Quantitative RT-PCR analysis and immunohistochemical localization of HSP70 in sea bass Dicentrarchus labraxexposed to transport stress
In aquaculture, fish are exposed to stressful conditions, which cause an increased synthesis of heat shock proteins (HSPs) at the cellular level. In this work we considered the expression of the constitutive and inducible forms of HSP70 as an indicator of stress caused by transport, during developme...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2009-08-01
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| Series: | European Journal of Histochemistry |
| Online Access: | http://www.ejh.it/index.php/ejh/article/view/1134 |
| _version_ | 1818258311593590784 |
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| author | C Poltronieri L Maccatrozzo C Simontacchi D Bertotto B Funkenstein M Patruno G Radaelli |
| author_facet | C Poltronieri L Maccatrozzo C Simontacchi D Bertotto B Funkenstein M Patruno G Radaelli |
| author_sort | C Poltronieri |
| collection | DOAJ |
| description | In aquaculture, fish are exposed to stressful conditions, which cause an increased synthesis of heat shock proteins (HSPs) at the cellular level. In this work we considered the expression of the constitutive and inducible forms of HSP70 as an indicator of stress caused by transport, during development of the sea bass (Dicentrarchus labrax), a teleost fish of high value for aquaculture. Qualitative RT-PCR analysis revealed expression of inducible HSP70 gene in larvae and fry (25, 40 and 80 days) as well as in adult tissues (liver, brain, muscle, gills, kidney, gonads, heart, spleen and skin) of both control and stressed animals. Expression of inducible HSP70 mRNA examined in different adult tissues by Real- Time PCR, was significantly higher in skin and skeletal muscle of stressed animals than in controls. Immunolocalization of inducible and constitutive forms of heat shock protein 70 (HSP70 and HSC70), reported here for the first time, demonstrated an ubiquitous distribution of HSC70 protein in several tissues of both stressed and control animals (at all stages), while inducible HSP70 protein was found only in skeletal muscle of stressed animals. In all stressed animals, regardless of their developmental stage, cortisol levels were higher than in control animals. |
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| id | doaj.art-de20e18f450d4cb59a921b2adb3cab8d |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-12T17:57:32Z |
| publishDate | 2009-08-01 |
| publisher | PAGEPress Publications |
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| series | European Journal of Histochemistry |
| spelling | doaj.art-de20e18f450d4cb59a921b2adb3cab8d2022-12-22T00:16:42ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062009-08-0151212513610.4081/1134768Quantitative RT-PCR analysis and immunohistochemical localization of HSP70 in sea bass Dicentrarchus labraxexposed to transport stressC PoltronieriL MaccatrozzoC SimontacchiD BertottoB FunkensteinM PatrunoG RadaelliIn aquaculture, fish are exposed to stressful conditions, which cause an increased synthesis of heat shock proteins (HSPs) at the cellular level. In this work we considered the expression of the constitutive and inducible forms of HSP70 as an indicator of stress caused by transport, during development of the sea bass (Dicentrarchus labrax), a teleost fish of high value for aquaculture. Qualitative RT-PCR analysis revealed expression of inducible HSP70 gene in larvae and fry (25, 40 and 80 days) as well as in adult tissues (liver, brain, muscle, gills, kidney, gonads, heart, spleen and skin) of both control and stressed animals. Expression of inducible HSP70 mRNA examined in different adult tissues by Real- Time PCR, was significantly higher in skin and skeletal muscle of stressed animals than in controls. Immunolocalization of inducible and constitutive forms of heat shock protein 70 (HSP70 and HSC70), reported here for the first time, demonstrated an ubiquitous distribution of HSC70 protein in several tissues of both stressed and control animals (at all stages), while inducible HSP70 protein was found only in skeletal muscle of stressed animals. In all stressed animals, regardless of their developmental stage, cortisol levels were higher than in control animals.http://www.ejh.it/index.php/ejh/article/view/1134 |
| spellingShingle | C Poltronieri L Maccatrozzo C Simontacchi D Bertotto B Funkenstein M Patruno G Radaelli Quantitative RT-PCR analysis and immunohistochemical localization of HSP70 in sea bass Dicentrarchus labraxexposed to transport stress European Journal of Histochemistry |
| title | Quantitative RT-PCR analysis and immunohistochemical localization of HSP70 in sea bass Dicentrarchus labraxexposed to transport stress |
| title_full | Quantitative RT-PCR analysis and immunohistochemical localization of HSP70 in sea bass Dicentrarchus labraxexposed to transport stress |
| title_fullStr | Quantitative RT-PCR analysis and immunohistochemical localization of HSP70 in sea bass Dicentrarchus labraxexposed to transport stress |
| title_full_unstemmed | Quantitative RT-PCR analysis and immunohistochemical localization of HSP70 in sea bass Dicentrarchus labraxexposed to transport stress |
| title_short | Quantitative RT-PCR analysis and immunohistochemical localization of HSP70 in sea bass Dicentrarchus labraxexposed to transport stress |
| title_sort | quantitative rt pcr analysis and immunohistochemical localization of hsp70 in sea bass dicentrarchus labraxexposed to transport stress |
| url | http://www.ejh.it/index.php/ejh/article/view/1134 |
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