Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production
Excessive nitric oxide (NO) production by macrophages has been involved in inflammatory diseases. Seven polyphenols (1–7) were isolated from Broussonetia kazinoki (B. kazinoki) and investigated as potential inhibitors of NO overproduction in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Among...
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MDPI AG
2018-03-01
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author | Da Yeon Lee Hwa Jin Lee Jae-Ha Ryu |
author_facet | Da Yeon Lee Hwa Jin Lee Jae-Ha Ryu |
author_sort | Da Yeon Lee |
collection | DOAJ |
description | Excessive nitric oxide (NO) production by macrophages has been involved in inflammatory diseases. Seven polyphenols (1–7) were isolated from Broussonetia kazinoki (B. kazinoki) and investigated as potential inhibitors of NO overproduction in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Among them, four prenylated polyphenols (2–4 and 6) with a catechol moiety efficiently suppressed the LPS-induced high level of NO with IC50 values of less than 6 µM. The compounds 2–4 and 6 also attenuated protein and mRNA levels of inducible nitric oxide synthase (iNOS). Moreover, they suppressed the nuclear factor κB (NF-κB) activity by inhibiting the degradation of inhibitory-κB-α (I-κB-α) and the translocation of NF-κB into the nucleus in LPS-activated macrophages. Taken together, these findings suggest that polyphenols from B. kazinoki might be beneficial for treatment of inflammatory diseases. |
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issn | 1420-3049 |
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spelling | doaj.art-de2f126e14ca4b45a32a28c6c2748a742022-12-22T03:46:18ZengMDPI AGMolecules1420-30492018-03-0123363910.3390/molecules23030639molecules23030639Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide ProductionDa Yeon Lee0Hwa Jin Lee1Jae-Ha Ryu2College of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women’s University, Seoul 04310, KoreaSchool of Industrial Bio-Pharmaceutical Science, Semyung University, Jecheon, Chungbuk 27136, KoreaCollege of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women’s University, Seoul 04310, KoreaExcessive nitric oxide (NO) production by macrophages has been involved in inflammatory diseases. Seven polyphenols (1–7) were isolated from Broussonetia kazinoki (B. kazinoki) and investigated as potential inhibitors of NO overproduction in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Among them, four prenylated polyphenols (2–4 and 6) with a catechol moiety efficiently suppressed the LPS-induced high level of NO with IC50 values of less than 6 µM. The compounds 2–4 and 6 also attenuated protein and mRNA levels of inducible nitric oxide synthase (iNOS). Moreover, they suppressed the nuclear factor κB (NF-κB) activity by inhibiting the degradation of inhibitory-κB-α (I-κB-α) and the translocation of NF-κB into the nucleus in LPS-activated macrophages. Taken together, these findings suggest that polyphenols from B. kazinoki might be beneficial for treatment of inflammatory diseases.http://www.mdpi.com/1420-3049/23/3/639Broussonetia kazinokiprenylated polyphenolsnitric oxideinducible nitric oxide synthasenuclear factor κB |
spellingShingle | Da Yeon Lee Hwa Jin Lee Jae-Ha Ryu Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production Molecules Broussonetia kazinoki prenylated polyphenols nitric oxide inducible nitric oxide synthase nuclear factor κB |
title | Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production |
title_full | Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production |
title_fullStr | Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production |
title_full_unstemmed | Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production |
title_short | Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production |
title_sort | prenylated polyphenols from broussonetia kazinoki as inhibitors of nitric oxide production |
topic | Broussonetia kazinoki prenylated polyphenols nitric oxide inducible nitric oxide synthase nuclear factor κB |
url | http://www.mdpi.com/1420-3049/23/3/639 |
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