Myography of isolated blood vessels: Considerations for experimental design and combination with supplementary techniques
The study of the mechanisms of regulation of vascular tone is an urgent task of modern science, since diseases of the cardiovascular system remain the main cause of reduction in the quality of life and mortality of the population. Myography (isometric and isobaric) of isolated blood vessels is one o...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Physiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2023.1176748/full |
| _version_ | 1797826177656684544 |
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| author | Rudolf Schubert Dina Gaynullina Anastasia Shvetsova Olga S. Tarasova Olga S. Tarasova |
| author_facet | Rudolf Schubert Dina Gaynullina Anastasia Shvetsova Olga S. Tarasova Olga S. Tarasova |
| author_sort | Rudolf Schubert |
| collection | DOAJ |
| description | The study of the mechanisms of regulation of vascular tone is an urgent task of modern science, since diseases of the cardiovascular system remain the main cause of reduction in the quality of life and mortality of the population. Myography (isometric and isobaric) of isolated blood vessels is one of the most physiologically relevant approaches to study the function of cells in the vessel wall. On the one hand, cell-cell interactions as well as mechanical stretch of the vessel wall remain preserved in myography studies, in contrast to studies on isolated cells, e.g., cell culture. On the other hand, in vitro studies in isolated vessels allow control of numerous parameters that are difficult to control in vivo. The aim of this review was to 1) discuss the specifics of experimental design and interpretation of data obtained by myography and 2) highlight the importance of the combined use of myography with various complementary techniques necessary for a deep understanding of vascular physiology. |
| first_indexed | 2024-03-13T11:04:53Z |
| format | Article |
| id | doaj.art-de2ca02f279847efaa54f1bf308fc7e2 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-03-13T11:04:53Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-de2ca02f279847efaa54f1bf308fc7e22023-05-16T11:42:58ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2023-04-011410.3389/fphys.2023.11767481176748Myography of isolated blood vessels: Considerations for experimental design and combination with supplementary techniquesRudolf Schubert0Dina Gaynullina1Anastasia Shvetsova2Olga S. Tarasova3Olga S. Tarasova4Physiology, Institute of Theoretical Medicine, Faculty of Medicine, University of Augsburg, Augsburg, GermanyFaculty of Biology, M.V. Lomonosov Moscow State University, Moscow, RussiaFaculty of Biology, M.V. Lomonosov Moscow State University, Moscow, RussiaFaculty of Biology, M.V. Lomonosov Moscow State University, Moscow, RussiaState Research Center of the Russian Federation, Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, RussiaThe study of the mechanisms of regulation of vascular tone is an urgent task of modern science, since diseases of the cardiovascular system remain the main cause of reduction in the quality of life and mortality of the population. Myography (isometric and isobaric) of isolated blood vessels is one of the most physiologically relevant approaches to study the function of cells in the vessel wall. On the one hand, cell-cell interactions as well as mechanical stretch of the vessel wall remain preserved in myography studies, in contrast to studies on isolated cells, e.g., cell culture. On the other hand, in vitro studies in isolated vessels allow control of numerous parameters that are difficult to control in vivo. The aim of this review was to 1) discuss the specifics of experimental design and interpretation of data obtained by myography and 2) highlight the importance of the combined use of myography with various complementary techniques necessary for a deep understanding of vascular physiology.https://www.frontiersin.org/articles/10.3389/fphys.2023.1176748/fullmyographyarterysmooth muscleendotheliummembrane potentialintracellular calcium |
| spellingShingle | Rudolf Schubert Dina Gaynullina Anastasia Shvetsova Olga S. Tarasova Olga S. Tarasova Myography of isolated blood vessels: Considerations for experimental design and combination with supplementary techniques Frontiers in Physiology myography artery smooth muscle endothelium membrane potential intracellular calcium |
| title | Myography of isolated blood vessels: Considerations for experimental design and combination with supplementary techniques |
| title_full | Myography of isolated blood vessels: Considerations for experimental design and combination with supplementary techniques |
| title_fullStr | Myography of isolated blood vessels: Considerations for experimental design and combination with supplementary techniques |
| title_full_unstemmed | Myography of isolated blood vessels: Considerations for experimental design and combination with supplementary techniques |
| title_short | Myography of isolated blood vessels: Considerations for experimental design and combination with supplementary techniques |
| title_sort | myography of isolated blood vessels considerations for experimental design and combination with supplementary techniques |
| topic | myography artery smooth muscle endothelium membrane potential intracellular calcium |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2023.1176748/full |
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