Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils
The purpose of this research was to isolate local bacterial have the effectiveness of de-emulsification in liquid emulsion. Twenty local isolates of actinomycetes were isolated from soil contaminated with oil waste using mineral liquid medium at initial pH of 7 in the presence of oil waste (1%) as a...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Unviversity of Technology- Iraq
2014-06-01
|
Series: | Engineering and Technology Journal |
Subjects: | |
Online Access: | https://etj.uotechnology.edu.iq/article_105287_73e9d20106d18e7a5a7b2cf424dfb70f.pdf |
_version_ | 1797325750977691648 |
---|---|
author | Salah H. Kalaf Eman H. Gata Amil A. Halob Saad H. Khudhair |
author_facet | Salah H. Kalaf Eman H. Gata Amil A. Halob Saad H. Khudhair |
author_sort | Salah H. Kalaf |
collection | DOAJ |
description | The purpose of this research was to isolate local bacterial have the effectiveness of de-emulsification in liquid emulsion. Twenty local isolates of actinomycetes were isolated from soil contaminated with oil waste using mineral liquid medium at initial pH of 7 in the presence of oil waste (1%) as a source of carbon and incubated at 40 °C for 5 days . The primary screening of isolates was tested to de-emulsifying ability.Results indicate that the isolates ST3 , ST8 and ST15 were selected for theirhigh ability to de-emulsification. Secondary screening of selected isolates was estimated to deemulsification using the spore suspension . Results indicate that the ST8 isolate showed the highest de-emulsification activity, subsequently was identified and designated as Nocardia sp.. Studied the effects of both the spores number and period of exposure on the effectiveness of de-emulsification. The results showed that the 0.5 ml of spore suspension (107 spore/ml) gave the best effective way to de-emulsifying, while 30 min. the best period appropriate to de-emulsifying. |
first_indexed | 2024-03-08T06:14:00Z |
format | Article |
id | doaj.art-ddfd997d043e4d93bc94502cef205df3 |
institution | Directory Open Access Journal |
issn | 1681-6900 2412-0758 |
language | English |
last_indexed | 2024-03-08T06:14:00Z |
publishDate | 2014-06-01 |
publisher | Unviversity of Technology- Iraq |
record_format | Article |
series | Engineering and Technology Journal |
spelling | doaj.art-ddfd997d043e4d93bc94502cef205df32024-02-04T17:30:57ZengUnviversity of Technology- IraqEngineering and Technology Journal1681-69002412-07582014-06-01326B808510.30684/etj.32.6B.20105287Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local SoilsSalah H. KalafEman H. GataAmil A. HalobSaad H. KhudhairThe purpose of this research was to isolate local bacterial have the effectiveness of de-emulsification in liquid emulsion. Twenty local isolates of actinomycetes were isolated from soil contaminated with oil waste using mineral liquid medium at initial pH of 7 in the presence of oil waste (1%) as a source of carbon and incubated at 40 °C for 5 days . The primary screening of isolates was tested to de-emulsifying ability.Results indicate that the isolates ST3 , ST8 and ST15 were selected for theirhigh ability to de-emulsification. Secondary screening of selected isolates was estimated to deemulsification using the spore suspension . Results indicate that the ST8 isolate showed the highest de-emulsification activity, subsequently was identified and designated as Nocardia sp.. Studied the effects of both the spores number and period of exposure on the effectiveness of de-emulsification. The results showed that the 0.5 ml of spore suspension (107 spore/ml) gave the best effective way to de-emulsifying, while 30 min. the best period appropriate to de-emulsifying.https://etj.uotechnology.edu.iq/article_105287_73e9d20106d18e7a5a7b2cf424dfb70f.pdfemulsificationactinomycetesnocardia sp |
spellingShingle | Salah H. Kalaf Eman H. Gata Amil A. Halob Saad H. Khudhair Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils Engineering and Technology Journal emulsification actinomycetes nocardia sp |
title | Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils |
title_full | Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils |
title_fullStr | Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils |
title_full_unstemmed | Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils |
title_short | Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils |
title_sort | production of biological de emulsification by bacterial isolates isolated from local soils |
topic | emulsification actinomycetes nocardia sp |
url | https://etj.uotechnology.edu.iq/article_105287_73e9d20106d18e7a5a7b2cf424dfb70f.pdf |
work_keys_str_mv | AT salahhkalaf productionofbiologicaldeemulsificationbybacterialisolatesisolatedfromlocalsoils AT emanhgata productionofbiologicaldeemulsificationbybacterialisolatesisolatedfromlocalsoils AT amilahalob productionofbiologicaldeemulsificationbybacterialisolatesisolatedfromlocalsoils AT saadhkhudhair productionofbiologicaldeemulsificationbybacterialisolatesisolatedfromlocalsoils |