Bioconversion of Lactose into Glucose–Galactose Syrup by Two-Stage Enzymatic Hydrolysis
Fermentation technology enables the better use of resources and the conversion of dairy waste into valuable food products. The aim of this study is to evaluate the conversion rate of glucose into fructose by immobilised glucose isomerase (GI) in sweet and acid whey permeates for glucose–galactose sy...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/3/400 |
| _version_ | 1797487806535172096 |
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| author | Kristine Majore Inga Ciprovica |
| author_facet | Kristine Majore Inga Ciprovica |
| author_sort | Kristine Majore |
| collection | DOAJ |
| description | Fermentation technology enables the better use of resources and the conversion of dairy waste into valuable food products. The aim of this study is to evaluate the conversion rate of glucose into fructose by immobilised glucose isomerase (GI) in sweet and acid whey permeates for glucose–galactose syrup production. The experiments demonstrated that the highest concentration of glucose and galacto-oligosaccharides (GOSs) in sweet and acid whey permeates was reached by GODO-YNL2 β-galactosidase, 32 ± 2% and 28 ± 1%, respectively. After glucose isomerisation, the highest fructose yield was 23 ± 0.3% and 13 ± 0.4% in sweet and acid whey permeates, where Ha-Lactase 5200 β-galactosidase was used for lactose hydrolysis in sweet and acid whey permeates. Finally, the results of this study highlight the potential for two-stage enzymatic hydrolysis to increase the sweetness of glucose–galactose syrup made from sweet and acid whey permeates. |
| first_indexed | 2024-03-09T23:53:02Z |
| format | Article |
| id | doaj.art-de217464a32d471fb6069edd88b655dd |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T23:53:02Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-de217464a32d471fb6069edd88b655dd2023-11-23T16:30:20ZengMDPI AGFoods2304-81582022-01-0111340010.3390/foods11030400Bioconversion of Lactose into Glucose–Galactose Syrup by Two-Stage Enzymatic HydrolysisKristine Majore0Inga Ciprovica1Faculty of Food Technology, Latvia University of Life Sciences and Technologies, Rigas iela 22, LV-3004 Jelgava, LatviaFaculty of Food Technology, Latvia University of Life Sciences and Technologies, Rigas iela 22, LV-3004 Jelgava, LatviaFermentation technology enables the better use of resources and the conversion of dairy waste into valuable food products. The aim of this study is to evaluate the conversion rate of glucose into fructose by immobilised glucose isomerase (GI) in sweet and acid whey permeates for glucose–galactose syrup production. The experiments demonstrated that the highest concentration of glucose and galacto-oligosaccharides (GOSs) in sweet and acid whey permeates was reached by GODO-YNL2 β-galactosidase, 32 ± 2% and 28 ± 1%, respectively. After glucose isomerisation, the highest fructose yield was 23 ± 0.3% and 13 ± 0.4% in sweet and acid whey permeates, where Ha-Lactase 5200 β-galactosidase was used for lactose hydrolysis in sweet and acid whey permeates. Finally, the results of this study highlight the potential for two-stage enzymatic hydrolysis to increase the sweetness of glucose–galactose syrup made from sweet and acid whey permeates.https://www.mdpi.com/2304-8158/11/3/400β-galactosidasepermeateglucose isomerasesyrupGOS |
| spellingShingle | Kristine Majore Inga Ciprovica Bioconversion of Lactose into Glucose–Galactose Syrup by Two-Stage Enzymatic Hydrolysis Foods β-galactosidase permeate glucose isomerase syrup GOS |
| title | Bioconversion of Lactose into Glucose–Galactose Syrup by Two-Stage Enzymatic Hydrolysis |
| title_full | Bioconversion of Lactose into Glucose–Galactose Syrup by Two-Stage Enzymatic Hydrolysis |
| title_fullStr | Bioconversion of Lactose into Glucose–Galactose Syrup by Two-Stage Enzymatic Hydrolysis |
| title_full_unstemmed | Bioconversion of Lactose into Glucose–Galactose Syrup by Two-Stage Enzymatic Hydrolysis |
| title_short | Bioconversion of Lactose into Glucose–Galactose Syrup by Two-Stage Enzymatic Hydrolysis |
| title_sort | bioconversion of lactose into glucose galactose syrup by two stage enzymatic hydrolysis |
| topic | β-galactosidase permeate glucose isomerase syrup GOS |
| url | https://www.mdpi.com/2304-8158/11/3/400 |
| work_keys_str_mv | AT kristinemajore bioconversionoflactoseintoglucosegalactosesyrupbytwostageenzymatichydrolysis AT ingaciprovica bioconversionoflactoseintoglucosegalactosesyrupbytwostageenzymatichydrolysis |