Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils

The purpose of this research was to isolate local bacterial have the effectiveness of de-emulsification in liquid emulsion. Twenty local isolates of actinomycetes were isolated from soil contaminated with oil waste using mineral liquid medium at initial pH of 7 in the presence of oil waste (1%) as a...

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Main Authors: Salah H. Kalaf, Eman H. Gata, Amil A. Halob, Saad H. Khudhair
Format: Article
Language:English
Published: Unviversity of Technology- Iraq 2014-06-01
Series:Engineering and Technology Journal
Subjects:
Online Access:https://etj.uotechnology.edu.iq/article_105287_73e9d20106d18e7a5a7b2cf424dfb70f.pdf
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author Salah H. Kalaf
Eman H. Gata
Amil A. Halob
Saad H. Khudhair
author_facet Salah H. Kalaf
Eman H. Gata
Amil A. Halob
Saad H. Khudhair
author_sort Salah H. Kalaf
collection DOAJ
description The purpose of this research was to isolate local bacterial have the effectiveness of de-emulsification in liquid emulsion. Twenty local isolates of actinomycetes were isolated from soil contaminated with oil waste using mineral liquid medium at initial pH of 7 in the presence of oil waste (1%) as a source of carbon and incubated at 40 °C for 5 days . The primary screening of isolates was tested to de-emulsifying ability.Results indicate that the isolates ST3 , ST8 and ST15 were selected for theirhigh ability to de-emulsification. Secondary screening of selected isolates was estimated to deemulsification using the spore suspension . Results indicate that the ST8 isolate showed the highest de-emulsification activity, subsequently was identified and designated as Nocardia sp.. Studied the effects of both the spores number and period of exposure on the effectiveness of de-emulsification. The results showed that the 0.5 ml of spore suspension (107 spore/ml) gave the best effective way to de-emulsifying, while 30 min. the best period appropriate to de-emulsifying.
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spelling doaj.art-ddfd997d043e4d93bc94502cef205df32024-02-04T17:30:57ZengUnviversity of Technology- IraqEngineering and Technology Journal1681-69002412-07582014-06-01326B808510.30684/etj.32.6B.20105287Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local SoilsSalah H. KalafEman H. GataAmil A. HalobSaad H. KhudhairThe purpose of this research was to isolate local bacterial have the effectiveness of de-emulsification in liquid emulsion. Twenty local isolates of actinomycetes were isolated from soil contaminated with oil waste using mineral liquid medium at initial pH of 7 in the presence of oil waste (1%) as a source of carbon and incubated at 40 °C for 5 days . The primary screening of isolates was tested to de-emulsifying ability.Results indicate that the isolates ST3 , ST8 and ST15 were selected for theirhigh ability to de-emulsification. Secondary screening of selected isolates was estimated to deemulsification using the spore suspension . Results indicate that the ST8 isolate showed the highest de-emulsification activity, subsequently was identified and designated as Nocardia sp.. Studied the effects of both the spores number and period of exposure on the effectiveness of de-emulsification. The results showed that the 0.5 ml of spore suspension (107 spore/ml) gave the best effective way to de-emulsifying, while 30 min. the best period appropriate to de-emulsifying.https://etj.uotechnology.edu.iq/article_105287_73e9d20106d18e7a5a7b2cf424dfb70f.pdfemulsificationactinomycetesnocardia sp
spellingShingle Salah H. Kalaf
Eman H. Gata
Amil A. Halob
Saad H. Khudhair
Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils
Engineering and Technology Journal
emulsification
actinomycetes
nocardia sp
title Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils
title_full Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils
title_fullStr Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils
title_full_unstemmed Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils
title_short Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils
title_sort production of biological de emulsification by bacterial isolates isolated from local soils
topic emulsification
actinomycetes
nocardia sp
url https://etj.uotechnology.edu.iq/article_105287_73e9d20106d18e7a5a7b2cf424dfb70f.pdf
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AT amilahalob productionofbiologicaldeemulsificationbybacterialisolatesisolatedfromlocalsoils
AT saadhkhudhair productionofbiologicaldeemulsificationbybacterialisolatesisolatedfromlocalsoils