Autophagy enhances the replication of Peste des petits ruminants virus and inhibits caspase-dependent apoptosis in vitro
Peste des petits ruminants (PPR) is an acute and highly contagious disease in small ruminants that causes significant economic losses in developing countries. An increasing number of studies have demonstrated that both autophagy and apoptosis are important cellular mechanisms for maintaining homeost...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2018-12-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21505594.2018.1496776 |
| _version_ | 1811260948214710272 |
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| author | Bo Yang Qinghong Xue Xuefeng Qi Xueping Wang Peilong Jia Shuying Chen Ting Wang Tianxia Xue Jingyu Wang |
| author_facet | Bo Yang Qinghong Xue Xuefeng Qi Xueping Wang Peilong Jia Shuying Chen Ting Wang Tianxia Xue Jingyu Wang |
| author_sort | Bo Yang |
| collection | DOAJ |
| description | Peste des petits ruminants (PPR) is an acute and highly contagious disease in small ruminants that causes significant economic losses in developing countries. An increasing number of studies have demonstrated that both autophagy and apoptosis are important cellular mechanisms for maintaining homeostasis, and they participate in the host response to pathogens. However, the crosstalk between apoptosis and autophagy in host cells during PPRV infection has not been clarified. In this study, autophagy was induced upon virus infection in caprine endometrial epithelial cells (EECs), as determined by the appearance of double- and single-membrane autophagy-like vesicles, LC3-I/LC3-II conversion, and p62 degradation. We also found that PPRV infection triggered a complete autophagic response, most likely mediated by the non-structural protein C and nucleoprotein N. Moreover, our results suggest that autophagy not only promotes the replication of PPRV in EECs but also provides a potential mechanism for inhibiting PPRV-induced apoptosis. Inhibiting autophagosome formation by wortmannin and knocking down the essential autophagic proteins Beclin-1 and ATG7 induces caspase-dependent apoptosis in EECs in PPRV infection. However, inhibiting autophagosome and lysosome fusion by NH4Cl and chloroquine did not increase the number of apoptotic cells. Collectively, these data are the first to indicate that PPRV-induced autophagy inhibits caspase-dependent apoptosis and thus contributes to the enhancement of viral replication and maturity in host cells. |
| first_indexed | 2024-04-12T18:55:12Z |
| format | Article |
| id | doaj.art-de1cdf5815b542458a97f3955ace0f6c |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-04-12T18:55:12Z |
| publishDate | 2018-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-de1cdf5815b542458a97f3955ace0f6c2022-12-22T03:20:22ZengTaylor & Francis GroupVirulence2150-55942150-56082018-12-01911176119410.1080/21505594.2018.14967761496776Autophagy enhances the replication of Peste des petits ruminants virus and inhibits caspase-dependent apoptosis in vitroBo Yang0Qinghong Xue1Xuefeng Qi2Xueping Wang3Peilong Jia4Shuying Chen5Ting Wang6Tianxia Xue7Jingyu Wang8Northwest A&F UniversityChina Institute of Veterinary Drug ControlNorthwest A&F UniversityNorthwest A&F UniversityNorthwest A&F UniversityNorthwest A&F UniversityNorthwest A&F UniversityNorthwest A&F UniversityNorthwest A&F UniversityPeste des petits ruminants (PPR) is an acute and highly contagious disease in small ruminants that causes significant economic losses in developing countries. An increasing number of studies have demonstrated that both autophagy and apoptosis are important cellular mechanisms for maintaining homeostasis, and they participate in the host response to pathogens. However, the crosstalk between apoptosis and autophagy in host cells during PPRV infection has not been clarified. In this study, autophagy was induced upon virus infection in caprine endometrial epithelial cells (EECs), as determined by the appearance of double- and single-membrane autophagy-like vesicles, LC3-I/LC3-II conversion, and p62 degradation. We also found that PPRV infection triggered a complete autophagic response, most likely mediated by the non-structural protein C and nucleoprotein N. Moreover, our results suggest that autophagy not only promotes the replication of PPRV in EECs but also provides a potential mechanism for inhibiting PPRV-induced apoptosis. Inhibiting autophagosome formation by wortmannin and knocking down the essential autophagic proteins Beclin-1 and ATG7 induces caspase-dependent apoptosis in EECs in PPRV infection. However, inhibiting autophagosome and lysosome fusion by NH4Cl and chloroquine did not increase the number of apoptotic cells. Collectively, these data are the first to indicate that PPRV-induced autophagy inhibits caspase-dependent apoptosis and thus contributes to the enhancement of viral replication and maturity in host cells.http://dx.doi.org/10.1080/21505594.2018.1496776Peste des petits ruminants virus (PPRV)autophagyapoptosisreplicationcaprine endometrial epithelial cells (EECs) |
| spellingShingle | Bo Yang Qinghong Xue Xuefeng Qi Xueping Wang Peilong Jia Shuying Chen Ting Wang Tianxia Xue Jingyu Wang Autophagy enhances the replication of Peste des petits ruminants virus and inhibits caspase-dependent apoptosis in vitro Virulence Peste des petits ruminants virus (PPRV) autophagy apoptosis replication caprine endometrial epithelial cells (EECs) |
| title | Autophagy enhances the replication of Peste des petits ruminants virus and inhibits caspase-dependent apoptosis in vitro |
| title_full | Autophagy enhances the replication of Peste des petits ruminants virus and inhibits caspase-dependent apoptosis in vitro |
| title_fullStr | Autophagy enhances the replication of Peste des petits ruminants virus and inhibits caspase-dependent apoptosis in vitro |
| title_full_unstemmed | Autophagy enhances the replication of Peste des petits ruminants virus and inhibits caspase-dependent apoptosis in vitro |
| title_short | Autophagy enhances the replication of Peste des petits ruminants virus and inhibits caspase-dependent apoptosis in vitro |
| title_sort | autophagy enhances the replication of peste des petits ruminants virus and inhibits caspase dependent apoptosis in vitro |
| topic | Peste des petits ruminants virus (PPRV) autophagy apoptosis replication caprine endometrial epithelial cells (EECs) |
| url | http://dx.doi.org/10.1080/21505594.2018.1496776 |
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