Protective effect of royal jelly in 2,4,6 trinitrobenzene sulfonic acid-induced colitis in rats
Objective(s):In the present study, we evaluated immunological and immunomodulatory properties of royal jelly (RJ) in 2,4,6 trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats. Materials and Methods: Eighteen adult female Wistar albino rats were divided into three groups of six animals each:...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2015-04-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | http://ijbms.mums.ac.ir/pdf_4286_bda319ebeacacd60c57dac499c123a58.html |
| _version_ | 1828814525438099456 |
|---|---|
| author | Turan Karaca Yesim Hulya Uz Selim Demirtas Ihsan Karaboga Guray Can |
| author_facet | Turan Karaca Yesim Hulya Uz Selim Demirtas Ihsan Karaboga Guray Can |
| author_sort | Turan Karaca |
| collection | DOAJ |
| description | Objective(s):In the present study, we evaluated immunological and immunomodulatory properties of royal jelly (RJ) in 2,4,6 trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats.
Materials and Methods: Eighteen adult female Wistar albino rats were divided into three groups of six animals each: a control group that received only saline solution, a TNBS-induced colitis group, and a TNBS-colitis+RJ group that received 250 mg/kg/day of RJ for seven days before the induction of colitis, following by the same treatment for an additional seven days. At the end of the experiment, cardiac blood and colon samples were obtained under deep anaesthesia from the animals in all groups. Serum interleukin-1β (IL-1β), tumour necrosis factor-alpha (TNF-α) and IL-10 levels were analyzed with an enzyme-linked immunosorbent assay (ELISA). Five-micrometre-thick sections were stained with haematoxylin-eosin (H&E) for microscopic evaluations. For immunohistochemical evaluations, the paraffin sections were stained with anti-CD3 (cluster of differentiation), anti-CD5, anti-CD8 and anti-CD45.
Results: The results showed that the oral RJ treatment inhibited proinflammatory cytokines, IL-1β and TNF-α secretion, while increasing anti-inflammatory cytokine IL-10 production in the TNBS-induced colitis+RJ group compared with the colitis group not treated with RJ. The colitis was not as severe in the colitis+RJ group, with ulcerative damage, weight loss and inflammatory scores suggesting that impaired CD3-, CD5-, CD8- and CD45-positive T cell immune responses likely mediated the anti-inflammatory effect.
Conclusion: The antioxidant and anti-inflammatory properties of RJ protected colon mucosa against TNBS-induced colitis in rats orally treated with RJ. |
| first_indexed | 2024-12-12T10:24:38Z |
| format | Article |
| id | doaj.art-de2de4af18a04b66bb78ebce3a56e71a |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-12T10:24:38Z |
| publishDate | 2015-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-de2de4af18a04b66bb78ebce3a56e71a2022-12-22T00:27:28ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742015-04-011843703794286Protective effect of royal jelly in 2,4,6 trinitrobenzene sulfonic acid-induced colitis in ratsTuran Karaca0Yesim Hulya Uz1Selim Demirtas2Ihsan Karaboga3Guray Can4University of Trakya, Faculty of Medicine, Department of Histology- Embryology, Balkan Campus, 22030, Edirne, TurkeyUniversity of Trakya, Faculty of Medicine, Department of Histology- Embryology, Balkan Campus, 22030, Edirne, TurkeyUniversity of Trakya, Faculty of Medicine, Department of Histology- Embryology, Balkan Campus, 22030, Edirne, TurkeyUniversity of Trakya, Faculty of Medicine, Department of Histology- Embryology, Balkan Campus, 22030, Edirne, TurkeyUniversity of Trakya, Faculty of Medicine, Department of Gastroenterolgy, Balkan Campus, 22030, Edirne, TurkeyObjective(s):In the present study, we evaluated immunological and immunomodulatory properties of royal jelly (RJ) in 2,4,6 trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats. Materials and Methods: Eighteen adult female Wistar albino rats were divided into three groups of six animals each: a control group that received only saline solution, a TNBS-induced colitis group, and a TNBS-colitis+RJ group that received 250 mg/kg/day of RJ for seven days before the induction of colitis, following by the same treatment for an additional seven days. At the end of the experiment, cardiac blood and colon samples were obtained under deep anaesthesia from the animals in all groups. Serum interleukin-1β (IL-1β), tumour necrosis factor-alpha (TNF-α) and IL-10 levels were analyzed with an enzyme-linked immunosorbent assay (ELISA). Five-micrometre-thick sections were stained with haematoxylin-eosin (H&E) for microscopic evaluations. For immunohistochemical evaluations, the paraffin sections were stained with anti-CD3 (cluster of differentiation), anti-CD5, anti-CD8 and anti-CD45. Results: The results showed that the oral RJ treatment inhibited proinflammatory cytokines, IL-1β and TNF-α secretion, while increasing anti-inflammatory cytokine IL-10 production in the TNBS-induced colitis+RJ group compared with the colitis group not treated with RJ. The colitis was not as severe in the colitis+RJ group, with ulcerative damage, weight loss and inflammatory scores suggesting that impaired CD3-, CD5-, CD8- and CD45-positive T cell immune responses likely mediated the anti-inflammatory effect. Conclusion: The antioxidant and anti-inflammatory properties of RJ protected colon mucosa against TNBS-induced colitis in rats orally treated with RJ.http://ijbms.mums.ac.ir/pdf_4286_bda319ebeacacd60c57dac499c123a58.htmlColitisratsRoyal jellyTNBS |
| spellingShingle | Turan Karaca Yesim Hulya Uz Selim Demirtas Ihsan Karaboga Guray Can Protective effect of royal jelly in 2,4,6 trinitrobenzene sulfonic acid-induced colitis in rats Iranian Journal of Basic Medical Sciences Colitis rats Royal jelly TNBS |
| title | Protective effect of royal jelly in 2,4,6 trinitrobenzene sulfonic acid-induced colitis in rats |
| title_full | Protective effect of royal jelly in 2,4,6 trinitrobenzene sulfonic acid-induced colitis in rats |
| title_fullStr | Protective effect of royal jelly in 2,4,6 trinitrobenzene sulfonic acid-induced colitis in rats |
| title_full_unstemmed | Protective effect of royal jelly in 2,4,6 trinitrobenzene sulfonic acid-induced colitis in rats |
| title_short | Protective effect of royal jelly in 2,4,6 trinitrobenzene sulfonic acid-induced colitis in rats |
| title_sort | protective effect of royal jelly in 2 4 6 trinitrobenzene sulfonic acid induced colitis in rats |
| topic | Colitis rats Royal jelly TNBS |
| url | http://ijbms.mums.ac.ir/pdf_4286_bda319ebeacacd60c57dac499c123a58.html |
| work_keys_str_mv | AT turankaraca protectiveeffectofroyaljellyin246trinitrobenzenesulfonicacidinducedcolitisinrats AT yesimhulyauz protectiveeffectofroyaljellyin246trinitrobenzenesulfonicacidinducedcolitisinrats AT selimdemirtas protectiveeffectofroyaljellyin246trinitrobenzenesulfonicacidinducedcolitisinrats AT ihsankaraboga protectiveeffectofroyaljellyin246trinitrobenzenesulfonicacidinducedcolitisinrats AT guraycan protectiveeffectofroyaljellyin246trinitrobenzenesulfonicacidinducedcolitisinrats |