First report of the multiresistance gene cfr in Pasteurella multocida strains of avian origin from China
Objectives: The aim of this study was to investigate the presence and genetic environment of the multiresistance gene cfr gene in Pasteurella multocida of avian origin from China. Methods: A total of 113 P. multocida isolates were collected from sick poultries (ducks, chickens and geese) from 2003 t...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716520302484 |
| _version_ | 1818921649777410048 |
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| author | Hongmei Chen Hui Deng Longfei Cheng Rongchang Liu Guanghua Fu Shaohua Shi Chunhe Wan Qiuling Fu Yu Huang Xiaohong Huang |
| author_facet | Hongmei Chen Hui Deng Longfei Cheng Rongchang Liu Guanghua Fu Shaohua Shi Chunhe Wan Qiuling Fu Yu Huang Xiaohong Huang |
| author_sort | Hongmei Chen |
| collection | DOAJ |
| description | Objectives: The aim of this study was to investigate the presence and genetic environment of the multiresistance gene cfr gene in Pasteurella multocida of avian origin from China. Methods: A total of 113 P. multocida isolates were collected from sick poultries (ducks, chickens and geese) from 2003 to 2016 in Southern China and were screened for the presence of the cfr gene by PCR. The cfr-carrying P. multocida strains were subjected to antimicrobial susceptibility testing, S1 nuclease PFGE and Southern blot hybridisation, conjugative transfer and analysis of genetic environment of the cfr gene. Results: Among 113 P. multocida isolates, strains FJ6671 and FJ6683 from Muscovy duck harboured the cfr gene and presented a multiresistant phenotype. The cfr gene in the two strains was located on an ∼40-kb conjugative plasmid in different genetic environments, including ISApl12–cfr–IS26 and IS26–cfr–IS256. Conclusions: These results demonstrate plasmid-carried cfr in P. multocida and suggest that transposition and homologous recombination mediated by IS26, ISApl1 and IS256 might have played an important role in transfer of the cfr gene in P. multocida. To the best of our knowledge, this is the first report of the cfr gene in P. multocida. Active and ongoing surveillance of cfr in P. multocida is urgently warranted. |
| first_indexed | 2024-12-20T01:41:00Z |
| format | Article |
| id | doaj.art-de1223e753554e0dafa807a2adbd809b |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-20T01:41:00Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-de1223e753554e0dafa807a2adbd809b2022-12-21T19:57:53ZengElsevierJournal of Global Antimicrobial Resistance2213-71652020-12-0123251255First report of the multiresistance gene cfr in Pasteurella multocida strains of avian origin from ChinaHongmei Chen0Hui Deng1Longfei Cheng2Rongchang Liu3Guanghua Fu4Shaohua Shi5Chunhe Wan6Qiuling Fu7Yu Huang8Xiaohong Huang9College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; Fujian Animal Diseases Control Technology Center, Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, PR ChinaFujian Provincial Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Sciences (College of Bee Sciences), Fujian Agriculture and Forestry University, Fuzhou 350002, PR ChinaFujian Animal Diseases Control Technology Center, Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, PR ChinaFujian Animal Diseases Control Technology Center, Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, PR ChinaFujian Animal Diseases Control Technology Center, Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, PR ChinaFujian Animal Diseases Control Technology Center, Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, PR ChinaFujian Animal Diseases Control Technology Center, Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, PR ChinaFujian Animal Diseases Control Technology Center, Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, PR ChinaFujian Animal Diseases Control Technology Center, Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, PR China; Corresponding author. Present address: Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, PR China.Fujian Provincial Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Sciences (College of Bee Sciences), Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; Corresponding author. Present address: College of Animal Science (College of Bee Sciences), Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.Objectives: The aim of this study was to investigate the presence and genetic environment of the multiresistance gene cfr gene in Pasteurella multocida of avian origin from China. Methods: A total of 113 P. multocida isolates were collected from sick poultries (ducks, chickens and geese) from 2003 to 2016 in Southern China and were screened for the presence of the cfr gene by PCR. The cfr-carrying P. multocida strains were subjected to antimicrobial susceptibility testing, S1 nuclease PFGE and Southern blot hybridisation, conjugative transfer and analysis of genetic environment of the cfr gene. Results: Among 113 P. multocida isolates, strains FJ6671 and FJ6683 from Muscovy duck harboured the cfr gene and presented a multiresistant phenotype. The cfr gene in the two strains was located on an ∼40-kb conjugative plasmid in different genetic environments, including ISApl12–cfr–IS26 and IS26–cfr–IS256. Conclusions: These results demonstrate plasmid-carried cfr in P. multocida and suggest that transposition and homologous recombination mediated by IS26, ISApl1 and IS256 might have played an important role in transfer of the cfr gene in P. multocida. To the best of our knowledge, this is the first report of the cfr gene in P. multocida. Active and ongoing surveillance of cfr in P. multocida is urgently warranted.http://www.sciencedirect.com/science/article/pii/S2213716520302484Pasteurella multocidacfrResistance geneFlorfenicolISApl1IS256 |
| spellingShingle | Hongmei Chen Hui Deng Longfei Cheng Rongchang Liu Guanghua Fu Shaohua Shi Chunhe Wan Qiuling Fu Yu Huang Xiaohong Huang First report of the multiresistance gene cfr in Pasteurella multocida strains of avian origin from China Journal of Global Antimicrobial Resistance Pasteurella multocida cfr Resistance gene Florfenicol ISApl1 IS256 |
| title | First report of the multiresistance gene cfr in Pasteurella multocida strains of avian origin from China |
| title_full | First report of the multiresistance gene cfr in Pasteurella multocida strains of avian origin from China |
| title_fullStr | First report of the multiresistance gene cfr in Pasteurella multocida strains of avian origin from China |
| title_full_unstemmed | First report of the multiresistance gene cfr in Pasteurella multocida strains of avian origin from China |
| title_short | First report of the multiresistance gene cfr in Pasteurella multocida strains of avian origin from China |
| title_sort | first report of the multiresistance gene cfr in pasteurella multocida strains of avian origin from china |
| topic | Pasteurella multocida cfr Resistance gene Florfenicol ISApl1 IS256 |
| url | http://www.sciencedirect.com/science/article/pii/S2213716520302484 |
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