Comparative Evaluation of Antimicrobial Efficacy of Chlorhexidine, MTAD and Chitosan as Root Canal Irrigant against Enterococcus faecalis
Introduction: Endodontic irrigants play a role in disinfection of root canal. Inspite of wider options on selection of irrigant, there is always a search on ideal antibacterial irrigant against recurrent infections. Aim: To comparatively evaluate the antibacterial efficacy of chlorhexidine, Mix...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2018-12-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/12368/36191_CE[Ra1]_F(SL)_PF1(A_SHU)_PFA(A_SHU)_PB(NC)_PN(SL).pdf |
| Summary: | Introduction: Endodontic irrigants play a role in disinfection
of root canal. Inspite of wider options on selection of irrigant,
there is always a search on ideal antibacterial irrigant against
recurrent infections.
Aim: To comparatively evaluate the antibacterial efficacy of
chlorhexidine, Mixture of Tetracycline, Acid and Detergent
(MTAD) and Chitosan against Enterococcus faecalis when used
as a root canal irrigant.
Materials and Methods: The bacterial E. faecalis culture
was grown overnight in Brain Heart Infusion (BHI) broth and
inoculated in Mueller-Hinton agar plates. The root canal irrigants
were divided into four groups as follows. (Group I)-4% Sodium
hypochlorite solutions; (Group II)-2% Chlorhexidine (CHX);
(Group III)-MTAD; (Group IV)-2% Chitosan. Bacterial inhibition
was assessed using agar well diffusion method. All four study
irrigants were added to respective wells in agar plate (n=10) and
incubated at 37°C for 24 hour. Diameter of bacterial inhibition
zone around each well was recorded. The results obtained
were statistically evaluated using one-way ANOVA test and the
intergroup comparison was done using student’s t-test.
Results: All the materials had statistically significant difference
in zone of bacterial inhibition when compared to other materials.
Based on the mean diameters, 4% sodium hypochlorite
had the least zone of inhibition and MTAD had the highest
zone of inhibition. 2% Chitosan polymer had greater zone of
inhibition than 4% sodium hypochlorite, but less than 2% CHX
and MTAD.
Conclusion: MTAD showed the highest antibacterial efficacy
against Enterococcus faecalis followed by CHX and Chitosan. |
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| ISSN: | 2249-782X 0973-709X |