DJ-1 and SOD1 Act Independently in the Protection against Anoxia in <i>Drosophila melanogaster</i>
Redox homeostasis is a vital process the maintenance of which is assured by the presence of numerous antioxidant small molecules and enzymes and the alteration of which is involved in many pathologies, including several neurodegenerative disorders. Among the different enzymes involved in the antioxi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/8/1527 |
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| author | Federica De Lazzari Francesco Agostini Davide Doni Sandro Malacrida Mauro A. Zordan Paola Costantini Luigi Bubacco Federica Sandrelli Marco Bisaglia |
| author_facet | Federica De Lazzari Francesco Agostini Davide Doni Sandro Malacrida Mauro A. Zordan Paola Costantini Luigi Bubacco Federica Sandrelli Marco Bisaglia |
| author_sort | Federica De Lazzari |
| collection | DOAJ |
| description | Redox homeostasis is a vital process the maintenance of which is assured by the presence of numerous antioxidant small molecules and enzymes and the alteration of which is involved in many pathologies, including several neurodegenerative disorders. Among the different enzymes involved in the antioxidant response, SOD1 and DJ-1 have both been associated with the pathogenesis of amyotrophic lateral sclerosis and Parkinson’s disease, suggesting a possible interplay in their mechanism of action. Copper deficiency in the SOD1-active site has been proposed as a central determinant in SOD1-related neurodegeneration. SOD1 maturation mainly relies on the presence of the protein copper chaperone for SOD1 (CCS), but a CCS-independent alternative pathway also exists and functions under anaerobic conditions. To explore the possible involvement of DJ-1 in such a pathway in vivo, we exposed <i>Drosophila melanogaster</i> to anoxia and evaluated the effect of DJ-1 on fly survival and SOD1 levels, in the presence or absence of CCS. Loss of DJ-1 negatively affects the fly response to the anoxic treatment, but our data indicate that the protective activity of DJ-1 is independent of SOD1 in <i>Drosophila</i>, indicating that the two proteins may act in different pathways. |
| first_indexed | 2024-03-09T10:02:54Z |
| format | Article |
| id | doaj.art-de29a44c50724b02bb1509449a03ab1a |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T10:02:54Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-de29a44c50724b02bb1509449a03ab1a2023-12-01T23:20:08ZengMDPI AGAntioxidants2076-39212022-08-01118152710.3390/antiox11081527DJ-1 and SOD1 Act Independently in the Protection against Anoxia in <i>Drosophila melanogaster</i>Federica De Lazzari0Francesco Agostini1Davide Doni2Sandro Malacrida3Mauro A. Zordan4Paola Costantini5Luigi Bubacco6Federica Sandrelli7Marco Bisaglia8Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy Institute of Mountain Emergency Medicine, Eurac Research, 39100 Bolzano, ItalyDepartment of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy Department of Biology, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy Redox homeostasis is a vital process the maintenance of which is assured by the presence of numerous antioxidant small molecules and enzymes and the alteration of which is involved in many pathologies, including several neurodegenerative disorders. Among the different enzymes involved in the antioxidant response, SOD1 and DJ-1 have both been associated with the pathogenesis of amyotrophic lateral sclerosis and Parkinson’s disease, suggesting a possible interplay in their mechanism of action. Copper deficiency in the SOD1-active site has been proposed as a central determinant in SOD1-related neurodegeneration. SOD1 maturation mainly relies on the presence of the protein copper chaperone for SOD1 (CCS), but a CCS-independent alternative pathway also exists and functions under anaerobic conditions. To explore the possible involvement of DJ-1 in such a pathway in vivo, we exposed <i>Drosophila melanogaster</i> to anoxia and evaluated the effect of DJ-1 on fly survival and SOD1 levels, in the presence or absence of CCS. Loss of DJ-1 negatively affects the fly response to the anoxic treatment, but our data indicate that the protective activity of DJ-1 is independent of SOD1 in <i>Drosophila</i>, indicating that the two proteins may act in different pathways.https://www.mdpi.com/2076-3921/11/8/1527amyotrophic lateral sclerosisDJ-1<i>Drosophila melanogaster</i>Parkinson’s diseaseSOD1 |
| spellingShingle | Federica De Lazzari Francesco Agostini Davide Doni Sandro Malacrida Mauro A. Zordan Paola Costantini Luigi Bubacco Federica Sandrelli Marco Bisaglia DJ-1 and SOD1 Act Independently in the Protection against Anoxia in <i>Drosophila melanogaster</i> Antioxidants amyotrophic lateral sclerosis DJ-1 <i>Drosophila melanogaster</i> Parkinson’s disease SOD1 |
| title | DJ-1 and SOD1 Act Independently in the Protection against Anoxia in <i>Drosophila melanogaster</i> |
| title_full | DJ-1 and SOD1 Act Independently in the Protection against Anoxia in <i>Drosophila melanogaster</i> |
| title_fullStr | DJ-1 and SOD1 Act Independently in the Protection against Anoxia in <i>Drosophila melanogaster</i> |
| title_full_unstemmed | DJ-1 and SOD1 Act Independently in the Protection against Anoxia in <i>Drosophila melanogaster</i> |
| title_short | DJ-1 and SOD1 Act Independently in the Protection against Anoxia in <i>Drosophila melanogaster</i> |
| title_sort | dj 1 and sod1 act independently in the protection against anoxia in i drosophila melanogaster i |
| topic | amyotrophic lateral sclerosis DJ-1 <i>Drosophila melanogaster</i> Parkinson’s disease SOD1 |
| url | https://www.mdpi.com/2076-3921/11/8/1527 |
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