marília Martins Melo Denise Silveira-Lemos Olindo Assis Martins-Filho Walderez Ornelas Dutra Vanessa Carla Furtado Mosqueira Rodolfo Cordeiro Giunchetti |
| author_facet | Marco Antonio Cabrera González Ana Alice Maia Gonçalves Jennifer Ottino Jaqueline Costa Leite Lucilene Aparecida Resende Otoni Alves Melo-Júnior Patrícia Silveira Mariana Santos Cardoso Ricardo Toshio Fujiwara Lilian Lacerda Bueno Renato Lima Santos Tatiane Furtado de Carvalho Giani Martins Garcia Paulo Ricardo de Oliveira Paes Alexsandro Sobreira Galdino Miguel Angel Chávez-Fumagalli Marília Martins Melo Denise Silveira-Lemos Olindo Assis Martins-Filho Walderez Ornelas Dutra Vanessa Carla Furtado Mosqueira Rodolfo Cordeiro Giunchetti |
| author_sort | Marco Antonio Cabrera González |
| collection | DOAJ |
| description | Visceral leishmaniasis (VL) is a fatal disease caused by the protozoa <i>Leishmania infantum</i> for which dogs are the main reservoirs. A vaccine against canine visceral leishmaniasis (CVL) could be an important tool in the control of human and CVL by reducing the infection pressure of <i>L. infantum</i>. Despite the CVL vaccine available on the market, the Brazilian Ministry of Health did not implement the use of it in their control programs. In this sense, there is an urgent need to develop more efficient vaccines. In this study, the association between two polymeric nanoformulations, (poly (D, L-lactic) acid (PLA) polymer) loading <i>Leishmania amazonensis</i> antigens, was evaluated as a potential immunobiological agent against VL using golden hamsters as an experimental model. The results indicated that no significant adverse reactions were observed in animals vaccinated with LAPSmP. LAPSmP presented similar levels of total anti-<i>Leishmania</i> IgG as compared to LAPSmG. The LAPSmP and LAPSmG groups showed an intense reduction in liver and spleen parasitic load by qPCR. The LAPSmP and LAPSmG vaccines showed exceptional results, indicating that they may be promising candidates as a VL vaccine. |
| first_indexed | 2024-03-09T11:04:36Z |
| format | Article |
| id | doaj.art-ddf88c251c53434abf048b15b03ee2b9 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T11:04:36Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-ddf88c251c53434abf048b15b03ee2b92023-12-01T01:02:40ZengMDPI AGVaccines2076-393X2023-01-0111111110.3390/vaccines11010111Vaccination with Formulation of Nanoparticles Loaded with <i>Leishmania amazonensis</i> Antigens Confers Protection against Experimental Visceral Leishmaniasis in HamsterMarco Antonio Cabrera González0Ana Alice Maia Gonçalves1Jennifer Ottino2Jaqueline Costa Leite3Lucilene Aparecida Resende4Otoni Alves Melo-Júnior5Patrícia Silveira6Mariana Santos Cardoso7Ricardo Toshio Fujiwara8Lilian Lacerda Bueno9Renato Lima Santos10Tatiane Furtado de Carvalho11Giani Martins Garcia12Paulo Ricardo de Oliveira Paes13Alexsandro Sobreira Galdino14Miguel Angel Chávez-Fumagalli15Marília Martins Melo16Denise Silveira-Lemos17Olindo Assis Martins-Filho18Walderez Ornelas Dutra19Vanessa Carla Furtado Mosqueira20Rodolfo Cordeiro Giunchetti21Departamento de Morfologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilDepartamento de Morfologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilDepartamento de Parasitologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilDepartamento de Morfologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilDepartamento de Morfologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilDepartamento de Morfologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilDepartamento de Morfologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilDepartamento de Parasitologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilDepartamento de Parasitologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilDepartamento de Parasitologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilEscola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilEscola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilLaboratório de Desenvolvimento Galênico e Nanotecnologia, Escola de Farmácia, Universidade Federal de Ouro Preto (UFOP), Ouro Preto 35400-000, MG, BrazilEscola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilLaboratório