HPLC-MS, GC and NMR Profiling of Bioactive Lipids of Human Milk and Milk of Dairy Animals (Cow, Sheep, Goat, Buffalo, Camel, Red Deer)
For non-bovine milks, information regarding bioactive lipids is fragmented, unreliable or unavailable. The purpose of the current study was to analyse bioactive lipids in the milk of dairy animals using modern analytical methods to achieve the most reliable results. Bioactive lipids in human milk we...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Separations |
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| Online Access: | https://www.mdpi.com/2297-8739/9/6/145 |
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| author | Kirill Lagutin Andrew MacKenzie Stephen Bloor Dawn Scott Mikhail Vyssotski |
| author_facet | Kirill Lagutin Andrew MacKenzie Stephen Bloor Dawn Scott Mikhail Vyssotski |
| author_sort | Kirill Lagutin |
| collection | DOAJ |
| description | For non-bovine milks, information regarding bioactive lipids is fragmented, unreliable or unavailable. The purpose of the current study was to analyse bioactive lipids in the milk of dairy animals using modern analytical methods to achieve the most reliable results. Bioactive lipids in human milk were also analysed and used as a reference. A suite of modern analytical methods was employed, namely High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS), Gas Chromatography (GC) and Nuclear Magnetic Resonance (NMR). The total lipid content was determined, and phospholipid, fatty acid, neutral glycosphingolipids and ganglioside (GM3 and GD3) levels were measured. Lipid classes in selected milks were reliably characterised for the first time, including gangliosides in deer, camel and sheep; cerebrosides in deer, camel and buffalo; plasmalogens in deer, buffalo and goat and phospholipids in deer. Our study demonstrated the advantage of utilising a range of analytical techniques in order to characterise a diverse set of bioactive lipids. |
| first_indexed | 2024-03-09T22:31:19Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-09T22:31:19Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-de0e77e5396f43dfb7f6239c8681a96d2023-11-23T18:57:03ZengMDPI AGSeparations2297-87392022-06-019614510.3390/separations9060145HPLC-MS, GC and NMR Profiling of Bioactive Lipids of Human Milk and Milk of Dairy Animals (Cow, Sheep, Goat, Buffalo, Camel, Red Deer)Kirill Lagutin0Andrew MacKenzie1Stephen Bloor2Dawn Scott3Mikhail Vyssotski4Callaghan Innovation, P.O. Box 31310, Lower Hutt 5040, New ZealandCallaghan Innovation, P.O. Box 31310, Lower Hutt 5040, New ZealandCallaghan Innovation, P.O. Box 31310, Lower Hutt 5040, New ZealandCallaghan Innovation, P.O. Box 31310, Lower Hutt 5040, New ZealandCallaghan Innovation, P.O. Box 31310, Lower Hutt 5040, New ZealandFor non-bovine milks, information regarding bioactive lipids is fragmented, unreliable or unavailable. The purpose of the current study was to analyse bioactive lipids in the milk of dairy animals using modern analytical methods to achieve the most reliable results. Bioactive lipids in human milk were also analysed and used as a reference. A suite of modern analytical methods was employed, namely High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS), Gas Chromatography (GC) and Nuclear Magnetic Resonance (NMR). The total lipid content was determined, and phospholipid, fatty acid, neutral glycosphingolipids and ganglioside (GM3 and GD3) levels were measured. Lipid classes in selected milks were reliably characterised for the first time, including gangliosides in deer, camel and sheep; cerebrosides in deer, camel and buffalo; plasmalogens in deer, buffalo and goat and phospholipids in deer. Our study demonstrated the advantage of utilising a range of analytical techniques in order to characterise a diverse set of bioactive lipids.https://www.mdpi.com/2297-8739/9/6/145milklipidsNMRHPLCphospholipidsfatty acids |
| spellingShingle | Kirill Lagutin Andrew MacKenzie Stephen Bloor Dawn Scott Mikhail Vyssotski HPLC-MS, GC and NMR Profiling of Bioactive Lipids of Human Milk and Milk of Dairy Animals (Cow, Sheep, Goat, Buffalo, Camel, Red Deer) Separations milk lipids NMR HPLC phospholipids fatty acids |
| title | HPLC-MS, GC and NMR Profiling of Bioactive Lipids of Human Milk and Milk of Dairy Animals (Cow, Sheep, Goat, Buffalo, Camel, Red Deer) |
| title_full | HPLC-MS, GC and NMR Profiling of Bioactive Lipids of Human Milk and Milk of Dairy Animals (Cow, Sheep, Goat, Buffalo, Camel, Red Deer) |
| title_fullStr | HPLC-MS, GC and NMR Profiling of Bioactive Lipids of Human Milk and Milk of Dairy Animals (Cow, Sheep, Goat, Buffalo, Camel, Red Deer) |
| title_full_unstemmed | HPLC-MS, GC and NMR Profiling of Bioactive Lipids of Human Milk and Milk of Dairy Animals (Cow, Sheep, Goat, Buffalo, Camel, Red Deer) |
| title_short | HPLC-MS, GC and NMR Profiling of Bioactive Lipids of Human Milk and Milk of Dairy Animals (Cow, Sheep, Goat, Buffalo, Camel, Red Deer) |
| title_sort | hplc ms gc and nmr profiling of bioactive lipids of human milk and milk of dairy animals cow sheep goat buffalo camel red deer |
| topic | milk lipids NMR HPLC phospholipids fatty acids |
| url | https://www.mdpi.com/2297-8739/9/6/145 |
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