Dynamic changes in transcripts during regeneration of the secondary vascular system in <it>Populus tomentosa </it>Carr. revealed by cDNA microarrays
<p>Abstract</p> <p>Background</p> <p>Wood is the end product of secondary vascular system development, which begins from the cambium. The wood formation process includes four major stages: cell expansion, secondary wall biosynthesis, lignification, and programmed cell d...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-05-01
|
| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/10/215 |
| _version_ | 1818156542667522048 |
|---|---|
| author | Zhang Shougong Zhao Shutang Qi Xiaoli Wang Minjie Lu Meng-Zhu |
| author_facet | Zhang Shougong Zhao Shutang Qi Xiaoli Wang Minjie Lu Meng-Zhu |
| author_sort | Zhang Shougong |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Wood is the end product of secondary vascular system development, which begins from the cambium. The wood formation process includes four major stages: cell expansion, secondary wall biosynthesis, lignification, and programmed cell death. Transcriptional profiling is a rapid way to screen for genes involved in these stages and their transitions, providing the basis for understanding the molecular mechanisms that control this process.</p> <p>Results</p> <p>In this study, cDNA microarrays were prepared from a subtracted cDNA library (cambium zone <it>versus </it>leaf) of Chinese white poplar (<it>Populus tomentosa </it>Carr.) and employed to analyze the transcriptional profiles during the regeneration of the secondary vascular system, a platform established in our previous study. Two hundred and seven genes showed transcript-level differences at the different regeneration stages. Dramatic transcriptional changes were observed at cambium initiation, cambium formation and differentiation, and xylem development, suggesting that these up- or downregulated genes play important roles in these stage transitions. Transcription factors such as AUX/IAA and PINHEAD, which were previously shown to be involved in meristem and vascular tissue differentiation, were strongly transcribed at the stages when cambial cells were initiated and underwent differentiation, whereas genes encoding MYB proteins and several small heat shock proteins were strongly transcribed at the stage when xylem development begins.</p> <p>Conclusion</p> <p>Employing this method, we observed dynamic changes in gene transcript levels at the key stages, including cambium initiation, cambium formation and differentiation, and xylem development, suggesting that these up- or downregulated genes are strongly involved in these stage transitions. Further studies of these genes could help elucidate their roles in wood formation.</p> |
| first_indexed | 2024-12-11T14:59:57Z |
| format | Article |
| id | doaj.art-ddfc08db2ad848b584311b8265890cc6 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-11T14:59:57Z |
| publishDate | 2009-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-ddfc08db2ad848b584311b8265890cc62022-12-22T01:01:11ZengBMCBMC Genomics1471-21642009-05-0110121510.1186/1471-2164-10-215Dynamic changes in transcripts during regeneration of the secondary vascular system in <it>Populus tomentosa </it>Carr. revealed by cDNA microarraysZhang ShougongZhao ShutangQi XiaoliWang MinjieLu Meng-Zhu<p>Abstract</p> <p>Background</p> <p>Wood is the end product of secondary vascular system development, which begins from the cambium. The wood formation process includes four major stages: cell expansion, secondary wall biosynthesis, lignification, and programmed cell death. Transcriptional profiling is a rapid way to screen for genes involved in these stages and their transitions, providing the basis for understanding the molecular mechanisms that control this process.</p> <p>Results</p> <p>In this study, cDNA microarrays were prepared from a subtracted cDNA library (cambium zone <it>versus </it>leaf) of Chinese white poplar (<it>Populus tomentosa </it>Carr.) and employed to analyze the transcriptional profiles during the regeneration of the secondary vascular system, a platform established in our previous study. Two hundred and seven genes showed transcript-level differences at the different regeneration stages. Dramatic transcriptional changes were observed at cambium initiation, cambium formation and differentiation, and xylem development, suggesting that these up- or downregulated genes play important roles in these stage transitions. Transcription factors such as AUX/IAA and PINHEAD, which were previously shown to be involved in meristem and vascular tissue differentiation, were strongly transcribed at the stages when cambial cells were initiated and underwent differentiation, whereas genes encoding MYB proteins and several small heat shock proteins were strongly transcribed at the stage when xylem development begins.</p> <p>Conclusion</p> <p>Employing this method, we observed dynamic changes in gene transcript levels at the key stages, including cambium initiation, cambium formation and differentiation, and xylem development, suggesting that these up- or downregulated genes are strongly involved in these stage transitions. Further studies of these genes could help elucidate their roles in wood formation.</p>http://www.biomedcentral.com/1471-2164/10/215 |
| spellingShingle | Zhang Shougong Zhao Shutang Qi Xiaoli Wang Minjie Lu Meng-Zhu Dynamic changes in transcripts during regeneration of the secondary vascular system in <it>Populus tomentosa </it>Carr. revealed by cDNA microarrays BMC Genomics |
| title | Dynamic changes in transcripts during regeneration of the secondary vascular system in <it>Populus tomentosa </it>Carr. revealed by cDNA microarrays |
| title_full | Dynamic changes in transcripts during regeneration of the secondary vascular system in <it>Populus tomentosa </it>Carr. revealed by cDNA microarrays |
| title_fullStr | Dynamic changes in transcripts during regeneration of the secondary vascular system in <it>Populus tomentosa </it>Carr. revealed by cDNA microarrays |
| title_full_unstemmed | Dynamic changes in transcripts during regeneration of the secondary vascular system in <it>Populus tomentosa </it>Carr. revealed by cDNA microarrays |
| title_short | Dynamic changes in transcripts during regeneration of the secondary vascular system in <it>Populus tomentosa </it>Carr. revealed by cDNA microarrays |
| title_sort | dynamic changes in transcripts during regeneration of the secondary vascular system in it populus tomentosa it carr revealed by cdna microarrays |
| url | http://www.biomedcentral.com/1471-2164/10/215 |
| work_keys_str_mv | AT zhangshougong dynamicchangesintranscriptsduringregenerationofthesecondaryvascularsysteminitpopulustomentosaitcarrrevealedbycdnamicroarrays AT zhaoshutang dynamicchangesintranscriptsduringregenerationofthesecondaryvascularsysteminitpopulustomentosaitcarrrevealedbycdnamicroarrays AT qixiaoli dynamicchangesintranscriptsduringregenerationofthesecondaryvascularsysteminitpopulustomentosaitcarrrevealedbycdnamicroarrays AT wangminjie dynamicchangesintranscriptsduringregenerationofthesecondaryvascularsysteminitpopulustomentosaitcarrrevealedbycdnamicroarrays AT lumengzhu dynamicchangesintranscriptsduringregenerationofthesecondaryvascularsysteminitpopulustomentosaitcarrrevealedbycdnamicroarrays |