Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production

Excessive nitric oxide (NO) production by macrophages has been involved in inflammatory diseases. Seven polyphenols (1–7) were isolated from Broussonetia kazinoki (B. kazinoki) and investigated as potential inhibitors of NO overproduction in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Among...

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Main Authors: Da Yeon Lee, Hwa Jin Lee, Jae-Ha Ryu
Format: Article
Language:English
Published: MDPI AG 2018-03-01
Series:Molecules
Subjects:
Online Access:http://www.mdpi.com/1420-3049/23/3/639
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author Da Yeon Lee
Hwa Jin Lee
Jae-Ha Ryu
author_facet Da Yeon Lee
Hwa Jin Lee
Jae-Ha Ryu
author_sort Da Yeon Lee
collection DOAJ
description Excessive nitric oxide (NO) production by macrophages has been involved in inflammatory diseases. Seven polyphenols (1–7) were isolated from Broussonetia kazinoki (B. kazinoki) and investigated as potential inhibitors of NO overproduction in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Among them, four prenylated polyphenols (2–4 and 6) with a catechol moiety efficiently suppressed the LPS-induced high level of NO with IC50 values of less than 6 µM. The compounds 2–4 and 6 also attenuated protein and mRNA levels of inducible nitric oxide synthase (iNOS). Moreover, they suppressed the nuclear factor κB (NF-κB) activity by inhibiting the degradation of inhibitory-κB-α (I-κB-α) and the translocation of NF-κB into the nucleus in LPS-activated macrophages. Taken together, these findings suggest that polyphenols from B. kazinoki might be beneficial for treatment of inflammatory diseases.
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spelling doaj.art-de2f126e14ca4b45a32a28c6c2748a742022-12-22T03:46:18ZengMDPI AGMolecules1420-30492018-03-0123363910.3390/molecules23030639molecules23030639Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide ProductionDa Yeon Lee0Hwa Jin Lee1Jae-Ha Ryu2College of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women’s University, Seoul 04310, KoreaSchool of Industrial Bio-Pharmaceutical Science, Semyung University, Jecheon, Chungbuk 27136, KoreaCollege of Pharmacy and Research Center for Cell Fate Control, Sookmyung Women’s University, Seoul 04310, KoreaExcessive nitric oxide (NO) production by macrophages has been involved in inflammatory diseases. Seven polyphenols (1–7) were isolated from Broussonetia kazinoki (B. kazinoki) and investigated as potential inhibitors of NO overproduction in lipopolysaccharide (LPS)-activated RAW 264.7 cells. Among them, four prenylated polyphenols (2–4 and 6) with a catechol moiety efficiently suppressed the LPS-induced high level of NO with IC50 values of less than 6 µM. The compounds 2–4 and 6 also attenuated protein and mRNA levels of inducible nitric oxide synthase (iNOS). Moreover, they suppressed the nuclear factor κB (NF-κB) activity by inhibiting the degradation of inhibitory-κB-α (I-κB-α) and the translocation of NF-κB into the nucleus in LPS-activated macrophages. Taken together, these findings suggest that polyphenols from B. kazinoki might be beneficial for treatment of inflammatory diseases.http://www.mdpi.com/1420-3049/23/3/639Broussonetia kazinokiprenylated polyphenolsnitric oxideinducible nitric oxide synthasenuclear factor κB
spellingShingle Da Yeon Lee
Hwa Jin Lee
Jae-Ha Ryu
Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production
Molecules
Broussonetia kazinoki
prenylated polyphenols
nitric oxide
inducible nitric oxide synthase
nuclear factor κB
title Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production
title_full Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production
title_fullStr Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production
title_full_unstemmed Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production
title_short Prenylated Polyphenols from Broussonetia kazinoki as Inhibitors of Nitric Oxide Production
title_sort prenylated polyphenols from broussonetia kazinoki as inhibitors of nitric oxide production
topic Broussonetia kazinoki
prenylated polyphenols
nitric oxide
inducible nitric oxide synthase
nuclear factor κB
url http://www.mdpi.com/1420-3049/23/3/639
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AT jaeharyu prenylatedpolyphenolsfrombroussonetiakazinokiasinhibitorsofnitricoxideproduction