Experimental observation of the effect of immunotherapy on CD4+ T cells and Th1/Th2 cytokines in mice with allergic rhinitis
Abstract The present study aims to investigate the effect of immunotherapy in a mouse model of allergic rhinitis (AR) and to explore the possible molecular mechanisms of action. An animal model of AR was established by sensitization and challenge of BALB/c mice with house dust mite (HDM) extract. Th...
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Nature Portfolio
2023-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-32507-6 |
| _version_ | 1827939938111324160 |
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| author | Yu Zhu Juan Yu XinHua Zhu JiaSheng Yuan MeiNa Dai YouWei Bao YinLi Jiang |
| author_facet | Yu Zhu Juan Yu XinHua Zhu JiaSheng Yuan MeiNa Dai YouWei Bao YinLi Jiang |
| author_sort | Yu Zhu |
| collection | DOAJ |
| description | Abstract The present study aims to investigate the effect of immunotherapy in a mouse model of allergic rhinitis (AR) and to explore the possible molecular mechanisms of action. An animal model of AR was established by sensitization and challenge of BALB/c mice with house dust mite (HDM) extract. The mice were injected subcutaneously with HDM for immunotherapy. AR nasal symptoms were evaluated according to the frequencies of nose rubbing and sneezing and the degree of rhinorrhea. The nasal mucosa and lung tissue architecture and inflammatory status by histological analysis; the infiltration of eosinophils in nasal lavage fluid (NALF) of mice was observed by Diff-Quik stain; ELISA-based quantification of serum HDM-specific IgE and TH1/TH2 cytokine concentration; and flow cytometry detected the number of serum CD4+/CD8+ cells to evaluate the mechanism of immunotherapy. It was found that after immunotherapy, the AR symptom score was reduced, the number of eosinophils in NALF was reduced, and the infiltration of inflammatory cells and tissue damage in the nasal mucosa and lung tissue were alleviated. Immunotherapy can increase the number of CD4+ T cells in the peripheral blood, increase the ratio of CD4+/CD8+ cells, increase the expression of Th1 cytokines such as IL-2 and IFN-γ, reduce the expression of Th2 cytokines such as IL-4 and IL-5. The results showed that repeated intraperitoneal injection of crude extract of HDM for sensitization, followed by nasal drops can effectively construct a mouse model of AR, and subcutaneous injection of immunotherapy in mice can reduce allergic inflammation in model mice and improve the inflammatory infiltration of the nasal cavity in allergic rhinitis. Immunotherapy can reduce the expression of inflammatory factors in AR, improve Th1/Th2 balance, and may play a role in the treatment of AR by improving the function of immune cells. |
| first_indexed | 2024-03-13T09:02:07Z |
| format | Article |
| id | doaj.art-de2dbad252c54acca0eb49a5fd244af7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T09:02:07Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-de2dbad252c54acca0eb49a5fd244af72023-05-28T11:16:13ZengNature PortfolioScientific Reports2045-23222023-03-011311910.1038/s41598-023-32507-6Experimental observation of the effect of immunotherapy on CD4+ T cells and Th1/Th2 cytokines in mice with allergic rhinitisYu Zhu0Juan Yu1XinHua Zhu2JiaSheng Yuan3MeiNa Dai4YouWei Bao5YinLi Jiang6Department of Otolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Nanchang UniversityDepartment of Otolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Nanchang UniversityDepartment of Otolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Nanchang UniversityDepartment of Otolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Nanchang UniversityDepartment of Otolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Nanchang UniversityDepartment of Otolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Nanchang UniversityDepartment of Otolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Nanchang UniversityAbstract The present study aims to investigate the effect of immunotherapy in a mouse model of allergic rhinitis (AR) and to explore the possible molecular mechanisms of action. An animal model of AR was established by sensitization and challenge of BALB/c mice with house dust mite (HDM) extract. The mice were injected subcutaneously with HDM for immunotherapy. AR nasal symptoms were evaluated according to the frequencies of nose rubbing and sneezing and the degree of rhinorrhea. The nasal mucosa and lung tissue architecture and inflammatory status by histological analysis; the infiltration of eosinophils in nasal lavage fluid (NALF) of mice was observed by Diff-Quik stain; ELISA-based quantification of serum HDM-specific IgE and TH1/TH2 cytokine concentration; and flow cytometry detected the number of serum CD4+/CD8+ cells to evaluate the mechanism of immunotherapy. It was found that after immunotherapy, the AR symptom score was reduced, the number of eosinophils in NALF was reduced, and the infiltration of inflammatory cells and tissue damage in the nasal mucosa and lung tissue were alleviated. Immunotherapy can increase the number of CD4+ T cells in the peripheral blood, increase the ratio of CD4+/CD8+ cells, increase the expression of Th1 cytokines such as IL-2 and IFN-γ, reduce the expression of Th2 cytokines such as IL-4 and IL-5. The results showed that repeated intraperitoneal injection of crude extract of HDM for sensitization, followed by nasal drops can effectively construct a mouse model of AR, and subcutaneous injection of immunotherapy in mice can reduce allergic inflammation in model mice and improve the inflammatory infiltration of the nasal cavity in allergic rhinitis. Immunotherapy can reduce the expression of inflammatory factors in AR, improve Th1/Th2 balance, and may play a role in the treatment of AR by improving the function of immune cells.https://doi.org/10.1038/s41598-023-32507-6 |
| spellingShingle | Yu Zhu Juan Yu XinHua Zhu JiaSheng Yuan MeiNa Dai YouWei Bao YinLi Jiang Experimental observation of the effect of immunotherapy on CD4+ T cells and Th1/Th2 cytokines in mice with allergic rhinitis Scientific Reports |
| title | Experimental observation of the effect of immunotherapy on CD4+ T cells and Th1/Th2 cytokines in mice with allergic rhinitis |
| title_full | Experimental observation of the effect of immunotherapy on CD4+ T cells and Th1/Th2 cytokines in mice with allergic rhinitis |
| title_fullStr | Experimental observation of the effect of immunotherapy on CD4+ T cells and Th1/Th2 cytokines in mice with allergic rhinitis |
| title_full_unstemmed | Experimental observation of the effect of immunotherapy on CD4+ T cells and Th1/Th2 cytokines in mice with allergic rhinitis |
| title_short | Experimental observation of the effect of immunotherapy on CD4+ T cells and Th1/Th2 cytokines in mice with allergic rhinitis |
| title_sort | experimental observation of the effect of immunotherapy on cd4 t cells and th1 th2 cytokines in mice with allergic rhinitis |
| url | https://doi.org/10.1038/s41598-023-32507-6 |
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