de Biotecnologia de Microrganismos, Universidade Federal de São João Del-Rei (UFSJ), Campus Centro Oeste, Divinópolis 35501-296, MG, BrazilComputational Biology and Chemistry Research Group, Vicerrectorado de Investigación, Universidad Católica de Santa María, Urb. San José S/N, Arequipa 04000, PeruEscola de Veterinária, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilDepartamento de Morfologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilFIOCRUZ-Minas Gerais, Laboratório de Biomarcadores de Diagnóstico e Monitoração, Instituto René Rachou, Belo Horizonte 30190-002, MG, BrazilDepartamento de Morfologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilLaboratório de Desenvolvimento Galênico e Nanotecnologia, Escola de Farmácia, Universidade Federal de Ouro Preto (UFOP), Ouro Preto 35400-000, MG, BrazilDepartamento de Morfologia, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 31270-901, MG, BrazilVisceral leishmaniasis (VL) is a fatal disease caused by the protozoa <i>Leishmania infantum</i> for which dogs are the main reservoirs. A vaccine against canine visceral leishmaniasis (CVL) could be an important tool in the control of human and CVL by reducing the infection pressure of <i>L. infantum</i>. Despite the CVL vaccine available on the market, the Brazilian Ministry of Health did not implement the use of it in their control programs. In this sense, there is an urgent need to develop more efficient vaccines. In this study, the association between two polymeric nanoformulations, (poly (D, L-lactic) acid (PLA) polymer) loading <i>Leishmania amazonensis</i> antigens, was evaluated as a potential immunobiological agent against VL using golden hamsters as an experimental model. The results indicated that no significant adverse reactions were observed in animals vaccinated with LAPSmP. LAPSmP presented similar levels of total anti-<i>Leishmania</i> IgG as compared to LAPSmG. The LAPSmP and LAPSmG groups showed an intense reduction in liver and spleen parasitic load by qPCR. The LAPSmP and LAPSmG vaccines showed exceptional results, indicating that they may be promising candidates as a VL vaccine.https://www.mdpi.com/2076-393X/11/1/111visceral leishmaniasisnanotechnologyvaccine |
| spellingShingle | Marco Antonio Cabrera González Ana Alice Maia Gonçalves Jennifer Ottino Jaqueline Costa Leite Lucilene Aparecida Resende Otoni Alves Melo-Júnior Patrícia Silveira Mariana Santos Cardoso Ricardo Toshio Fujiwara Lilian Lacerda Bueno Renato Lima Santos Tatiane Furtado de Carvalho Giani Martins Garcia Paulo Ricardo de Oliveira Paes Alexsandro Sobreira Galdino Miguel Angel Chávez-Fumagalli Marília Martins Melo Denise Silveira-Lemos Olindo Assis Martins-Filho Walderez Ornelas Dutra Vanessa Carla Furtado Mosqueira Rodolfo Cordeiro Giunchetti Vaccination with Formulation of Nanoparticles Loaded with <i>Leishmania amazonensis</i> Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster Vaccines visceral leishmaniasis nanotechnology vaccine |
| title | Vaccination with Formulation of Nanoparticles Loaded with <i>Leishmania amazonensis</i> Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster |
| title_full | Vaccination with Formulation of Nanoparticles Loaded with <i>Leishmania amazonensis</i> Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster |
| title_fullStr | Vaccination with Formulation of Nanoparticles Loaded with <i>Leishmania amazonensis</i> Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster |
| title_full_unstemmed | Vaccination with Formulation of Nanoparticles Loaded with <i>Leishmania amazonensis</i> Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster |
| title_short | Vaccination with Formulation of Nanoparticles Loaded with <i>Leishmania amazonensis</i> Antigens Confers Protection against Experimental Visceral Leishmaniasis in Hamster |
| title_sort | vaccination with formulation of nanoparticles loaded with i leishmania amazonensis i antigens confers protection against experimental visceral leishmaniasis in hamster |
| topic | visceral leishmaniasis nanotechnology vaccine |
| url | https://www.mdpi.com/2076-393X/11/1/111 |
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