Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts
Bacterial production of siderophores may involve specific genes related to nonribosomal peptide and polyketide biosynthesis, which have not been fully identified in the genome of Xylella fastidiosa strain 9a5c. However, a search for siderophore-related genes in strain 9a5c indicated five membrane re...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2006-01-01
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100026 |
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| author | Flávia Teresa Hansen Pacheco Maria Estela Silva-Stenico Augusto Etchegaray José Elias Gomes Emanuel Carrilho Siu Mui Tsai |
| author_facet | Flávia Teresa Hansen Pacheco Maria Estela Silva-Stenico Augusto Etchegaray José Elias Gomes Emanuel Carrilho Siu Mui Tsai |
| author_sort | Flávia Teresa Hansen Pacheco |
| collection | DOAJ |
| description | Bacterial production of siderophores may involve specific genes related to nonribosomal peptide and polyketide biosynthesis, which have not been fully identified in the genome of Xylella fastidiosa strain 9a5c. However, a search for siderophore-related genes in strain 9a5c indicated five membrane receptors, including siderophore, ferrichrome-iron and hemin receptors. All these biomolecules are thought to be associated with iron transport and utilization. Eighty isolates obtained from citrus orchards containing trees that developed citrus variegated chlorosis (CVC) were screened for siderophore production. The results demonstrated that only 10 of the isolates did not produce siderophores. Additional strains obtained from coffee, almond, mulberry, elm, ragweed, periwinkle and grape also infected by X. fastidiosa were also shown by the chromeazurol bioassay to produce siderophores. In order to correlate siderophore production with the presence of siderophore-related genes, a polymerase chain reaction (PCR) was developed using specific primers for the catechol-type ferric enterobactin receptor (pfeA) and the hydroxamate-type ferrisiderophore receptor (fiuA) genes of strain 9a5c. The PCR results confirmed our hypothesis by demonstrating that amplification products were detected in all strains except for those isolates that did not produce siderophores. |
| first_indexed | 2024-12-19T08:02:01Z |
| format | Article |
| id | doaj.art-de03ec104b4f49a0ba594cfa0292c5ed |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-19T08:02:01Z |
| publishDate | 2006-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-de03ec104b4f49a0ba594cfa0292c5ed2022-12-21T20:29:50ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852006-01-0129113714110.1590/S1415-47572006000100026Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hostsFlávia Teresa Hansen PachecoMaria Estela Silva-StenicoAugusto EtchegarayJosé Elias GomesEmanuel CarrilhoSiu Mui TsaiBacterial production of siderophores may involve specific genes related to nonribosomal peptide and polyketide biosynthesis, which have not been fully identified in the genome of Xylella fastidiosa strain 9a5c. However, a search for siderophore-related genes in strain 9a5c indicated five membrane receptors, including siderophore, ferrichrome-iron and hemin receptors. All these biomolecules are thought to be associated with iron transport and utilization. Eighty isolates obtained from citrus orchards containing trees that developed citrus variegated chlorosis (CVC) were screened for siderophore production. The results demonstrated that only 10 of the isolates did not produce siderophores. Additional strains obtained from coffee, almond, mulberry, elm, ragweed, periwinkle and grape also infected by X. fastidiosa were also shown by the chromeazurol bioassay to produce siderophores. In order to correlate siderophore production with the presence of siderophore-related genes, a polymerase chain reaction (PCR) was developed using specific primers for the catechol-type ferric enterobactin receptor (pfeA) and the hydroxamate-type ferrisiderophore receptor (fiuA) genes of strain 9a5c. The PCR results confirmed our hypothesis by demonstrating that amplification products were detected in all strains except for those isolates that did not produce siderophores.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100026citrus variegated chlorosisplant pathogeniron-transportationpyoverdineenterobactinpolyketide synthasepeptide synthetase |
| spellingShingle | Flávia Teresa Hansen Pacheco Maria Estela Silva-Stenico Augusto Etchegaray José Elias Gomes Emanuel Carrilho Siu Mui Tsai Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts Genetics and Molecular Biology citrus variegated chlorosis plant pathogen iron-transportation pyoverdine enterobactin polyketide synthase peptide synthetase |
| title | Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts |
| title_full | Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts |
| title_fullStr | Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts |
| title_full_unstemmed | Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts |
| title_short | Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts |
| title_sort | specific amplification of iron receptor genes in xylella fastidiosa strains from different hosts |
| topic | citrus variegated chlorosis plant pathogen iron-transportation pyoverdine enterobactin polyketide synthase peptide synthetase |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100026 |
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