Characterization of Field-Evolved Resistance to Afidopyropen, a Novel Insecticidal Toxin Developed from Microbial Secondary Metabolites, in <i>Bemisia tabaci</i>
Afidopyropen, a newly identified chemical, is a derivative of pyripyropene A, which is produced by the filamentous fungus <i>Penicillium coprobium</i>. It is a promising novel pesticide applied against whiteflies in agriculture. In this study, the reversion and selection, cross-resistanc...
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MDPI AG
2022-07-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/7/453 |
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| author | Ran Wang Qinghe Zhang Xuan Zhou Mi Zhang Qingyi Yang Qi Su Chen Luo |
| author_facet | Ran Wang Qinghe Zhang Xuan Zhou Mi Zhang Qingyi Yang Qi Su Chen Luo |
| author_sort | Ran Wang |
| collection | DOAJ |
| description | Afidopyropen, a newly identified chemical, is a derivative of pyripyropene A, which is produced by the filamentous fungus <i>Penicillium coprobium</i>. It is a promising novel pesticide applied against whiteflies in agriculture. In this study, the reversion and selection, cross-resistance patterns, synergistic effects, and fitness costs of afidopyropen resistance were studied in a field-developed resistant population of <i>B. tabaci</i>. Compared to a reference MED-S strain, the field-developed resistant Haidian (HD) population showed 36.5-fold resistance to afidopyropen. Significant reversion of resistance to afidopyropen was found in the HD population when it was kept with no selective pressure of the insecticide. The HD-Afi strain, developed from the HD population with afidopyropen pressure, developed 104.3-fold resistance to afidopyropen and significant cross-resistance to sulfoxaflor. Piperonyl butoxide (PBO) largely inhibited afidopyropen resistance in the HD-Afi strain, which indicates that P450 monooxygenase could be involved in the resistance. Significant fitness costs associated with afidopyropen resistance were observed in HD-Afi. This study indicates that a rotation of afidopyropen with other chemical control agents could be useful for impeding afidopyropen resistance in <i>B. tabaci</i>. In addition, we expanded upon the understanding of resistance to afidopyropen, offering evidence suggesting the importance of devising better strategies for the management of whiteflies. |
| first_indexed | 2024-03-09T12:55:22Z |
| format | Article |
| id | doaj.art-de2b755945414e848ae6b421dcf82157 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T12:55:22Z |
| publishDate | 2022-07-01 |
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| record_format | Article |
| series | Toxins |
| spelling | doaj.art-de2b755945414e848ae6b421dcf821572023-11-30T22:01:19ZengMDPI AGToxins2072-66512022-07-0114745310.3390/toxins14070453Characterization of Field-Evolved Resistance to Afidopyropen, a Novel Insecticidal Toxin Developed from Microbial Secondary Metabolites, in <i>Bemisia tabaci</i>Ran Wang0Qinghe Zhang1Xuan Zhou2Mi Zhang3Qingyi Yang4Qi Su5Chen Luo6Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaHubei Engineering Technology Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou 434025, ChinaHubei Engineering Technology Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou 434025, ChinaCollege of Agriculture and Forestry Technology, Hebei North University, Zhangjiakou 075000, ChinaCollege of Agriculture and Forestry Technology, Hebei North University, Zhangjiakou 075000, ChinaHubei Engineering Technology Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou 434025, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaAfidopyropen, a newly identified chemical, is a derivative of pyripyropene A, which is produced by the filamentous fungus <i>Penicillium coprobium</i>. It is a promising novel pesticide applied against whiteflies in agriculture. In this study, the reversion and selection, cross-resistance patterns, synergistic effects, and fitness costs of afidopyropen resistance were studied in a field-developed resistant population of <i>B. tabaci</i>. Compared to a reference MED-S strain, the field-developed resistant Haidian (HD) population showed 36.5-fold resistance to afidopyropen. Significant reversion of resistance to afidopyropen was found in the HD population when it was kept with no selective pressure of the insecticide. The HD-Afi strain, developed from the HD population with afidopyropen pressure, developed 104.3-fold resistance to afidopyropen and significant cross-resistance to sulfoxaflor. Piperonyl butoxide (PBO) largely inhibited afidopyropen resistance in the HD-Afi strain, which indicates that P450 monooxygenase could be involved in the resistance. Significant fitness costs associated with afidopyropen resistance were observed in HD-Afi. This study indicates that a rotation of afidopyropen with other chemical control agents could be useful for impeding afidopyropen resistance in <i>B. tabaci</i>. In addition, we expanded upon the understanding of resistance to afidopyropen, offering evidence suggesting the importance of devising better strategies for the management of whiteflies.https://www.mdpi.com/2072-6651/14/7/453<i>Bemisia tabaci</i>afidopyropeninsecticidal toxincross-resistancesynergismfitness costs |
| spellingShingle | Ran Wang Qinghe Zhang Xuan Zhou Mi Zhang Qingyi Yang Qi Su Chen Luo Characterization of Field-Evolved Resistance to Afidopyropen, a Novel Insecticidal Toxin Developed from Microbial Secondary Metabolites, in <i>Bemisia tabaci</i> Toxins <i>Bemisia tabaci</i> afidopyropen insecticidal toxin cross-resistance synergism fitness costs |
| title | Characterization of Field-Evolved Resistance to Afidopyropen, a Novel Insecticidal Toxin Developed from Microbial Secondary Metabolites, in <i>Bemisia tabaci</i> |
| title_full | Characterization of Field-Evolved Resistance to Afidopyropen, a Novel Insecticidal Toxin Developed from Microbial Secondary Metabolites, in <i>Bemisia tabaci</i> |
| title_fullStr | Characterization of Field-Evolved Resistance to Afidopyropen, a Novel Insecticidal Toxin Developed from Microbial Secondary Metabolites, in <i>Bemisia tabaci</i> |
| title_full_unstemmed | Characterization of Field-Evolved Resistance to Afidopyropen, a Novel Insecticidal Toxin Developed from Microbial Secondary Metabolites, in <i>Bemisia tabaci</i> |
| title_short | Characterization of Field-Evolved Resistance to Afidopyropen, a Novel Insecticidal Toxin Developed from Microbial Secondary Metabolites, in <i>Bemisia tabaci</i> |
| title_sort | characterization of field evolved resistance to afidopyropen a novel insecticidal toxin developed from microbial secondary metabolites in i bemisia tabaci i |
| topic | <i>Bemisia tabaci</i> afidopyropen insecticidal toxin cross-resistance synergism fitness costs |
| url | https://www.mdpi.com/2072-6651/14/7/453 |
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