Taurine protection of PC12 cells against endoplasmic reticulum stress induced by oxidative stress
<p>Abstract</p> <p>Background</p> <p>Taurine is a free amino acid present in high concentrations in a variety of organs of mammalians. As an antioxidant, taurine has been found to protect cells against oxidative stress, but the underlying mechanism is still unclear.<...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-08-01
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| Series: | Journal of Biomedical Science |
| _version_ | 1811276606006624256 |
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| author | Pan Chunliu Giraldo Grace S Prentice Howard Wu Jang-Yen |
| author_facet | Pan Chunliu Giraldo Grace S Prentice Howard Wu Jang-Yen |
| author_sort | Pan Chunliu |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Taurine is a free amino acid present in high concentrations in a variety of organs of mammalians. As an antioxidant, taurine has been found to protect cells against oxidative stress, but the underlying mechanism is still unclear.</p> <p>Methods</p> <p>In this report, we present evidence to support the conclusion that taurine exerts a protective function against endoplasmic reticulum (ER) stress induced by H<sub>2</sub>O<sub>2</sub> in PC 12 cells. Oxidative stress was introduced by exposure of PC 12 cells to 250 uM H<sub>2</sub>O<sub>2</sub> for 4 hours.</p> <p>Results</p> <p>It was found that the cell viability of PC 12 cells decreased with an increase of H<sub>2</sub>O<sub>2</sub> concentration ranging from approximately 76% cell viability at 100 uM H<sub>2</sub>O<sub>2</sub> down to 18% at 500 uM H<sub>2</sub>O<sub>2</sub>. At 250 uM H<sub>2</sub>O<sub>2</sub>, cell viability was restored to 80% by taurine at 25 mM. Furthermore, H<sub>2</sub>O<sub>2</sub> treatment also caused a marked reduction in the expression of Bcl-2 while no significant change of Bax was observed. Treatment with taurine restored the reduced expression of Bcl-2 close to the control level without any obvious effect on Bax. Furthermore, taurine was also found to suppress up-regulation of GRP78, GADD153/CHOP and Bim induced by H<sub>2</sub>O<sub>2</sub>, suggesting that taurine may also exert a protective function against oxidative stress by reducing the ER stress.</p> <p>Conclusion</p> <p>In summary, taurine was shown to protect PC12 cells against oxidative stress induced by H<sub>2</sub>O<sub>2</sub>. ER stress was induced by oxidative stress and can be suppressed by taurine.</p> |
| first_indexed | 2024-04-13T00:00:45Z |
| format | Article |
| id | doaj.art-de25dbe28aee417a842dc3f84c3ed1a2 |
| institution | Directory Open Access Journal |
| issn | 1021-7770 1423-0127 |
| language | English |
| last_indexed | 2024-04-13T00:00:45Z |
| publishDate | 2010-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biomedical Science |
| spelling | doaj.art-de25dbe28aee417a842dc3f84c3ed1a22022-12-22T03:11:22ZengBMCJournal of Biomedical Science1021-77701423-01272010-08-0117Suppl 1S1710.1186/1423-0127-17-S1-S17Taurine protection of PC12 cells against endoplasmic reticulum stress induced by oxidative stressPan ChunliuGiraldo Grace SPrentice HowardWu Jang-Yen<p>Abstract</p> <p>Background</p> <p>Taurine is a free amino acid present in high concentrations in a variety of organs of mammalians. As an antioxidant, taurine has been found to protect cells against oxidative stress, but the underlying mechanism is still unclear.</p> <p>Methods</p> <p>In this report, we present evidence to support the conclusion that taurine exerts a protective function against endoplasmic reticulum (ER) stress induced by H<sub>2</sub>O<sub>2</sub> in PC 12 cells. Oxidative stress was introduced by exposure of PC 12 cells to 250 uM H<sub>2</sub>O<sub>2</sub> for 4 hours.</p> <p>Results</p> <p>It was found that the cell viability of PC 12 cells decreased with an increase of H<sub>2</sub>O<sub>2</sub> concentration ranging from approximately 76% cell viability at 100 uM H<sub>2</sub>O<sub>2</sub> down to 18% at 500 uM H<sub>2</sub>O<sub>2</sub>. At 250 uM H<sub>2</sub>O<sub>2</sub>, cell viability was restored to 80% by taurine at 25 mM. Furthermore, H<sub>2</sub>O<sub>2</sub> treatment also caused a marked reduction in the expression of Bcl-2 while no significant change of Bax was observed. Treatment with taurine restored the reduced expression of Bcl-2 close to the control level without any obvious effect on Bax. Furthermore, taurine was also found to suppress up-regulation of GRP78, GADD153/CHOP and Bim induced by H<sub>2</sub>O<sub>2</sub>, suggesting that taurine may also exert a protective function against oxidative stress by reducing the ER stress.</p> <p>Conclusion</p> <p>In summary, taurine was shown to protect PC12 cells against oxidative stress induced by H<sub>2</sub>O<sub>2</sub>. ER stress was induced by oxidative stress and can be suppressed by taurine.</p> |
| spellingShingle | Pan Chunliu Giraldo Grace S Prentice Howard Wu Jang-Yen Taurine protection of PC12 cells against endoplasmic reticulum stress induced by oxidative stress Journal of Biomedical Science |
| title | Taurine protection of PC12 cells against endoplasmic reticulum stress induced by oxidative stress |
| title_full | Taurine protection of PC12 cells against endoplasmic reticulum stress induced by oxidative stress |
| title_fullStr | Taurine protection of PC12 cells against endoplasmic reticulum stress induced by oxidative stress |
| title_full_unstemmed | Taurine protection of PC12 cells against endoplasmic reticulum stress induced by oxidative stress |
| title_short | Taurine protection of PC12 cells against endoplasmic reticulum stress induced by oxidative stress |
| title_sort | taurine protection of pc12 cells against endoplasmic reticulum stress induced by oxidative stress |
| work_keys_str_mv | AT panchunliu taurineprotectionofpc12cellsagainstendoplasmicreticulumstressinducedbyoxidativestress AT giraldograces taurineprotectionofpc12cellsagainstendoplasmicreticulumstressinducedbyoxidativestress AT prenticehoward taurineprotectionofpc12cellsagainstendoplasmicreticulumstressinducedbyoxidativestress AT wujangyen taurineprotectionofpc12cellsagainstendoplasmicreticulumstressinducedbyoxidativestress |