Growth of Methylobacterium organophilum in Methanol for the Simultaneous Production of Single-Cell Protein and Metabolites of Interest
Research background. This study aims to monitor the growth of the methylotrophic bacteria Methylobacterium organophilum in a culture medium with methanol as a carbon source and to verify the production of unicellular proteins and other biomolecules, such as carotenoids, exopolysaccharides and polyhy...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2022-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | https://hrcak.srce.hr/file/412075 |
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| author | Ana Cristina Pantoja Simões Rodrigo Pimentel Fernandes Maysa Silva Barreto Gabriela Bouça Marques da Costa Mateus Gomes de Godoy Denise Maria Guimarães Freire Nei Pereira |
| author_facet | Ana Cristina Pantoja Simões Rodrigo Pimentel Fernandes Maysa Silva Barreto Gabriela Bouça Marques da Costa Mateus Gomes de Godoy Denise Maria Guimarães Freire Nei Pereira |
| author_sort | Ana Cristina Pantoja Simões |
| collection | DOAJ |
| description | Research background. This study aims to monitor the growth of the methylotrophic bacteria Methylobacterium organophilum in a culture medium with methanol as a carbon source and to verify the production of unicellular proteins and other biomolecules, such as carotenoids, exopolysaccharides and polyhydroxyalkanoates, making them more attractive as animal feed.
Experimental approach. Bacterial growth was studied in shake flasks using different carbon/nitrogen (C:N) ratios to determine their best ratio for achieving the highest volumetric productivity of cells and substrate consumption rate. This optimal parameter was further used in a fed-batch operating bioreactor system to define the kinetic profile of cell growth. Methanol consumption was measured by HPLC analysis and the extracted pigments were analyzed by liquid chromatography/mass spectrometry. Chemical composition and rheological properties of the produced exopolysaccharides were also determined.
Results and conclusions. The best experimental parameters were verified using an initial methanol concentration of 7 g/L in the culture medium. The same initial substrate concentration was used in the fed-batch operation and after 60 h of cultivation 5 g/L of biomass were obtained. The accumulation of carotenoids associated with cell growth was monitored, reaching a concentration of 1.6 mg/L at the end of the process. These pigments were then analyzed and characterized as a set of xanthophylls (oxidized carotenoids). In addition, two other product types were identified during the fed-batch operation: exopolysaccharides, which reached a concentration of 8.9 g/L at the end of the cultivation, and an intracellular granular structure that was detected by transmission electron microscopy (TEM), suggesting the accumulation of polyhydroxyalkanoate (PHA), most likely polyhydroxybutyrate.
Novelty and scientific contribution. Methylobacterium organophilum demonstrated a unique ability to produce compounds of commercial interest. The distinct metabolic diversity of this bacterium makes room for its use in biorefineries. |
| first_indexed | 2024-04-24T09:10:57Z |
| format | Article |
| id | doaj.art-ddffe14883424e1dbe7231d968328dc5 |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-04-24T09:10:57Z |
| publishDate | 2022-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-ddffe14883424e1dbe7231d968328dc52024-04-15T17:59:42ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062022-01-0160333834910.17113/ftb.60.03.22.7372Growth of Methylobacterium organophilum in Methanol for the Simultaneous Production of Single-Cell Protein and Metabolites of InterestAna Cristina Pantoja Simões0Rodrigo Pimentel Fernandes1Maysa Silva Barreto2Gabriela Bouça Marques da Costa3Mateus Gomes de Godoy4Denise Maria Guimarães Freire5Nei Pereira6Laboratories of Bioprocess Development, School of Chemistry, Federal University of Rio de Janeiro, RJ 21941-972, BrazilLaboratories of Bioprocess Development, School of Chemistry, Federal University of Rio de Janeiro, RJ 21941-972, BrazilMicrobial Biotechnology Laboratory, Institute of Chemistry, Exact and Natural Sciences Center of the Federal University of Rio de Janeiro, RJ 