Effect of extender and storage temperature on sperm motility parameters of liquid ram semen
The objective of this work was to compare the effect of eight extenders (Skim milk, INRA96®, Tris Eggs Yolk, Triladyl®, Ovipro®, Andromed®, Optixcel®,and Duragen®) on ram sperm motion parameters. Two experiments were carried out to determine the effect of extenders on ram sperm preservation at 15°C...
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| Format: | Article |
| Language: | English |
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Institut Agronomique et Vétérinaire Hassan II
2018-06-01
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| Series: | Revue Marocaine des Sciences Agronomiques et Vétérinaires |
| Subjects: | |
| Online Access: | http://www.agrimaroc.org/index.php/Actes_IAVH2/article/view/601 |
| _version_ | 1818048106233593856 |
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| author | A. BEN MOULA L. ALLAI A. BADI K. EL KHALIL B. EL AMIRI |
| author_facet | A. BEN MOULA L. ALLAI A. BADI K. EL KHALIL B. EL AMIRI |
| author_sort | A. BEN MOULA |
| collection | DOAJ |
| description | The objective of this work was to compare the effect of eight extenders (Skim milk, INRA96®, Tris Eggs Yolk, Triladyl®, Ovipro®, Andromed®, Optixcel®,and Duragen®) on ram sperm motion parameters. Two experiments were carried out to determine the effect of extenders on ram sperm preservation at 15°C (Experiment 1), or at 5°C (Experiment 2). Semen was collected using an artificial vagina weekly for 10 weeks from two breeds (INRA180, n=4, on Monday and Boujaâd, n=4, on Friday). Aliquots were extended in each extender to a final concentration of 0.8 × 109 spermatozoa/ml and stored at 15°Cor 5°C for 24 h. Motility and kinematic parameters assessment were performed using a CASA system. Sperm motility and kinematic parameters decreased significantly over time of storage in all extenders and storage temperature. The overall CASA parameters were affected by extenders in different manners depending on breed and storage temperature. Skim milk, Duragen®, and INRA96® provided the best CASA results. |
| first_indexed | 2024-12-10T10:16:24Z |
| format | Article |
| id | doaj.art-de0be685140c4007915becdf2ccc864d |
| institution | Directory Open Access Journal |
| issn | 2028-991X 2550-4401 |
| language | English |
| last_indexed | 2024-12-10T10:16:24Z |
| publishDate | 2018-06-01 |
| publisher | Institut Agronomique et Vétérinaire Hassan II |
| record_format | Article |
| series | Revue Marocaine des Sciences Agronomiques et Vétérinaires |
| spelling | doaj.art-de0be685140c4007915becdf2ccc864d2022-12-22T01:53:00ZengInstitut Agronomique et Vétérinaire Hassan IIRevue Marocaine des Sciences Agronomiques et Vétérinaires2028-991X2550-44012018-06-0162211219Effect of extender and storage temperature on sperm motility parameters of liquid ram semenA. BEN MOULA0L. ALLAI1A. BADI2K. EL KHALIL3B. EL AMIRI4Faculté des Sciences et Techniques, Université Hassan 1er, BP 577, 26000 Settat, MoroccoINRA-Centre Régional de la Recherche Agronomique de Settat, BP589, Settat, MoroccoINRA-Centre Régional de la Recherche Agronomique de Settat, BP589, Settat, MoroccoINRA-Centre Régional de la Recherche Agronomique de Settat, BP589, Settat, MoroccoINRA-Centre Régional de la Recherche Agronomique de Settat, BP589, Settat, MoroccoThe objective of this work was to compare the effect of eight extenders (Skim milk, INRA96®, Tris Eggs Yolk, Triladyl®, Ovipro®, Andromed®, Optixcel®,and Duragen®) on ram sperm motion parameters. Two experiments were carried out to determine the effect of extenders on ram sperm preservation at 15°C (Experiment 1), or at 5°C (Experiment 2). Semen was collected using an artificial vagina weekly for 10 weeks from two breeds (INRA180, n=4, on Monday and Boujaâd, n=4, on Friday). Aliquots were extended in each extender to a final concentration of 0.8 × 109 spermatozoa/ml and stored at 15°Cor 5°C for 24 h. Motility and kinematic parameters assessment were performed using a CASA system. Sperm motility and kinematic parameters decreased significantly over time of storage in all extenders and storage temperature. The overall CASA parameters were affected by extenders in different manners depending on breed and storage temperature. Skim milk, Duragen®, and INRA96® provided the best CASA results.http://www.agrimaroc.org/index.php/Actes_IAVH2/article/view/601Semenextendermotilitykinematic parametersstorage temperatureram breed |
| spellingShingle | A. BEN MOULA L. ALLAI A. BADI K. EL KHALIL B. EL AMIRI Effect of extender and storage temperature on sperm motility parameters of liquid ram semen Revue Marocaine des Sciences Agronomiques et Vétérinaires Semen extender motility kinematic parameters storage temperature ram breed |
| title | Effect of extender and storage temperature on sperm motility parameters of liquid ram semen |
| title_full | Effect of extender and storage temperature on sperm motility parameters of liquid ram semen |
| title_fullStr | Effect of extender and storage temperature on sperm motility parameters of liquid ram semen |
| title_full_unstemmed | Effect of extender and storage temperature on sperm motility parameters of liquid ram semen |
| title_short | Effect of extender and storage temperature on sperm motility parameters of liquid ram semen |
| title_sort | effect of extender and storage temperature on sperm motility parameters of liquid ram semen |
| topic | Semen extender motility kinematic parameters storage temperature ram breed |
| url | http://www.agrimaroc.org/index.php/Actes_IAVH2/article/view/601 |
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