Relation between the Presence of Mouse Mammary Tumor Virus-Like Sequences in Breast Tissues and Human Breast Cancer in Iranian Patients
Background: Mouse mammary tumor virus (MMTV), a milk-transmitted retrovirus, is considered to be involved in the development of breast tumors in mice. Although several previous studies have identified MMTV-like sequences in human breast cancer, the presence of human endogenous retrovirus (HERs) make...
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| Format: | Article |
| Language: | fas |
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Isfahan University of Medical Sciences
2013-05-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/2603 |
| _version_ | 1797716283633958912 |
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| author | Seyed Shervin Shariatpanahi Mohammad Soleimani Darjagh Rasoul Salehi |
| author_facet | Seyed Shervin Shariatpanahi Mohammad Soleimani Darjagh Rasoul Salehi |
| author_sort | Seyed Shervin Shariatpanahi |
| collection | DOAJ |
| description | Background: Mouse mammary tumor virus (MMTV), a milk-transmitted retrovirus, is considered to be involved in the development of breast tumors in mice. Although several previous studies have identified MMTV-like sequences in human breast cancer, the presence of human endogenous retrovirus (HERs) makes these results controversial, i.e. the role of MMTV in breast cancer has never been definitely proven. In addition, various results have been reported in this regard from different countries. We selected MMTV envelope (Env) gene sequences to eliminate the effects of HERs.
Methods: In this case-control study, DNA was extracted from 59 paraffin-embedded malignant tumor tissues and 59 normal control samples. Nested polymerase chain reaction (PCR) was then used to detect MMTV Env-like sequences.
Findings: MMTV genome was detected in 19 malignant breast samples (32%) and three normal control samples (5%) (P < 0.001).
Conclusion: Based on our findings, MMTV can play a major role in the development of breast cancer in Iranian women. According to PCR results, the obtained sequences had 99% homology with BR6 strain of MMTV and 100% homology with GR and C3H strains. |
| first_indexed | 2024-03-12T08:20:10Z |
| format | Article |
| id | doaj.art-de256cfaf994474e989867a9eed63912 |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T08:20:10Z |
| publishDate | 2013-05-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-de256cfaf994474e989867a9eed639122023-09-02T18:36:25ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2013-05-01312272082171290Relation between the Presence of Mouse Mammary Tumor Virus-Like Sequences in Breast Tissues and Human Breast Cancer in Iranian PatientsSeyed Shervin Shariatpanahi0Mohammad Soleimani Darjagh1Rasoul Salehi2Department of Microbiology, Islamic Azad University, Qom Branch, Qom, IranAssistant Professor, Department of Microbiology, Islamic Azad University, Qom Branch, Qom, IranAssociate Professor, Pediatric Diseases Research Center AND Department of Anatomy and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground: Mouse mammary tumor virus (MMTV), a milk-transmitted retrovirus, is considered to be involved in the development of breast tumors in mice. Although several previous studies have identified MMTV-like sequences in human breast cancer, the presence of human endogenous retrovirus (HERs) makes these results controversial, i.e. the role of MMTV in breast cancer has never been definitely proven. In addition, various results have been reported in this regard from different countries. We selected MMTV envelope (Env) gene sequences to eliminate the effects of HERs. Methods: In this case-control study, DNA was extracted from 59 paraffin-embedded malignant tumor tissues and 59 normal control samples. Nested polymerase chain reaction (PCR) was then used to detect MMTV Env-like sequences. Findings: MMTV genome was detected in 19 malignant breast samples (32%) and three normal control samples (5%) (P < 0.001). Conclusion: Based on our findings, MMTV can play a major role in the development of breast cancer in Iranian women. According to PCR results, the obtained sequences had 99% homology with BR6 strain of MMTV and 100% homology with GR and C3H strains.http://jims.mui.ac.ir/index.php/jims/article/view/2603Mouse mammary tumor virusBreast cancerMalignant tumorsRisk factorsNested polymerase chain reaction |
| spellingShingle | Seyed Shervin Shariatpanahi Mohammad Soleimani Darjagh Rasoul Salehi Relation between the Presence of Mouse Mammary Tumor Virus-Like Sequences in Breast Tissues and Human Breast Cancer in Iranian Patients مجله دانشکده پزشکی اصفهان Mouse mammary tumor virus Breast cancer Malignant tumors Risk factors Nested polymerase chain reaction |
| title | Relation between the Presence of Mouse Mammary Tumor Virus-Like Sequences in Breast Tissues and Human Breast Cancer in Iranian Patients |
| title_full | Relation between the Presence of Mouse Mammary Tumor Virus-Like Sequences in Breast Tissues and Human Breast Cancer in Iranian Patients |
| title_fullStr | Relation between the Presence of Mouse Mammary Tumor Virus-Like Sequences in Breast Tissues and Human Breast Cancer in Iranian Patients |
| title_full_unstemmed | Relation between the Presence of Mouse Mammary Tumor Virus-Like Sequences in Breast Tissues and Human Breast Cancer in Iranian Patients |
| title_short | Relation between the Presence of Mouse Mammary Tumor Virus-Like Sequences in Breast Tissues and Human Breast Cancer in Iranian Patients |
| title_sort | relation between the presence of mouse mammary tumor virus like sequences in breast tissues and human breast cancer in iranian patients |
| topic | Mouse mammary tumor virus Breast cancer Malignant tumors Risk factors Nested polymerase chain reaction |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/2603 |
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