In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study
Aim: An approach for three-dimensional (3D) bone tissue generation from bone marrow mesenchymal adult stem cells (BMSC-AS) was investigated. Methods: The BMSC-AS cells were induced to differentiate into osteogenic precursors, capable of proliferating, and subsequently differentiating into bone-formi...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Campinas
2015-12-01
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| Series: | Brazilian Journal of Oral Sciences |
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| Online Access: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642090 |
| _version_ | 1818649414509527040 |
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| author | K. A. AL-Salihi |
| author_facet | K. A. AL-Salihi |
| author_sort | K. A. AL-Salihi |
| collection | DOAJ |
| description | Aim: An approach for three-dimensional (3D) bone tissue generation from bone marrow mesenchymal adult stem cells (BMSC-AS) was investigated. Methods: The BMSC-AS cells were induced to differentiate into osteogenic precursors, capable of proliferating, and subsequently differentiating into bone-forming cells. The differentiated cells were seeded on the surface of coral discs with a mean diameter 10 (±2) mm and a mean thickness 1 (±0.5) mm. The seeded scaffolds were characterized using von Kossa and Alizarin Red staining, electron and confocal microscopy and RT-PCR analysis. Results: The results demonstrated that BMSC-AS derived bone-forming cells attached to and colonized into coral scaffolds. Furthermore, these cells produced bone nodules when grown for 3-4 weeks in mineralization medium containing ascorbic acid and beta-glycerophosphate both in tissue culture plates and in scaffolds. The differentiated cells also expressed osteospecific markers when grown both in the culture plates and in 3D scaffolds. Osteogenic cells expressed alkaline phosphatase, osteocalcin, and osteopontin, but not a BMSC-AS cell-specific marker, oct-4. Conclusion: These findings suggest that Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) with BMSC-AS cells can be used for in vitro tissue engineering to cultivation of graftable skeletal structures. |
| first_indexed | 2024-12-17T01:33:57Z |
| format | Article |
| id | doaj.art-de23fa97f1994c888891bc6435219234 |
| institution | Directory Open Access Journal |
| issn | 1677-3225 |
| language | English |
| last_indexed | 2024-12-17T01:33:57Z |
| publishDate | 2015-12-01 |
| publisher | Universidade Estadual de Campinas |
| record_format | Article |
| series | Brazilian Journal of Oral Sciences |
| spelling | doaj.art-de23fa97f1994c888891bc64352192342022-12-21T22:08:30ZengUniversidade Estadual de CampinasBrazilian Journal of Oral Sciences1677-32252015-12-018410.20396/bjos.v8i4.8642090In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary studyK. A. AL-Salihi0University Sains MalaysiaAim: An approach for three-dimensional (3D) bone tissue generation from bone marrow mesenchymal adult stem cells (BMSC-AS) was investigated. Methods: The BMSC-AS cells were induced to differentiate into osteogenic precursors, capable of proliferating, and subsequently differentiating into bone-forming cells. The differentiated cells were seeded on the surface of coral discs with a mean diameter 10 (±2) mm and a mean thickness 1 (±0.5) mm. The seeded scaffolds were characterized using von Kossa and Alizarin Red staining, electron and confocal microscopy and RT-PCR analysis. Results: The results demonstrated that BMSC-AS derived bone-forming cells attached to and colonized into coral scaffolds. Furthermore, these cells produced bone nodules when grown for 3-4 weeks in mineralization medium containing ascorbic acid and beta-glycerophosphate both in tissue culture plates and in scaffolds. The differentiated cells also expressed osteospecific markers when grown both in the culture plates and in 3D scaffolds. Osteogenic cells expressed alkaline phosphatase, osteocalcin, and osteopontin, but not a BMSC-AS cell-specific marker, oct-4. Conclusion: These findings suggest that Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) with BMSC-AS cells can be used for in vitro tissue engineering to cultivation of graftable skeletal structures.https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642090In vitroCoral reefCORAGRAF3 D scaffoldsBone tissue engineering |
| spellingShingle | K. A. AL-Salihi In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study Brazilian Journal of Oral Sciences In vitro Coral reef CORAGRAF 3 D scaffolds Bone tissue engineering |
| title | In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study |
| title_full | In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study |
| title_fullStr | In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study |
| title_full_unstemmed | In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study |
| title_short | In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study |
| title_sort | in vitro evaluation of malaysian natural coral porites bone graft substitutes coragraf for bone tissue engineering a preliminary study |
| topic | In vitro Coral reef CORAGRAF 3 D scaffolds Bone tissue engineering |
| url | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642090 |
| work_keys_str_mv | AT kaalsalihi invitroevaluationofmalaysiannaturalcoralporitesbonegraftsubstitutescoragrafforbonetissueengineeringapreliminarystudy |