Network pharmacology-based approach to investigate the molecular targets of essential oil obtained from lavender for treating breast cancer
Lavender essential oil (LEO) is known for its medicinal use in the development of pharmaceuticals. Further investigations were demonstrated that LEO has many biological properties including apoptosis. However, The anti-breast cancer activity and mechanism of LEO are still unclear. we aim to elucidat...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023089673 |
| _version_ | 1797429793037221888 |
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| author | Guzhalinuer Maitisha Junhao Zhou Yan Zhao Shuxia Han Youyun Zhao Ablikim Abliz Guangzhong Liu |
| author_facet | Guzhalinuer Maitisha Junhao Zhou Yan Zhao Shuxia Han Youyun Zhao Ablikim Abliz Guangzhong Liu |
| author_sort | Guzhalinuer Maitisha |
| collection | DOAJ |
| description | Lavender essential oil (LEO) is known for its medicinal use in the development of pharmaceuticals. Further investigations were demonstrated that LEO has many biological properties including apoptosis. However, The anti-breast cancer activity and mechanism of LEO are still unclear. we aim to elucidate the elusive anti-breast cancer activity and mechanism of LEO by unveiling the intricate molecular targets that it engages with, thereby priming it for effective therapeutic intervention against breast carcinoma. In this paper, we extracted LEO from lavender and analyzed it's chemical constituents by using hydro-distillation and gas chromatography-mass spectrometry (GS-MS/MS) method, respectively. The active components against breast cancer and it's molecular targets were selected and biological process, molecular function, cellular component and involving pathways were evaluated via network pharmacology approach. Cell viability, apoptosis and cell cycle assay were used to evaluate anti-breast cancer effect of LEO. Employing the western blotting method to validate target protein expression following LEO treatment in vitro. We found the 21 effective components and 213 drug-disease common targets of LEO. Amoung them, 7 active components and 19 targets were identified as potential therapeutic targets. Gene ontology results revealed that the drug-disease common targets of LEO were mainly distributed in membrane region, involved in peptide-tyrosine phosphorylation, and primarily associated with protein tyrosine kinase. We also found that drug-disease common targets might contribute to the regulation of PI3K-AKT signaling pathway by using KEGG pathway analysis. Besides, our study demonstrated reduced cell viability, induced apoptosis in MCF-7 and MDA-MB-231 treated with LEO while cell cycle arrest was not altered. The AKT1 expression down-regulated while PIK3CA expression was increased in both cell lines. Our findings indicate that LEO has the ability to induce apoptosis by modulating the expression of PI3K-AKT signaling pathway in these cell lines. |
| first_indexed | 2024-03-09T09:19:17Z |
| format | Article |
| id | doaj.art-de27ea7688f54a1a8044e7086002361f |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:19:17Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-de27ea7688f54a1a8044e7086002361f2023-12-02T07:04:08ZengElsevierHeliyon2405-84402023-11-01911e21759Network pharmacology-based approach to investigate the molecular targets of essential oil obtained from lavender for treating breast cancerGuzhalinuer Maitisha0Junhao Zhou1Yan Zhao2Shuxia Han3Youyun Zhao4Ablikim Abliz5Guangzhong Liu6Clinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Province Academy of Traditional Chinese Medicine, Wuhan 430061, ChinaSchool of Laboratory Medicine, Hubei University of Chinese Medicine, Wuhan 430065, ChinaClinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Province Academy of Traditional Chinese Medicine, Wuhan 430061, ChinaClinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Province Academy of Traditional Chinese Medicine, Wuhan 430061, ChinaClinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Province Academy of Traditional Chinese Medicine, Wuhan 430061, China; Corresponding author.Department of Gastrointestinal Surgery, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Province Academy of Traditional Chinese Medicine, Wuhan 430061, China; Corresponding author.Clinical Laboratory, Hubei Provincial Hospital of Traditional Chinese Medicine, Hubei Province Academy of Traditional Chinese Medicine, Wuhan 430061, China; Corresponding author.Lavender essential oil (LEO) is known for its medicinal use in the development of pharmaceuticals. Further investigations were demonstrated that LEO has many biological properties including apoptosis. However, The anti-breast cancer activity and mechanism of LEO are still unclear. we aim to elucidate the elusive anti-breast cancer activity and mechanism of LEO by unveiling the intricate molecular targets that it engages with, thereby priming it for effective therapeutic intervention against breast carcinoma. In this paper, we extracted LEO from lavender and analyzed it's chemical constituents by using hydro-distillation and gas chromatography-mass spectrometry (GS-MS/MS) method, respectively. The active components against breast cancer and it's molecular targets were selected and biological process, molecular function, cellular component and involving pathways were evaluated via network pharmacology approach. Cell viability, apoptosis and cell cycle assay were used to evaluate anti-breast cancer effect of LEO. Employing the western blotting method to validate target protein expression following LEO treatment in vitro. We found the 21 effective components and 213 drug-disease common targets of LEO. Amoung them, 7 active components and 19 targets were identified as potential therapeutic targets. Gene ontology results revealed that the drug-disease common targets of LEO were mainly distributed in membrane region, involved in peptide-tyrosine phosphorylation, and primarily associated with protein tyrosine kinase. We also found that drug-disease common targets might contribute to the regulation of PI3K-AKT signaling pathway by using KEGG pathway analysis. Besides, our study demonstrated reduced cell viability, induced apoptosis in MCF-7 and MDA-MB-231 treated with LEO while cell cycle arrest was not altered. The AKT1 expression down-regulated while PIK3CA expression was increased in both cell lines. Our findings indicate that LEO has the ability to induce apoptosis by modulating the expression of PI3K-AKT signaling pathway in these cell lines.http://www.sciencedirect.com/science/article/pii/S2405844023089673Lavender essential oilBreast cancerNetwork pharmacologyMolecular targetsPI3K-AKT signaling pathway |
| spellingShingle | Guzhalinuer Maitisha Junhao Zhou Yan Zhao Shuxia Han Youyun Zhao Ablikim Abliz Guangzhong Liu Network pharmacology-based approach to investigate the molecular targets of essential oil obtained from lavender for treating breast cancer Heliyon Lavender essential oil Breast cancer Network pharmacology Molecular targets PI3K-AKT signaling pathway |
| title | Network pharmacology-based approach to investigate the molecular targets of essential oil obtained from lavender for treating breast cancer |
| title_full | Network pharmacology-based approach to investigate the molecular targets of essential oil obtained from lavender for treating breast cancer |
| title_fullStr | Network pharmacology-based approach to investigate the molecular targets of essential oil obtained from lavender for treating breast cancer |
| title_full_unstemmed | Network pharmacology-based approach to investigate the molecular targets of essential oil obtained from lavender for treating breast cancer |
| title_short | Network pharmacology-based approach to investigate the molecular targets of essential oil obtained from lavender for treating breast cancer |
| title_sort | network pharmacology based approach to investigate the molecular targets of essential oil obtained from lavender for treating breast cancer |
| topic | Lavender essential oil Breast cancer Network pharmacology Molecular targets PI3K-AKT signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023089673 |
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