The role of intracellular signaling in the stripe formation in engineered Escherichia coli populations.
Recent experiments showed that engineered Escherichia coli colonies grow and self-organize into periodic stripes with high and low cell densities in semi-solid agar. The stripes develop sequentially behind a radially propagating colony front, similar to the formation of many other periodic patterns...
| _version_ | 1826579520871727104 |
|---|---|
| author | Xiaoru Xue Chuan Xue Min Tang |
| author_facet | Xiaoru Xue Chuan Xue Min Tang |
| author_sort | Xiaoru Xue |
| collection | DOAJ |
| description | Recent experiments showed that engineered Escherichia coli colonies grow and self-organize into periodic stripes with high and low cell densities in semi-solid agar. The stripes develop sequentially behind a radially propagating colony front, similar to the formation of many other periodic patterns in nature. These bacteria were created by genetically coupling the intracellular chemotaxis pathway of wild-type cells with a quorum sensing module through the protein CheZ. In this paper, we develop multiscale models to investigate how this intracellular pathway affects stripe formation. We first develop a detailed hybrid model that treats each cell as an individual particle and incorporates intracellular signaling via an internal ODE system. To overcome the computational cost of the hybrid model caused by the large number of cells involved, we next derive a mean-field PDE model from the hybrid model using asymptotic analysis. We show that this analysis is justified by the tight agreement between the PDE model and the hybrid model in 1D simulations. Numerical simulations of the PDE model in 2D with radial symmetry agree with experimental data semi-quantitatively. Finally, we use the PDE model to make a number of testable predictions on how the stripe patterns depend on cell-level parameters, including cell speed, cell doubling time and the turnover rate of intracellular CheZ. |
| first_indexed | 2024-12-13T04:02:02Z |
| format | Article |
| id | doaj.art-de1cd63d503e4b7cb531d4d7e9361002 |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2025-03-14T14:19:35Z |
| publishDate | 2018-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-de1cd63d503e4b7cb531d4d7e93610022025-02-27T05:31:26ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582018-06-01146e100617810.1371/journal.pcbi.1006178The role of intracellular signaling in the stripe formation in engineered Escherichia coli populations.Xiaoru XueChuan XueMin TangRecent experiments showed that engineered Escherichia coli colonies grow and self-organize into periodic stripes with high and low cell densities in semi-solid agar. The stripes develop sequentially behind a radially propagating colony front, similar to the formation of many other periodic patterns in nature. These bacteria were created by genetically coupling the intracellular chemotaxis pathway of wild-type cells with a quorum sensing module through the protein CheZ. In this paper, we develop multiscale models to investigate how this intracellular pathway affects stripe formation. We first develop a detailed hybrid model that treats each cell as an individual particle and incorporates intracellular signaling via an internal ODE system. To overcome the computational cost of the hybrid model caused by the large number of cells involved, we next derive a mean-field PDE model from the hybrid model using asymptotic analysis. We show that this analysis is justified by the tight agreement between the PDE model and the hybrid model in 1D simulations. Numerical simulations of the PDE model in 2D with radial symmetry agree with experimental data semi-quantitatively. Finally, we use the PDE model to make a number of testable predictions on how the stripe patterns depend on cell-level parameters, including cell speed, cell doubling time and the turnover rate of intracellular CheZ.https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pcbi.1006178/2/pcbi.1006178.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210222%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210222T172104Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=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 |
| spellingShingle | Xiaoru Xue Chuan Xue Min Tang The role of intracellular signaling in the stripe formation in engineered Escherichia coli populations. PLoS Computational Biology |
| title | The role of intracellular signaling in the stripe formation in engineered Escherichia coli populations. |
| title_full | The role of intracellular signaling in the stripe formation in engineered Escherichia coli populations. |
| title_fullStr | The role of intracellular signaling in the stripe formation in engineered Escherichia coli populations. |
| title_full_unstemmed | The role of intracellular signaling in the stripe formation in engineered Escherichia coli populations. |
| title_short | The role of intracellular signaling in the stripe formation in engineered Escherichia coli populations. |
| title_sort | role of intracellular signaling in the stripe formation in engineered escherichia coli populations |
| url | https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pcbi.1006178/2/pcbi.1006178.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210222%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210222T172104Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=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 |
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