Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method
Interleukin-6 (IL-6) is a pro/anti-inflammatory cytokine, the quantitative detection of which has been extensively considered for diagnosis of inflammatory associated diseases. However, there has not yet been a reliable, low-cost, and user-friendly platform developed for point-of-care (POC) detectio...
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| Format: | Article |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.778269/full |
| _version_ | 1818742763937595392 |
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| author | Mohammad Rahbar Yuling Wu J. Anand Subramony Guozhen Liu Guozhen Liu |
| author_facet | Mohammad Rahbar Yuling Wu J. Anand Subramony Guozhen Liu Guozhen Liu |
| author_sort | Mohammad Rahbar |
| collection | DOAJ |
| description | Interleukin-6 (IL-6) is a pro/anti-inflammatory cytokine, the quantitative detection of which has been extensively considered for diagnosis of inflammatory associated diseases. However, there has not yet been a reliable, low-cost, and user-friendly platform developed for point-of-care (POC) detection of IL-6, which will eliminate the conventional costly, time-consuming, and complex assays. In this work, we developed a lateral flow assay for colorimetric detection of IL-6, using anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs) as the detection probes. Silver amplification technique was incorporated with the newly developed assay in order to enhance the obtained colorimetric signals, allowing sensitive detection of IL-6 in human serum in the desired physiological ranges (i.e., 5–1000 pg/mL). A limit of detection of 5 pg/mL could be achieved for IL-6 detection in serum with the amplification step which was not achievable in the standard assay. The corresponding specificity and reproducibility tests were all preformed to confirm the reliability of this assay for quantitative measurement of IL-6 in a POC manner. |
| first_indexed | 2024-12-18T02:17:42Z |
| format | Article |
| id | doaj.art-de25c03fa2fc4e6a904e4c80afd7cd3b |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-18T02:17:42Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-de25c03fa2fc4e6a904e4c80afd7cd3b2022-12-21T21:24:20ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-11-01910.3389/fbioe.2021.778269778269Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification MethodMohammad Rahbar0Yuling Wu1J. Anand Subramony2Guozhen Liu3Guozhen Liu4Graduate School of Biomedical Engineering, The University of New South Wales, Kensington, NSW, AustraliaIntegrated Bioanalysis, Clinical Pharmacology and Quantitative Pharmacology, BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, MD, United StatesBiologics Engineering R&D, AstraZeneca, Gaithersburg, MD, United StatesGraduate School of Biomedical Engineering, The University of New South Wales, Kensington, NSW, AustraliaSchool of Life and Health Sciences, The Chinese University of Hong Kong, Shenzhen, ChinaInterleukin-6 (IL-6) is a pro/anti-inflammatory cytokine, the quantitative detection of which has been extensively considered for diagnosis of inflammatory associated diseases. However, there has not yet been a reliable, low-cost, and user-friendly platform developed for point-of-care (POC) detection of IL-6, which will eliminate the conventional costly, time-consuming, and complex assays. In this work, we developed a lateral flow assay for colorimetric detection of IL-6, using anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs) as the detection probes. Silver amplification technique was incorporated with the newly developed assay in order to enhance the obtained colorimetric signals, allowing sensitive detection of IL-6 in human serum in the desired physiological ranges (i.e., 5–1000 pg/mL). A limit of detection of 5 pg/mL could be achieved for IL-6 detection in serum with the amplification step which was not achievable in the standard assay. The corresponding specificity and reproducibility tests were all preformed to confirm the reliability of this assay for quantitative measurement of IL-6 in a POC manner.https://www.frontiersin.org/articles/10.3389/fbioe.2021.778269/fullpoint-of-care diagnosticslateral flow immunoassayinterleukin-6silver enhancement reagentpaper test stripscolorimetric detection |
| spellingShingle | Mohammad Rahbar Yuling Wu J. Anand Subramony Guozhen Liu Guozhen Liu Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method Frontiers in Bioengineering and Biotechnology point-of-care diagnostics lateral flow immunoassay interleukin-6 silver enhancement reagent paper test strips colorimetric detection |
| title | Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method |
| title_full | Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method |
| title_fullStr | Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method |
| title_full_unstemmed | Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method |
| title_short | Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method |
| title_sort | sensitive colorimetric detection of interleukin 6 via lateral flow assay incorporated silver amplification method |
| topic | point-of-care diagnostics lateral flow immunoassay interleukin-6 silver enhancement reagent paper test strips colorimetric detection |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.778269/full |
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