No-ozone cold plasma can kill oral pathogenic microbes in H2O2-dependent and independent manner
Abstract To apply the sterilisation effect of low-temperature plasma to the oral cavity, the issue of ozone from plasma must be addressed. In this study, a new technology for generating cold plasma with almost no ozone is developed and is named Nozone (no-ozone) Cold Plasma (NCP) technology. The ant...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-11665-z |
| _version_ | 1811253213907648512 |
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| author | Nam-Sook Park Se-Eun Yun Hyun-Young Lee Hae June Lee Jeong-Hae Choi Gyoo-Cheon Kim |
| author_facet | Nam-Sook Park Se-Eun Yun Hyun-Young Lee Hae June Lee Jeong-Hae Choi Gyoo-Cheon Kim |
| author_sort | Nam-Sook Park |
| collection | DOAJ |
| description | Abstract To apply the sterilisation effect of low-temperature plasma to the oral cavity, the issue of ozone from plasma must be addressed. In this study, a new technology for generating cold plasma with almost no ozone is developed and is named Nozone (no-ozone) Cold Plasma (NCP) technology. The antimicrobial efficacy of the NCP against four oral pathogens is tested, and its specific mechanism is elucidated. The treatment of NCP on oral pathogenic microbes on a solid medium generated a growth inhibition zone. When NCP is applied to oral pathogens in a liquid medium, the growth of microbes decreased by more than 105 colony forming units, and the bactericidal effect of NCP remained after the installation of dental tips. The bactericidal effect of NCP in the liquid medium is due to the increase in hydrogen peroxide levels in the medium. However, the bactericidal effect of NCP in the solid medium depends on the charged elements of the NCP. Furthermore, the surface bactericidal efficiency of the dental-tip-installed NCP is proportional to the pore size of the tips and inversely proportional to the length of the tips. Overall, we expect this NCP device to be widely used in dentistry in the near future. |
| first_indexed | 2024-04-12T16:46:32Z |
| format | Article |
| id | doaj.art-ddf8fb954fb049ba8547d5c0b066b79b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T16:46:32Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ddf8fb954fb049ba8547d5c0b066b79b2022-12-22T03:24:33ZengNature PortfolioScientific Reports2045-23222022-05-0112111510.1038/s41598-022-11665-zNo-ozone cold plasma can kill oral pathogenic microbes in H2O2-dependent and independent mannerNam-Sook Park0Se-Eun Yun1Hyun-Young Lee2Hae June Lee3Jeong-Hae Choi4Gyoo-Cheon Kim5Department of Research and Development, FEAGLE CorporationsDepartment of Oral Anatomy and Cell Biology, School of Dentistry, Pusan National UniversityDepartment of Research and Development, FEAGLE CorporationsDepartment of Electrical Engineering, Pusan National UniversityDepartment of Research and Development, FEAGLE CorporationsDepartment of Research and Development, FEAGLE CorporationsAbstract To apply the sterilisation effect of low-temperature plasma to the oral cavity, the issue of ozone from plasma must be addressed. In this study, a new technology for generating cold plasma with almost no ozone is developed and is named Nozone (no-ozone) Cold Plasma (NCP) technology. The antimicrobial efficacy of the NCP against four oral pathogens is tested, and its specific mechanism is elucidated. The treatment of NCP on oral pathogenic microbes on a solid medium generated a growth inhibition zone. When NCP is applied to oral pathogens in a liquid medium, the growth of microbes decreased by more than 105 colony forming units, and the bactericidal effect of NCP remained after the installation of dental tips. The bactericidal effect of NCP in the liquid medium is due to the increase in hydrogen peroxide levels in the medium. However, the bactericidal effect of NCP in the solid medium depends on the charged elements of the NCP. Furthermore, the surface bactericidal efficiency of the dental-tip-installed NCP is proportional to the pore size of the tips and inversely proportional to the length of the tips. Overall, we expect this NCP device to be widely used in dentistry in the near future.https://doi.org/10.1038/s41598-022-11665-z |
| spellingShingle | Nam-Sook Park Se-Eun Yun Hyun-Young Lee Hae June Lee Jeong-Hae Choi Gyoo-Cheon Kim No-ozone cold plasma can kill oral pathogenic microbes in H2O2-dependent and independent manner Scientific Reports |
| title | No-ozone cold plasma can kill oral pathogenic microbes in H2O2-dependent and independent manner |
| title_full | No-ozone cold plasma can kill oral pathogenic microbes in H2O2-dependent and independent manner |
| title_fullStr | No-ozone cold plasma can kill oral pathogenic microbes in H2O2-dependent and independent manner |
| title_full_unstemmed | No-ozone cold plasma can kill oral pathogenic microbes in H2O2-dependent and independent manner |
| title_short | No-ozone cold plasma can kill oral pathogenic microbes in H2O2-dependent and independent manner |
| title_sort | no ozone cold plasma can kill oral pathogenic microbes in h2o2 dependent and independent manner |
| url | https://doi.org/10.1038/s41598-022-11665-z |
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