Comparative Analysis of Microbial Consortiums and Nanoparticles for Rehabilitating Petroleum Waste Contaminated Soils
Nano-bioremediation application is an ecologically and environmentally friendly technique to overcome the catastrophic situation in soil because of petroleum waste contamination. We evaluated the efficiency of oil-degrading bacterial consortium and silver nanoparticles (AgNPs) with or without fertil...
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MDPI AG
2022-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/6/1945 |
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| author | Shehla Sattar Samina Siddiqui Asim Shahzad Asghari Bano Muhammad Naeem Rahib Hussain Naeem Khan Basit Latief Jan Humaira Yasmin |
| author_facet | Shehla Sattar Samina Siddiqui Asim Shahzad Asghari Bano Muhammad Naeem Rahib Hussain Naeem Khan Basit Latief Jan Humaira Yasmin |
| author_sort | Shehla Sattar |
| collection | DOAJ |
| description | Nano-bioremediation application is an ecologically and environmentally friendly technique to overcome the catastrophic situation in soil because of petroleum waste contamination. We evaluated the efficiency of oil-degrading bacterial consortium and silver nanoparticles (AgNPs) with or without fertilizer to remediate soils collected from petroleum waste contaminated oil fields. Physicochemical characteristics of control soil and petroleum contaminated soils were assessed. Four oil-degrading strains, namely <i>Bacillus pumilus</i> (KY010576), <i>Exiguobacteriaum aurantiacum</i> (KY010578), <i>Lysinibacillus fusiformis</i> (KY010586), and <i>Pseudomonas putida</i> (KX580766), were selected based on their in vitrohydrocarbon-degrading efficiency. In a lab experiment, contaminated soils were treated alone and with combined amendments of the bacterial consortium, AgNPs, and fertilizers (ammonium nitrate and diammonium phosphate). We detected the degradation rate of total petroleum hydrocarbons (TPHs) of the soil samples with GC-FID at different intervals of the incubation period (0, 5, 20, 60, 240 days). The bacterial population (CFU/g) was also monitored during the entire period of incubation. The results showed that 70% more TPH was degraded with a consortium with their sole application in 20 days of incubation. There was a positive correlation between TPH degradation and the 100-fold increase in bacterial population in contaminated soils. This study revealed that bacterial consortiums alone showed the maximum increase in the degradation of TPHs at 20 days. The application of nanoparticles and fertilizer has non-significant effects on the consortium degradation potential. Moreover, fertilizer alone or in combination with AgNPs and consortium slows the rate of degradation of TPHs over a short period. Still, it subsequently accelerates the rate of degradation of TPHs, and a negligible amount remains at the end of the incubation period. |
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| id | doaj.art-de247447bd73401aaee5b8a8ee73d5be |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T13:10:05Z |
| publishDate | 2022-03-01 |
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| series | Molecules |
| spelling | doaj.art-de247447bd73401aaee5b8a8ee73d5be2023-11-30T21:43:38ZengMDPI AGMolecules1420-30492022-03-01276194510.3390/molecules27061945Comparative Analysis of Microbial Consortiums and Nanoparticles for Rehabilitating Petroleum Waste Contaminated SoilsShehla Sattar0Samina Siddiqui1Asim Shahzad2Asghari Bano3Muhammad Naeem4Rahib Hussain5Naeem Khan6Basit Latief Jan7Humaira Yasmin8National Centre of Excellence in Geology, University of Peshawar, Peshawar 25130, PakistanNational Centre of Excellence in Geology, University of Peshawar, Peshawar 25130, PakistanDepartment of Botany, Mohi-ud-Din Islamic University, Nerian Sharif 12080, PakistanDepartment of Bio-Sciences, Quaid Avenue University of Wah, Wah 47000, PakistanDepartment of Biotechnology, Mohi-ud-Din Islamic University, Nerian Sharif 12080, PakistanNational Centre of Excellence in Geology, University of Peshawar, Peshawar 25130, PakistanDepartment of Agronomy, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USADepartment of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Biosciences, COMSATS University Islamabad (CUI), Islamabad 45550, PakistanNano-bioremediation application is an ecologically and environmentally friendly technique to overcome the catastrophic situation in soil because of petroleum waste contamination. We evaluated the efficiency of oil-degrading bacterial consortium and silver nanoparticles (AgNPs) with or without fertilizer to remediate soils collected from petroleum waste contaminated oil fields. Physicochemical characteristics of control soil and petroleum contaminated soils were assessed. Four oil-degrading strains, namely <i>Bacillus pumilus</i> (KY010576), <i>Exiguobacteriaum aurantiacum</i> (KY010578), <i>Lysinibacillus fusiformis</i> (KY010586), and <i>Pseudomonas putida</i> (KX580766), were selected based on their in vitrohydrocarbon-degrading efficiency. In a lab experiment, contaminated soils were treated alone and with combined amendments of the bacterial consortium, AgNPs, and fertilizers (ammonium nitrate and diammonium phosphate). We detected the degradation rate of total petroleum hydrocarbons (TPHs) of the soil samples with GC-FID at different intervals of the incubation period (0, 5, 20, 60, 240 days). The bacterial population (CFU/g) was also monitored during the entire period of incubation. The results showed that 70% more TPH was degraded with a consortium with their sole application in 20 days of incubation. There was a positive correlation between TPH degradation and the 100-fold increase in bacterial population in contaminated soils. This study revealed that bacterial consortiums alone showed the maximum increase in the degradation of TPHs at 20 days. The application of nanoparticles and fertilizer has non-significant effects on the consortium degradation potential. Moreover, fertilizer alone or in combination with AgNPs and consortium slows the rate of degradation of TPHs over a short period. Still, it subsequently accelerates the rate of degradation of TPHs, and a negligible amount remains at the end of the incubation period.https://www.mdpi.com/1420-3049/27/6/1945nano-bioremediationconsortiumnanoparticlespetroleum degradationtotal petroleum hydrocarbonschemical fertilizer |
| spellingShingle | Shehla Sattar Samina Siddiqui Asim Shahzad Asghari Bano Muhammad Naeem Rahib Hussain Naeem Khan Basit Latief Jan Humaira Yasmin Comparative Analysis of Microbial Consortiums and Nanoparticles for Rehabilitating Petroleum Waste Contaminated Soils Molecules nano-bioremediation consortium nanoparticles petroleum degradation total petroleum hydrocarbons chemical fertilizer |
| title | Comparative Analysis of Microbial Consortiums and Nanoparticles for Rehabilitating Petroleum Waste Contaminated Soils |
| title_full | Comparative Analysis of Microbial Consortiums and Nanoparticles for Rehabilitating Petroleum Waste Contaminated Soils |
| title_fullStr | Comparative Analysis of Microbial Consortiums and Nanoparticles for Rehabilitating Petroleum Waste Contaminated Soils |
| title_full_unstemmed | Comparative Analysis of Microbial Consortiums and Nanoparticles for Rehabilitating Petroleum Waste Contaminated Soils |
| title_short | Comparative Analysis of Microbial Consortiums and Nanoparticles for Rehabilitating Petroleum Waste Contaminated Soils |
| title_sort | comparative analysis of microbial consortiums and nanoparticles for rehabilitating petroleum waste contaminated soils |
| topic | nano-bioremediation consortium nanoparticles petroleum degradation total petroleum hydrocarbons chemical fertilizer |
| url | https://www.mdpi.com/1420-3049/27/6/1945 |
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