Deeper below the surface—transcriptional changes in selected genes of Clostridium beijerinckii in response to butanol shock
Abstract The main bottleneck in the return of industrial butanol production from renewable feedstock through acetone–butanol–ethanol (ABE) fermentation by clostridia, such as Clostridium beijerinckii, is the low final butanol concentration. The problem is caused by the high toxicity of butanol to th...
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| Format: | Article |
| Language: | English |
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Wiley
2021-02-01
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| Series: | MicrobiologyOpen |
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| Online Access: | https://doi.org/10.1002/mbo3.1146 |
| _version_ | 1818740610038759424 |
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| author | Petra Patakova Jan Kolek Katerina Jureckova Barbora Branska Karel Sedlar Maryna Vasylkivska Ivo Provaznik |
| author_facet | Petra Patakova Jan Kolek Katerina Jureckova Barbora Branska Karel Sedlar Maryna Vasylkivska Ivo Provaznik |
| author_sort | Petra Patakova |
| collection | DOAJ |
| description | Abstract The main bottleneck in the return of industrial butanol production from renewable feedstock through acetone–butanol–ethanol (ABE) fermentation by clostridia, such as Clostridium beijerinckii, is the low final butanol concentration. The problem is caused by the high toxicity of butanol to the production cells, and therefore, understanding the mechanisms by which clostridia react to butanol shock is of key importance. Detailed analyses of transcriptome data that were obtained after butanol shock and their comparison with data from standard ABE fermentation have resulted in new findings, while confirmed expected population responses. Although butanol shock resulted in upregulation of heat shock protein genes, their regulation is different than was assumed based on standard ABE fermentation transcriptome data. While glucose uptake, glycolysis, and acidogenesis genes were downregulated after butanol shock, solventogenesis genes were upregulated. Cyclopropanation of fatty acids and formation of plasmalogens seem to be significant processes involved in cell membrane stabilization in the presence of butanol. Surprisingly, one of the three identified Agr quorum‐sensing system genes was upregulated. Upregulation of several putative butanol efflux pumps was described after butanol addition and a large putative polyketide gene cluster was found, the transcription of which seemed to depend on the concentration of butanol. |
| first_indexed | 2024-12-18T01:43:27Z |
| format | Article |
| id | doaj.art-de26b73d9cd948278a0f208766b5e506 |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-12-18T01:43:27Z |
| publishDate | 2021-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-de26b73d9cd948278a0f208766b5e5062022-12-21T21:25:15ZengWileyMicrobiologyOpen2045-88272021-02-01101n/an/a10.1002/mbo3.1146Deeper below the surface—transcriptional changes in selected genes of Clostridium beijerinckii in response to butanol shockPetra Patakova0Jan Kolek1Katerina Jureckova2Barbora Branska3Karel Sedlar4Maryna Vasylkivska5Ivo Provaznik6Department of Biotechnology University of Chemistry and Technology Prague Prague Czech RepublicDepartment of Biotechnology University of Chemistry and Technology Prague Prague Czech RepublicDepartment of Biomedical Engineering Brno University of Technology Brno Czech RepublicDepartment of Biotechnology University of Chemistry and Technology Prague Prague Czech RepublicDepartment of Biomedical Engineering Brno University of Technology Brno Czech RepublicDepartment of Biotechnology University of Chemistry and Technology Prague Prague Czech RepublicDepartment of Biomedical Engineering Brno University of Technology Brno Czech RepublicAbstract The main bottleneck in the return of industrial butanol production from renewable feedstock through acetone–butanol–ethanol (ABE) fermentation by clostridia, such as Clostridium beijerinckii, is the low final butanol concentration. The problem is caused by the high toxicity of butanol to the production cells, and therefore, understanding the mechanisms by which clostridia react to butanol shock is of key importance. Detailed analyses of transcriptome data that were obtained after butanol shock and their comparison with data from standard ABE fermentation have resulted in new findings, while confirmed expected population responses. Although butanol shock resulted in upregulation of heat shock protein genes, their regulation is different than was assumed based on standard ABE fermentation transcriptome data. While glucose uptake, glycolysis, and acidogenesis genes were downregulated after butanol shock, solventogenesis genes were upregulated. Cyclopropanation of fatty acids and formation of plasmalogens seem to be significant processes involved in cell membrane stabilization in the presence of butanol. Surprisingly, one of the three identified Agr quorum‐sensing system genes was upregulated. Upregulation of several putative butanol efflux pumps was described after butanol addition and a large putative polyketide gene cluster was found, the transcription of which seemed to depend on the concentration of butanol.https://doi.org/10.1002/mbo3.1146ABE fermentationbutanol shockClostridium beijerinckiitranscriptome analysis |
| spellingShingle | Petra Patakova Jan Kolek Katerina Jureckova Barbora Branska Karel Sedlar Maryna Vasylkivska Ivo Provaznik Deeper below the surface—transcriptional changes in selected genes of Clostridium beijerinckii in response to butanol shock MicrobiologyOpen ABE fermentation butanol shock Clostridium beijerinckii transcriptome analysis |
| title | Deeper below the surface—transcriptional changes in selected genes of Clostridium beijerinckii in response to butanol shock |
| title_full | Deeper below the surface—transcriptional changes in selected genes of Clostridium beijerinckii in response to butanol shock |
| title_fullStr | Deeper below the surface—transcriptional changes in selected genes of Clostridium beijerinckii in response to butanol shock |
| title_full_unstemmed | Deeper below the surface—transcriptional changes in selected genes of Clostridium beijerinckii in response to butanol shock |
| title_short | Deeper below the surface—transcriptional changes in selected genes of Clostridium beijerinckii in response to butanol shock |
| title_sort | deeper below the surface transcriptional changes in selected genes of clostridium beijerinckii in response to butanol shock |
| topic | ABE fermentation butanol shock Clostridium beijerinckii transcriptome analysis |
| url | https://doi.org/10.1002/mbo3.1146 |
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