A Single Seed Protein Extraction Protocol for Characterizing Brassica Seed Storage Proteins

A Single Seed Protein Extraction Protocol for Characterizing Brassica Seed Storage Proteins

Rapeseed oil-extracted expeller cake mostly contains protein. Various approaches have been used to isolate, detect and measure proteins in rapeseeds, with a particular focus on seed storage proteins (SSPs). To maximize the protein yield and minimize hazardous chemical use, isolation costs and the lo...

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Bibliographic Details
Main Authors: Mahmudur Rahman, Lei Liu, Bronwyn J. Barkla
Format: Article
Language:English
Published: MDPI AG 2021-01-01
Series:Agronomy
Subjects:
protein extraction
seed proteins
<i>Brassica rapa</i>
2S albumin like napins
11/12S globulin like cruciferins
1D SDS-PAGE
Online Access:https://www.mdpi.com/2073-4395/11/1/107

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