21941-972, BrazilMicrobial Biotechnology Laboratory, Institute of Chemistry, Exact and Natural Sciences Center of the Federal University of Rio de Janeiro, RJ 21941-972, BrazilLaboratory of Biotechnology and Microbial Ecology, Institute of Microbiology, Federal University of Rio de Janeiro, RJ 21941-902, BrazilMicrobial Biotechnology Laboratory, Institute of Chemistry, Exact and Natural Sciences Center of the Federal University of Rio de Janeiro, RJ 21941-972, BrazilLaboratories of Bioprocess Development, School of Chemistry, Federal University of Rio de Janeiro, RJ 21941-972, BrazilResearch background. This study aims to monitor the growth of the methylotrophic bacteria Methylobacterium organophilum in a culture medium with methanol as a carbon source and to verify the production of unicellular proteins and other biomolecules, such as carotenoids, exopolysaccharides and polyhydroxyalkanoates, making them more attractive as animal feed. Experimental approach. Bacterial growth was studied in shake flasks using different carbon/nitrogen (C:N) ratios to determine their best ratio for achieving the highest volumetric productivity of cells and substrate consumption rate. This optimal parameter was further used in a fed-batch operating bioreactor system to define the kinetic profile of cell growth. Methanol consumption was measured by HPLC analysis and the extracted pigments were analyzed by liquid chromatography/mass spectrometry. Chemical composition and rheological properties of the produced exopolysaccharides were also determined. Results and conclusions. The best experimental parameters were verified using an initial methanol concentration of 7 g/L in the culture medium. The same initial substrate concentration was used in the fed-batch operation and after 60 h of cultivation 5 g/L of biomass were obtained. The accumulation of carotenoids associated with cell growth was monitored, reaching a concentration of 1.6 mg/L at the end of the process. These pigments were then analyzed and characterized as a set of xanthophylls (oxidized carotenoids). In addition, two other product types were identified during the fed-batch operation: exopolysaccharides, which reached a concentration of 8.9 g/L at the end of the cultivation, and an intracellular granular structure that was detected by transmission electron microscopy (TEM), suggesting the accumulation of polyhydroxyalkanoate (PHA), most likely polyhydroxybutyrate. Novelty and scientific contribution. Methylobacterium organophilum demonstrated a unique ability to produce compounds of commercial interest. The distinct metabolic diversity of this bacterium makes room for its use in biorefineries.https://hrcak.srce.hr/file/412075single cell proteinmethylotrophic bacteriummethanol consumption |
| spellingShingle | Ana Cristina Pantoja Simões Rodrigo Pimentel Fernandes Maysa Silva Barreto Gabriela Bouça Marques da Costa Mateus Gomes de Godoy Denise Maria Guimarães Freire Nei Pereira Growth of Methylobacterium organophilum in Methanol for the Simultaneous Production of Single-Cell Protein and Metabolites of Interest Food Technology and Biotechnology single cell protein methylotrophic bacterium methanol consumption |
| title | Growth of Methylobacterium organophilum in Methanol for the Simultaneous Production of Single-Cell Protein and Metabolites of Interest |
| title_full | Growth of Methylobacterium organophilum in Methanol for the Simultaneous Production of Single-Cell Protein and Metabolites of Interest |
| title_fullStr | Growth of Methylobacterium organophilum in Methanol for the Simultaneous Production of Single-Cell Protein and Metabolites of Interest |
| title_full_unstemmed | Growth of Methylobacterium organophilum in Methanol for the Simultaneous Production of Single-Cell Protein and Metabolites of Interest |
| title_short | Growth of Methylobacterium organophilum in Methanol for the Simultaneous Production of Single-Cell Protein and Metabolites of Interest |
| title_sort | growth of methylobacterium organophilum in methanol for the simultaneous production of single cell protein and metabolites of interest |
| topic | single cell protein methylotrophic bacterium methanol consumption |
| url | https://hrcak.srce.hr/file/412075 |
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